Fabrication and analysis of microchannels on glass (coverslip) substrate are presented here. Microchannels are fabricated by using electrochemical spark micromachining process developed in house. The said process is evolved for machining of non-metals. The paper discusses in brief, the design and development of the experimental set-up along with the technique devised for the analysis of fabricated microchannels in terms of their shape, surface topography, and dimensions. The technique is based on making the negative replica of the microchannel by polydimethysiloxane (PDMS).