Microbore liquid chromatography with dual electrochemical detection for the determination of serotonin and 5-hydroxyindoleacetic acid in rat brain dialysates

Sarre, Sophie; Michotte, Yvette; Marvin, Craig A.; Ebinger, Guy

doi:10.1016/0378-4347(92)80298-5

Cited by 28 publications

(7 citation statements)

References 10 publications

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“…The use of microbore LC is, in our opinion, the key factor for measuring this transmitter with more ease. This has already been demonstrated for the determination of 5HT in hippocampus and striatum, where we can measure extracellular 5HT without the need of addition of a re-uptake inhibitor to the perfusion fluid (Sarre et al, 1992b;Thorr6 et al, 1996). Using microbore LC we were also able to measure hippocampal DA easily.…”

Section: Discussionmentioning

confidence: 87%

“…The dialysates from the L-5HTP experiments were analysed for 5HT and 5HIAA without sample preparation, using a microbore LC system combined with dual electrochemical detection as described before (Sarre et al, 1992b). Briefly, this system consisted of an LC pump (Gilson, Villiers-le-Bel, France) operating at a flow rate of 0.8ml/min and connected to a flow splitter kit (Bioanalytical Systems, U.S.A.) to obtain a microflow of 66 ~tl/ rain across a Unijet| microbore column: 100 • 1ram i.d., C18 3~am (Bioanalytical Systems, U.S.A.).…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

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Biotransformation of locally applied precursors of dopamine, serotonin and noradrenaline in striatum and hippocampus: A microdialysis study

Sarre

Smolders

Thorré

et al. 1997

J. Neural Transmission

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In vivo microdialysis in freely moving rats was used to study the biotransformation, consisting primarily of decarboxylation by aromatic amino acid decarboxylase (AAAD), of the precursors L-3,4-dihydroxyphenylalanine (L-DOPA), L-5-hydroxytryptophan (L-5HTP), and L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) on extracellular levels of dopamine (DA), serotonin (5HT) and noradrenaline (NA), respectively. The precursors were administered locally through the microdialysis probe into the striatum and into the hippocampus. The different transmitter systems were compared with respect to the ability of the precursors to elevate extracellular levels of their associated transmitter. The basal extracellular concentrations of NA and DA were found to be tetrodotoxin (TTX, a blocker of fast sodium channels) sensitive in striatum and hippocampus, indicating the neuronal origin of the measured transmitters. The extracellular concentrations of 5HT (in hippocampus) were only 60% TTX-sensitive. L-DOPA and L-5HTP showed to be effective precursors of DA and 5HT, respectively, although their formation profile was quite different. The L-DOPA-induced increase in extracellular DA was large and short-lasting, while the L-5HTP-induced increase in 5HT was slower and less pronounced. The relative increase in extracellular DA or 5HT was more pronounced in the brain region where their baseline values were lower, but the absolute amount of transmitter formed from their precursor was similar in both brain regions. L-threo-DOPS was a poor precursor for NA and also failed to influence extracellular DA in striatum, questioning its use in the treatment of freezing gait in late stages of Parkinson's disease.

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Section: Discussionmentioning

confidence: 87%

Section: Chromatographic Conditionsmentioning

confidence: 99%

Biotransformation of locally applied precursors of dopamine, serotonin and noradrenaline in striatum and hippocampus: A microdialysis study

Sarre

Smolders

Thorré

et al. 1997

J. Neural Transmission

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“…The detection of serotonin and its metabolites in rat brain dialysate [186], the analysis of terbutaline in human plasma [187], and the detection of biogenic amines in brain tissue [188] are just a few examples. A more detailed description about the use of electrochemical detection in bioanalysis will be given in Section 7.…”

Section: Electrochemical Detectionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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“…Neurotransmitters diffuse from the extracellular fluid to the aCSF inside the probe through the microdialysis membrane and are analyzed in the collected samples. Microdialysate 5-HT has been classically analyzed by column liquid chromatography (LC) with electrochemical detection [4,5], and LC with miniaturization of column dimensions and instrumentation has resulted in an improved limit of detection [6]. LC with post-column or pre-column derivatization with benzylamine/K 1 hexacyanoferrate and fluorescence detection has been proposed as another approach for assaying 5-HT in microdialysates [7,8].…”

Section: Introductionmentioning

confidence: 99%

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

et al. 2005

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Serotonin or 5-hydroxytryptamine (5-HT) is a major neurotransmitter in the central nervous system. In this work, a method for analyzing 5-HT in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. A pH-mediated in-capillary preconcentration of samples was performed, and after separation by capillary zone electrophoresis, native fluorescence of 5-HT was detected by a 266 nm solid-state laser. The separation conditions for the analysis of 5-HT in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical bases. Separation of 5-HT was performed using a 80 mmol/L citrate buffer, pH 2.5, containing 20 mmol/L hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and +30 kV voltage. The detection limit was 2.5 x 10(-10) mol/L. This method allows the in vivo brain monitoring of 5-HT using a simple, accurate CE measurement in underivatized microdialysis samples.

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Microbore liquid chromatography with dual electrochemical detection for the determination of serotonin and 5-hydroxyindoleacetic acid in rat brain dialysates

Cited by 28 publications

References 10 publications

Biotransformation of locally applied precursors of dopamine, serotonin and noradrenaline in striatum and hippocampus: A microdialysis study

Biotransformation of locally applied precursors of dopamine, serotonin and noradrenaline in striatum and hippocampus: A microdialysis study

Microcolumn liquid chromatography: instrumentation, detection and applications

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

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