Microcalorimetric study of the growth of bacterial colonies of Lactococcus lactis IL1403 in agar gels

Kabanova, Natalja; Stulova, Irina; Vilu, Raivo

doi:10.1016/j.fm.2011.08.018

Cited by 50 publications

(38 citation statements)

References 46 publications

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“…In solid medium or in solid substrates, the peaks in the thermal power curve (i.e., the metabolic activity) tend to become smaller but broader with decreasing inoculum (or bacterial density). This has been observed in agar medium, fermented milk products, and speleothems (i.e., secondary mineral deposit formed in a cave) for example [67][68][69]. This phenomenon can easily be explained by the limitation in substrates availability introduced by the medium.…”

Section: Special Casesmentioning

confidence: 89%

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Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Braissant

Bachmann

Bonkat

2015

Methods

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Section: Special Casesmentioning

confidence: 89%

“…Especially, when opaque, solid, semi-solid or encapsulated samples are involved [68,121,122]. In food microbiology several fermentation processes take place in opaque or solid matrices such as milk or dough.…”

Section: Application Of Isothermal Microcalorimetry In Other Fieldsmentioning

confidence: 99%

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Braissant

Bachmann

Bonkat

2015

Methods

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“…Using the Y Q value 2.58 ± 0.44 × 10 -9 J/cfu determined by us for L. lactis IL1403 [10] the average number of bacteria grown during the exponential growth (N exp , cfu/g-5.93 × 10 9 cfu/g at neutral pH and 5.29 × 10 9 cfu/g at low pH) was calculated-see Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…The construction and functioning of the TAM III calorimeter were described earlier by Wadsö [8]. Microcalorimetric power-time curves were analyzed as described by Kabanova [10]. The calorimetric trials were performed at fixed temperature of incubation at 37˚C (equivalent of dogs body temperature [20]) using 3 mL calorimetric ampoules.…”

Section: Microcalorimetrymentioning

confidence: 99%

“…It has been shown that generation of heat by microbial cultures can be used for on-line monitoring of growth and metabolism of cells [4][5][6][7][8][9][10]. Very sensitive measurements of heat flows using microcalorimetry is one of the most attractive techniques for studying and monitoring of the metabolic activity of bacteria in opaque liquid media, and solid matrices [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

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Microcalorimetric Study of Extruded Dog Food Containing Probiotic Micro-Organisms

Kazarjan¹,

Kabanova²,

Vilu³

2012

AiM

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Extruded dry dog food products claimed to have a probiotic ingredient of Enterococcus faecium (NCIMB10415) in the commercial available formulations under the brand name of ProBiotic LIVE (Bacterfield S.A., Luxembourg) were studied in the present work using a multichannel thermal activity monitor TAM III. Maximum specific growth rate, heat produced during different growth phases, and lag-phase duration were determined. The length of the lag-phase that can be used to determine the time necessary for the probiotic ingredient to restore its activity after consumption of probiotic containing extruded products was also measured. The calorimetric data confirmed the ability of the Enterococcus faecium to grow at the acidic pH conditions, modeling conditions of gastro-intestinal tract of dogs, and preserve its metabolic activity (viability) at the same level as at the neutral pH. The results obtained indicated that microcalorimetry was a precise and convenient tool for monitoring probiotic activity in complicated solid-state matrices.

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Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label‐free well‐plate assay

Braissant

Keiser

Meister

et al. 2015

Biotechnology Journal

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Isothermal microcalorimetry is a label-free assay that allows monitoring of enzymatic and metabolic activities. The technique has strengths, but most instruments have a low throughput, which has limited their use for bioassays. Here, an isothermal microcalorimeter, equipped with a vessel holder similar to a 48-well plate, was used. The increased throughput of this microcalorimeter makes it valuable for biomedical and pharmaceutical applications. Our results show that the sensitivity of the instrument allows the detection of 3 × 104 bacteria per vial. Growth of P. mirabilis in Luria Broth medium was detected between 2 and 9 h with decreasing inoculum. The culture released 2.1J with a maximum thermal power of 76 μW. The growth rate calculated using calorimetric and spectrophotometric data were 0.60 and 0.57 h–1, respectively. Additional insight on protease activities of P. mirabilis matching the last peak in heat production could be gathered as well. Growth of tumor microtissues releasing a maximum thermal power of 2.1 μW was also monitored and corresponds to a diameter increase of the microtissues from ca. 100 to 428 μm. This opens new research avenues in cancer research, diagnostics, and development of new antitumor drugs. For parasitic worms, the technique allows assessment of parasite survival using motor and metabolic activities even with a single worm.

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Microcalorimetric study of the growth of bacterial colonies of Lactococcus lactis IL1403 in agar gels

Cited by 50 publications

References 46 publications

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Microcalorimetric Study of Extruded Dog Food Containing Probiotic Micro-Organisms

Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label‐free well‐plate assay

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