Microbiota of gingivitis in man

Abstract

– A Study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. Samples were obtained from nine individuals 25–42 years of age. Gram‐positive rods made up 26.1% of the isolates and included mainly Actinomyces naeslundii, Actinomyces israelii, and Actinomyces viscosus. Streptococcus mitis and Streptococcus sanguis together made up 26.8% of the cultivable organisms. Feptostreptococcus averaged 3.0% of the organisms recovered. Gram‐negative anaerobic rods constituted 25.0% of the total isolates with Fusobacterium nucleatum, Bacteroides melaninogenicus ss. intermedius, Bacteroides ochraceus, other Bacteroides species, Selenomonas sputigena, and Campylobacter sputorum as the most predominant isolates. Haemophilus parainfiuenzae averaged about 14% and Veillonella species 4.3% of the cultivable microflora. The data presented indicate that the subgingival microflora of a chronic gingivitis differs from those of healthy periodontium and advanced adult and juvenile periodontitis. This might suggest that different infectious processes may be operative in various clinical entities of periodontal disease.

The Effects of Triclosan and Zinc Citrate, Alone and in Combination, on a Community of Oral Bacteria Grown in vitro

“…Products containing these agents have been shown to have anti-gingivitis properties (Saxton and van der Ouderaa, 1989;Stephen et al, 1990;Svatun et al, 1990;Gjermo and Saxton, 1991) and to reduce the selection of obligately anaerobic bacteria during an experimental gingivitis study . The data presented here support these findings by showing that combinations of zinc citrate and Triclosan are particularly inhibitory to the Gram-negative species that increase in proportion in plaque during the development of gingivitis (Slots et al, 1978;Savitt and Socransky, 1984;Moore et al, 1987). In particular, F. nucleatum and P. intermedia have been reported to be among the Gramnegative species that increase significantly in gingivitis, and these were some ofthe most sensitive species in both the dosing and pulsing studies.…”

Treatment Outcomes of Dental Flossing in Twins: Molecular Analysis of the Interproximal Microflora

“…Both of these bacteria were reported to be associated with chronic gingivitis in adults. 13,14 The initiation of gingivitis by maturing dental plaque is characterized by a proportional increase in Actinomyces sp. relative to Streptococcus sp.…”

Anaerobic culture to detect periodontal and caries pathogens

“…There is a vast literature in periodontal microbiology based on these species. Other studies using anaerobic microbiology to characterize periodontitis that improve our understanding of the cultivable microbiota, include those of Slots [40–42], Zambon [43], and most notably those of Holdeman and Moore [14]. Further culture of the related infections, pericoronitis [44], dentoalveolar abscesses [45] and endodontic infections [46] Wade’s group expand periodontal species and their role in oral infections.…”