Microcalorimetric investigations of the metabolism of yeasts VII. Flow-calorimetry of aerobic batch cultures

Brettel, R.; Lamprecht, I.; Schaarschmidt, B.

doi:10.1007/bf01324273

Cited by 27 publications

(8 citation statements)

References 9 publications

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“…For glucose-grown cells, the specific activity of GPDH was ca. 20 mU/mg of protein during the respirofermentative phase ( 6.0 throughout growth, the overall heat production per biomass formation for respiratory-phase cells was 30.2 J/mg (dry weight) formed (6). This is in accordance with the value of dQ/dX for E/R1 ( Table 2).…”

Section: Resultssupporting

confidence: 84%

“…1), which explains the decrease in fermentation. Glucose was exhausted between G/T and G/Rl, in contradiction to the results of Brettel et al (6), who reported glucose to be exhausted at the apex in the heat production rate curve prior to G/T. The respiratory activity is subject to glucose repression (12) and was not fully expressed until point G/R2 (Fig.…”

Section: Resultsmentioning

confidence: 55%

“…It should be pointed out that during batch growth, the physiological state of the culture will often be composed of a mixture of different metabolic states. A high degree of resolution of the growth process can be achieved by measuring the heat production rate (dQ/dt), which enables observations of fine temporal events during growth (6,25).…”

Section: (23)mentioning

confidence: 99%

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Microcalorimetric monitoring of growth of Saccharomyces cerevisiae: osmotolerance in relation to physiological state

1988

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The importance of the physiological state of a culture of Saccharomyces cerevisiae for tolerance to sudden osmotic dehydration was studied, and it was investigated whether specific osmotolerance factors were demonstrable. The microcalorimeter was used to monitor growth, and different physiological states of the culture were selected and their osmotolerance was tested. In addition to cells in the stationary phase, cells from the transition phase between respirofermentative and respiratory catabolism were osmotolerant. S. cerevisiae exhibited ever-changing metabolism during batch growth on either glucose or ethanol as the carbon source. Instantaneous heat production per biomass formation (dQ/dX) and specific activity of sn-glycerol 3-phosphate dehydrogenase (GPDH) (EC 1.1.1.8) were shown to differ for different physiological states. Neither high respiratory activity nor low total cellular activity, nor factors involved in osmoregulation, i.e., intracellular glycerol or activity of GPDH, correlated with the osmotolerant phenotype.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 55%

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Microcalorimetric monitoring of growth of Saccharomyces cerevisiae: osmotolerance in relation to physiological state

1988

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“…Brettel et al reported that the metabolic and growth rate of S. cerevisiae remained almost constant in dextrose levels between 0.16 to 1.6% [42]. S. cerevisiae does switch to respiratory metabolism when the medium is depleted of dextrose, but it appears that the cells must actually be starved of dextrose before they undergo a diauxic shift [12].…”

Section: Discussionmentioning

confidence: 99%

Robust Metabolic Responses to Varied Carbon Sources in Natural and Laboratory Strains of Saccharomyces cerevisiae

Voorhies¹

2012

PLoS ONE

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Understanding factors that regulate the metabolism and growth of an organism is of fundamental biologic interest. This study compared the influence of two different carbon substrates, dextrose and galactose, on the metabolic and growth rates of the yeast Saccharomyces cerevisiae. Yeast metabolic and growth rates varied widely depending on the metabolic substrate supplied. The metabolic and growth rates of a yeast strain maintained under long-term laboratory conditions was compared to strain isolated from natural condition when grown on different substrates. Previous studies had determined that there are numerous genetic differences between these two strains. However, the overall metabolic and growth rates of a wild isolate of yeast was very similar to that of a strain that had been maintained under laboratory conditions for many decades. This indicates that, at in least this case, metabolism and growth appear to be well buffered against genetic differences. Metabolic rate and cell number did not co-vary in a simple linear manner. When grown in either dextrose or galactose, both strains showed a growth pattern in which the number of cells continued to increase well after the metabolic rate began a sharp decline. Previous studied have reported that O2 consumption in S. cerevisiae grown in reduced dextrose levels were elevated compared to higher levels. Low dextrose levels have been proposed to induce caloric restriction and increase life span in yeast. However, there was no evidence that reduced levels of dextrose increased metabolic rates, measured by either O2 consumption or CO2 production, in the strains used in this study.

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“…This heat represents the free energy dissipated irreversibly during metabolism, providing an all-inclusive measure of metabolic activity. We built up on previous studies on yeast microcalorimetry [7][10] [11], and by applying state of the art electronics for data acquisition and analysis, highly accurate reproductive data were obtained with our flow microcalorimetry set-up. As opposed to previous studies in which prototrophic strains were used [7] [6], we analyze an auxotrophic strain (BY4741) that is commonly used in laboratory studies [12].…”

Section: Introductionmentioning

confidence: 99%

The metabolic profile of lag and exponential phases ofSaccharomyces cerevisiaegrowth changes continuously

Bento¹,

Var³

et al. 2016

Preprint

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Cellular growth is usually separated in well-defined phases. For microorganism like Saccharomyces cerevisiae, two phases usually defined are (1) a lag phase, in which no growth is observed and cells adapt to a new environment, followed by (2) an exponential phase, in which rapid proliferation occurs. Here we investigate whether these well-defined phases are uniform. By using flow-microcalorimetry, we found that the metabolic profile of the culture is continuously changing, both in the lag and exponential phases of growth. Along the lag phase there is a continuous increase in the energy that is dissipated irreversibly as heat, while in the exponential phase the opposite occurs. We also confirm recent observations that the oxidative component of metabolism decreases along the exponential phase. Interestingly, nutrient limitation further decreases the amount of energy that is dissipated irreversibly. Altogether, this points to a picture in which cells respond rapidly to minute environmental changes by adjusting their metabolic profile.

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Microcalorimetric investigations of the metabolism of yeasts VII. Flow-calorimetry of aerobic batch cultures

Cited by 27 publications

References 9 publications

Microcalorimetric monitoring of growth of Saccharomyces cerevisiae: osmotolerance in relation to physiological state

Microcalorimetric monitoring of growth of Saccharomyces cerevisiae: osmotolerance in relation to physiological state

Robust Metabolic Responses to Varied Carbon Sources in Natural and Laboratory Strains of Saccharomyces cerevisiae

The metabolic profile of lag and exponential phases ofSaccharomyces cerevisiaegrowth changes continuously

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