Microbiota of <i>Telenomus tridentatus</i> (Platygastroidea: Scelionidae): An unwanted parasitoid

Ramírez‐Ahuja, María de Lourdes; Gómez-Govea, Mayra A.; Lugo‐Trampe, Ángel; Borrego‐Soto, Gissela; Delgado‐Enciso, Iván; Ponce‐García, Gustavo; Martínez‐Fierro, Margarita L.; Ramírez-Valles, Eda Guadalupe; Treviño, Víctor; Flores‐Suarez, Adriana E.; Rodríguez‐Sánchez, Iram P.

doi:10.1111/jen.12656

Cited by 6 publications

(3 citation statements)

References 65 publications

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“…Additionally, relative abundance of Staphylococcus was above 0.5% only in one replicate out of three (Table S5) and it did not appear in the MR microbiome. This bacterium has been repeatedly reported as a gut microbe in insects (e.g., wasp, 47 parasitoid wasp, 43 aphids and moths, 48 Beetles, 49 termites 50 ). No substantial association between arthropods and Carnimonas has been previously reported (although it was found in surveys of surroundings of honeybees 51,52 ), and since our attempts to detect it by diagnostic PCRs have failed, we concluded that it was a single case of contamination or a ‘by‐ passer’.…”

Section: Discussionmentioning

confidence: 87%

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Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

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BACKGROUND Like numerous other animals, biocontrol agents (BCAs) of arthropod pests carry various microorganisms that may have diverse effects on the biology of their eukaryote hosts. We postulated that it is possible to improve the efficacy of BCAs by manipulating the composition of their associated microbiota. The parasitoid wasp Anagyrus vladimiri (Hymenoptera: Encyrtidae) from a mass‐rearing facility was chosen for testing this hypothesis. RESULTS High‐throughput sequencing analysis indicated that fungal abundance in A. vladimiri was low and variable, whereas the bacterial community was dominated by the endosymbiont Wolbachia. Wolbachia was fixed in the mass‐rearing population, whereas in field‐collected A. vladimiri Wolbachia's prevalence was only approximately 20%. Identification of Wolbachia strains from the two populations by Multi Locus Sequence Typing, revealed two closely related but unique strains. A series of bioassays with the mass‐rearing Wolbachia‐fixed (W+) and a derived antibiotic‐treated Wolbachia‐free (W–) lines revealed that: (i) Wolbachia does not induce reproductive manipulations; (ii) W– females have higher fecundity when reared individually, but not when reared with conspecifics; (iii) W+ females outcompete W– when they share hosts for oviposition; (iv) longevity and developmental time were similar in both lines. CONCLUSIONS The findings suggest that W+ A. vladimiri have no clear fitness benefit under mass‐rearing conditions and may be disadvantageous under lab‐controlled conditions. In a broader view, the results suggest that augmentative biological control can benefit from manipulation of the microbiome of natural enemies.

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Section: Discussionmentioning

confidence: 87%

“…Only a handful of papers reported the characterization of parasitoids' microbiome by molecular methods 43–46 . In those, a diverse community of bacteria was found, most of which was attributed to environmental microbes, associated with gut activity or transient.…”

Section: Discussionmentioning

confidence: 99%

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

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“…Studies of bacterial diversity in Nasonia have shown the presence of Proteobacteria (74.4%), Actinomyces (15.7%), and Firmicutes (9.5%) [10]. Ramírez-Ahuja et al [28] reported the microbiomes of Telenomus tridentatus (Platygastroidea: Scelionidae) (Proteobacteria, Actinobacteria, and Firmicutes), which were similar to the results obtained at the phylum level.…”

Section: Discussionsupporting

confidence: 62%

Unveiling the Microbiome Diversity in Telenomus (Hymenoptera: Scelionidae) Parasitoid Wasps

