Microbiota state of the gastro-intestinal tract of laboratory animals with signs of metabolic dysbiosis during treatment with experimental medical drug

Abstract

Objective. The work was performed with the purpose to study microbiota state of the gastro-intestinal tract (GIT) of C57Bl/6 mice with signs of metabolic dysbiosis (MD) induced with the use of fructose solution or with poloxamer 407 (P 407), during treatment with combination of sodium polyprenylphosphate (PPP) and beta-sitosterol (BSS). Materials and Methods. MD was induced in mice with drinking 20 per cent water fructose solution. MD was also induced in mice by intraperitoneal use of P 407 at the dose of 500 mg/kg two times per week during ten weeks. The mice of experimental groups received intragastrically combination of PPP and BSS preparations (0,2 ml of the suspension per mouse). Medical scheme consisted in use of preparations five times per week starting after one month from the first day of fructose drinking or of P 407 use and prolonging for one month else. The quantity of viable bacteria in the probes obtained was measured by the direct passage of the material tested on elective or selective nutritional media with following count of colonies grown. Results. The drink of fructose solution or use of P 407 caused the changes of GIT microbiota state that confirmed development of signs of MD in mice. Treatment with PPP and BSS combination was shown to cause actual tendencies to restore the normal GIT microbiota state. Conclusion. Combination of PPP and BSS preparations seemed to be efficient as a novel approach to correction of GIT microbiota state perturbed due to pathogenesis of MD considered as one of the primary signs of metabolic syndrome in mice.