Microcalorimetric Study of Energy Metabolism of HSV‐2 and FMDV Infection Processes

Abstract

The metabolic thermogenic power data of the HSV-2 infected HeLa cells and the FMDV infected BHK-21 cells were determined by LKB-2277 bioactivity monitor. The aim of the study was to investigate the difference of the cell metabolism under the action of two different viruses and the effects of hyperthermia and drugs on it. The results illustrated that the metabolic thermogenic power of infected cells was larger than the uninfected ones and there was a significant difference between the metabolism heat released by the two types of infected cells. From the maximal thermal power and total metabolism heat, the infection process was observed to be thermosensitive and could be inhibited by interferon. Our experiments also revealed that 6 month storage of FMDV could attenuate its virulence and infectivity. The study shows that microcalorimetry is a potent tool to investigate the metabolism of virus infection process.

Measurement and correlation of the solubility of 3,4-bis(3-nitrofurazan-4-yl)furoxan (DNTF) in different solvents

“…Further study shows that its comprehensive property is more superior than octa hydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) but close to 1, 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) [1]. From the study of the crystal structure of DNTF by Zhou et al [2], we can conclude that the three five-membered rings of DNTF are not coplanar and they form a chair structure in three-dimensional space.…”