Microbiota of a Full-scale UASB Reactor Treating Brewery Wastewater Using Illumina MiSeq Sequencing

Abstract

Background:The efficiency of biological wastewater treatment plant is determined by bacterial metabolism. There are data on the effect of operational parameters on microbial consortia present in laboratory scale reactor. However, knowledge on the full-scale reactor is still limited at present, hence the need to define the relations between the microbial structure and the performance of full-scale reactor. Objective:In this study, the microbial community structure in a full-scale UASB reactor treating brewery wastewater was assessed using metagenomics Next-Generation Sequencing technique. Method:Granular sludge samples were collected from the UASB reactor treating brewery wastewater and extracted genomic DNA was amplified using barcoded bacterial primer sets targeting V3-V4 region of the 16S rRNA genes on sequencing Illumina MiSeq platform. Results:The taxonomic analysis revealed the abundance of bacteria (~95%) with considerable Archaea community (~2%) in the granular sludge. After trimming, 18 bacterial phyla, 29 orders, 36 families and 44 genera were recovered from the 48,488 sequences reads of the 16S rRNA genes analysed, where the most abundant community belongs to Firmicutes, Bacteroidetes, Synergistetes and Proteobacteria phyla. Conclusion:For a sustainable bioenergy generation, understanding the mechanisms of anaerobic system in relation to microbial community is an important factor to increase the production of biogas production during wastewater treatment. To the best of our knowledge, this report is one of the studies that explored and described bacterial diversity and community structure of a full-scale UASB reactor treating brewery wastewater using highthroughput sequencing. This study provides insight into the dominant microbial community and their phylogenetic diversity in biogas producing reactor.

Characterization of the Spatial Variation of Microbial Communities in a Decentralized Subtropical Wastewater Treatment Plant Using Passive Methods

“…The presence of Betaproteobacteria class members, such as Desulfobacteraceae and Desulfobulbacteraceae, was observed in all three units, with similar abundance (Figures 7a and 8a). Members of the phylum Proteobacteria, such as Betaproteobacteria and Deltaproteobacteria, have been reported to participate in the sulfur cycle in CW [22] and anaerobic digestion processes [68,89]. In addition, Meyer et al [68] found that the presence of sulfur, potassium, manganese, total nitrogen, and total phosphorus in the wastewater contributed to variation in the structure and composition of families related to the sulfur cycle.…”

Performance of Anaerobic Digestion of Acidified Palm Oil Mill Effluent under Various Organic Loading Rates and Temperatures

“…Figure 7 depicts the granular structure of thermophilic and mesophilic methanogens via scanning electronic microscopy (SEM), which is suitable to determine the morphology of the microbial species in anaerobic digestion [52]. For each sample, the images are presented with 15,000× magnification that permits visualization of different types of sludge developed in the reactors and detailed morphological features of the microbial species.…”