Gómez-Govea,

Peña-Carillo,

Ruiz-Ayma

et al. 2024

Insects

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Bacterial symbionts in insects constitute a key factor for the survival of the host due to the benefits they provide. Parasitoid wasps are closely associated with viruses, bacteria, and fungi. However, the primary symbionts and their functions are not yet known. This study was undertaken to determine the gut microbiota of six species of the Telenomus genus: T. alecto (Crawford), T. sulculus Johnson, T. fariai Costa Lima, T. remus Nixon, T. podisi Ashmead, and T. lobatus Johnson & Bin. Wasp parasitoids were collected from their hosts in different locations in Mexico. DNA was extracted from gut collection, and sequencing of bacterial 16S rRNA was carried out in Illumina® MiSeq™. Among the six species of wasps, results showed that the most abundant phylum were Proteobacteria (82.3%), Actinobacteria (8.1%), and Firmicutes (7.8%). The most important genera were Delftia and Enterobacter. Seventeen bacteria species were found to be shared among the six species of wasps. The associate microbiota will help to understand the physiology of Telenomus to promote the use of these wasp parasitoids in the management of insect pests and as potential biomarkers to target new strategies to control pests.

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Characterization of larval gut microbiota of two endoparasitoid wasps associated with their common host, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae)

Hu,

Wang,

Zhang

et al. 2024

Microbiol Spectr

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Insect gut microbes play important roles in digestion, metabolism, development, and environmental adaptation. Parasitoid wasps are one of the most important biological control agents in pest control, while the gut microbial species compositions and the associated functions have been poorly investigated. Two endoparasitoid wasps, Cotesia vestalis and Diadromus collaris , parasitize the larval stage and pupal stage of the diamondback moth, Plutella xylostella , respectively. Using whole-genome shotgun metagenomic sequencing, we characterized the gut microbial composition, diversity, and potential functional roles associated with the two parasitoid wasp larvae. The results reveal that Proteobacteria and Firmicutes are the dominant phyla in the gut of C. vestalis and D. collaris larvae, with Rhizobium and Enterococcus being the dominant genera. The putative microbial functions associated with the two parasitoid wasps might play a virtual role in assisting in consuming the host’s nutritional composition. The enriched CAZymes family genes are primarily involved in the degradation and synthesis of chitin. Despite the richness of microbial species and communities, the microbes species and the microbial community structure exhibit significant similarity between the two parasitoid wasps and between the parasitoid wasp and the host P. xylostella . Notably, the prevalence of the genus Enterococcus shared among them suggests a possible link of gut microbes between the host and their associated parasitoids. Our study offers insights into the gut microbe-based interactions between the host and parasitoid wasps for the first time, potentially paving the way for the development of an ecologically friendly biocontrol strategy against the pest P. xylostella . IMPORTANCE Endoparasitoid wasps spend the majority of their lifespan within their host and heavily rely on the host’s nutrition for survival. There is limited understanding regarding the composition and physiological impacts of gut microbial communities in parasitoid wasps, particularly during the larval stage, which is directly linked to the host. Based on a thorough characterization of the gut microbe and comprehensive comparative analysis, we found the microbial species of the larval parasitoid wasp Cotesia vestalis and the pupal parasitoid wasp Diadromus collaris were similar, sharing 159 genera and 277 species, as were the microbial community structure. Certain of the dominant microbial strains of the two parasitoid wasps were similar to that of their host Plutella xylostella larvae, revealing host insect may affect the microbial community of the parasitoid wasps. The putative microbial functions associated with the parasitoid wasp larvae play an important role in dietary consumption.

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Microbiota of Telenomus tridentatus (Platygastroidea: Scelionidae): An unwanted parasitoid

Cited by 6 publications

References 65 publications

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Unveiling the Microbiome Diversity in Telenomus (Hymenoptera: Scelionidae) Parasitoid Wasps

Characterization of larval gut microbiota of two endoparasitoid wasps associated with their common host, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae)

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