Microcapsules Preparation of Alcohol Extracts from Nymphaea Hybrid and Determination of Its Antioxidant Capability in vitro and in vivo

Abstract

To find efficient natural antioxidants and further improve the utilization value of Nymphaea hybrid, the alcohol extracts from that are embedded and an all-round evaluation for the antioxidant activity is carried out. The microcapsule process is optimized by Box-Behnken response surface analysis which includes the wall-to-core ratio of 3.39:1, inlet air temperature at 195.56 °C, and the homogeneous number of 4.03, exhibiting the highest microencapsulation efficiency (94.36%). Antioxidant experiments in vitro showed that there was no significant difference between alcohol extracts and microcapsule on DPPH•, •O 2 and •OH, while the scavenging activity of microcapsule on ABTS + • was lower against alcohol extracts. It was worth noting that the activities of GSH, SOD and CAT in vivo were increased about 55.55%, 29.13% and 51.23%. Similar results were obtained with microcapsule treatment. The heat treatment results showed the scavenging activities of alcohol extracts and microcapsule on antioxidant experiments in vitro were all decreased; however, the antioxidant activity of microcapsule was higher than that of alcohol extracts, indicating that the microencapsulation could improve the thermal stability. In conclusion, Nymphaea hybrid alcohol extracts is a well natural antioxidants, moreover, this study provide basic support for comprehensive utilization of Nymphaea hybrid.

Preparation of Dendrobium officinale Flower Anthocyanin and Extended Lifespan in Caenorhabditis elegans

“…When the addition amount of ACNs was 180 μL (approximately 0.3 mg/mL), the clearance effect on DPPH radical was the best, up to 96.78 ± 2.14%, while the DPPH· clearance of 60 μL (approximately 0.1 mg/mL) was 81.98 ± 2.71% ( Figure 3 A). In our previous study [ 28 ], the clearance effect of 0.1 mg/mL Nymphaea hybrid alcohol extracts and vitamin C on DPPH· radical was 41.71 ± 1.42% and 55.15 ± 2.21%, respectively. These results show that the activity of ACNs in scavenging DPPH· free radicals was higher than that of vitamin C. For the scavenging of ABTS + · free radicals, the clearance increased with the increasing ACN dose (within 80 μL).…”

Preparation, characterization and antioxidant activity of polysaccharides copper from Qingzhuan dark tea

“…The absorbance was determined at 510 nm (A 1 ). According to aforementioned operation, the solution without sample was taken as A 0 , and the clearance rate was calculated as (Equation 1) (Hang et al, 2022).…”

Extraction Optimization, Characterization and Biological Activities of Polysaccharide Extracts from Nymphaea hybrid

“…In the theory of dermatology, ultraviolet (UV) stimulation, inflammatory stimulation, viruses and bacteria can increase the level of ROS free radicals, create oxidative stress and even cause injuries to skin cells, such as cell aging, necrosis and apoptosis. In recent studies, the alcohol extracts (mainly flavonoids and polyphenols) from NH have been proven to have antioxidant activity, including strong scavenging activities on free radical and lipid peroxidation inhibition ability, indicating to some extent that the alcohol extract from NH can act as anti-aging active material in the field of cosmetics [4,5]. Moreover, various extracts of NH are also used in various fields such as food [3,6] and medicine [4,7].…”

“…In recent studies, the alcohol extracts (mainly flavonoids and polyphenols) from NH have been proven to have antioxidant activity, including strong scavenging activities on free radical and lipid peroxidation inhibition ability, indicating to some extent that the alcohol extract from NH can act as anti-aging active material in the field of cosmetics [4,5]. Moreover, various extracts of NH are also used in various fields such as food [3,6] and medicine [4,7]. However, most of the research on NH has been focused on alcohol extracts, which often face solubility, stability and other problems for application in the aqueous systems.…”