Microchip-based ELISA strategy for the detection of low-level disease biomarker in serum

Liu, Yun; Wang, Huixiang; Huang, Jingyu; Yang, Jie; Liu, Baohong; Yang, Pengyuan

doi:10.1016/j.aca.2009.06.048

Cited by 81 publications

(57 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For this challenge, electrical transduction methods are more suitable for the detection of ionic species (oxidable or reducable) because of their high sensitivity and no need labelling step is required [4][5][6]. The relevance of electrochemical detection on microchips with improvement of micro nanoelectrode fabrication [7][8][9][10][11][12] has been widely proven, including the application of amperometric detection [13][14][15][16], and potentiometric [17][18][19]. However, contactless to the microelectrodes as electrical detection scheme with the electrolyte is the most promising [20][21][22][23].…”

Section: Introductionmentioning

confidence: 98%

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Kechadi

Sotta

Gamby

2015

Talanta

View full text Add to dashboard Cite

This paper presents the use of polymer coated microelectrodes for the realtime conductivity monitoring in a microchannel photoablated through the polymer without contact. Based on this strategy, a small conductometry sensor has been developed to record in time conductivity variation when an enzymatic reaction occurs through the channel. The rate constant determination, k2, for the dephosphorylation of organic phosphate-alkaline phosphatase-superparamagnetic beads complex using chemically different substrates such as adenosine monoesterphosphate, adenosine diphosphate and adenosine triphosphate was taken as an example to demonstrate selectivity and sensivity of the detection scheme. The k2 value measured for each adenosine phosphate decreases from 39 to 30 s(-1) in proportion with the number (3, 2 and 1) of attached phosphate moiety, thus emphasizing the steric hindrance effect on kinetics.

show abstract

Section: Introductionmentioning

confidence: 98%

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Kechadi

Sotta

Gamby

2015

Talanta

View full text Add to dashboard Cite

show abstract

“…Recently, some electrochemical microfluidic immunosensors also have been developed for many analytical applications such as for cancer biomarkers [13][14][15]. Besides, in the last years, different nanomaterials have been incorporated to electrochemical sensors as platform of the specific immunoreactants, which through an appropriate immobilization process enable the fabrication of a biorecognition layer with desirable properties (i.e., large loading, well-preserved bioactivity and good reversibility) [16].…”

Section: Introductionmentioning

confidence: 99%

Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform

Ortega

Fernández‐Baldo

Serrano

et al. 2015

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…The ability to detect and identify low levels of analytes is of growing importance in medicine and nanotechnology [1][2][3][4][5][6][7]. Diagnostic monitoring of drug delivery nanoparticle levels [8][9][10], identification of cell-free circulating (cfc) DNA/RNA and other nanoparticulate biomarkers [11][12][13][14][15], and detection of pathogens in clinical and environmental samples [16] are examples where low level detection of analytes are of extreme importance.…”

Section: Introductionmentioning

confidence: 99%

Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays

McCanna¹,

Sonnenberg²,

Heller³

2013

Journal of Biophotonics

View full text Add to dashboard Cite

Common epifluorescent microscopy lacks the sensitivity to detect low levels of analytes directly in clinical samples, such as drug delivery nanoparticles or disease related DNA biomarkers. Advanced systems such as confocal microscopes may improve detection, but several factors limit their applications. This study now demonstrates that combining an epifluorescent microscope with a dielectrophoretic (DEP) microelectrode array device enables the detection of nanoparticles and DNA biomarkers at clinically relevant levels. Using DEP microarray devices, nanoparticles and DNA biomarkers are rapidly isolated and concentrated onto specific microscopic locations where they are easily detected by epifluorescent microscopy. In this study, 40nm nanoparticles were detected down to 2–3 × 103/ul levels and DNA was detected down to the 200 pg/ml level. The synergy of epifluorescent microscopy and DEP microarray devices provides a new paradigm for DNA biomarker diagnostics and the monitoring of drug delivery nanoparticle concentrations. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

Microchip-based ELISA strategy for the detection of low-level disease biomarker in serum

Cited by 81 publications

References 25 publications

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Microchannel conductivity measurements in microchip for on line monitoring of dephosphorylation rates of organic phosphates using paramagnetic-beads linked alkaline phosphatase

Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platform

Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays

Contact Info

Product

Resources

About