Microchip coupled with MALDI-TOF MS for the investigation of bacterial contamination of fish muscle products

Algorithms push forward the application of MALDI–TOF mass fingerprinting in rapid precise diagnosis

“…Nowadays, along with the improvement of microbial enrichment techniques and database searching algorithms, accurate identification has been achieved for microbial mixtures and complex samples directly. Strategies like differential centrifugation, 61 affinity isolation, 62 magnetic precipitation, 63 and microchip separation 64 have been used to enrich and isolate microbes from patient or environmental specimens, either directly or after a rough culture. The isolated products, containing either single or multiple microbial species, are then directly subjected to mass fingerprinting for species identification.…”

Isothermal Amplification and CRISPR/Cas12a-System-Based Assay for Rapid, Sensitive and Visual Detection of Staphylococcus aureus

“…Traditional detection methods, such as culture-based counting for colony-forming units, heterotrophic plate counts, the spectrophotometer method of optical density measurement and flow cytometry, are simple but need bacteria enrichment and to prolong detection time [7]. Immunoassay methods, for instance, the enzyme-linked immunosorbent assay, enzyme-linked fluoroimmunoassay and immunomagnetic bead separation, are easy to automate but have a degree of complicated operation and long incubation time [8][9][10].…”

Microfluidic Electrochemical Integrated Sensor for Efficient and Sensitive Detection of Candida albicans

“…are also applicable for microbial detection, they always require expensive experimental instruments or specialized techniques, which are not conducive to their widespread use, especially in low-resource areas.Microchip has the features of integration, high throughput, and less reagent consumption, which are very suitable for constructing economical devices. 11−13 Currently, various sorting strategies (membrane filtration, 14 inertial microfluidic chip, 15 magnetic nanoparticle-based separation 16−18 based separation, and so on) have been integrated in the microchip to separate pathogens, which are then identified by diverse sensor systems, such as fluorescence, 19,20 colorimetry, 18,21 surface-enhanced Raman biosensors (SERS), 22,23 and mass spectrometry 24,25 . The microchips integrated with sorting and sensor systems are promising for processing complicated matrixes for the enumeration of target pathogens, but they are either complicated in microfabricating procedures or elaborate…”

“…Microchip has the features of integration, high throughput, and less reagent consumption, which are very suitable for constructing economical devices. − Currently, various sorting strategies (membrane filtration, inertial microfluidic chip, magnetic nanoparticle-based separation − based separation, and so on) have been integrated in the microchip to separate pathogens, which are then identified by diverse sensor systems, such as fluorescence, , colorimetry, , surface-enhanced Raman biosensors (SERS), , and mass spectrometry , . The microchips integrated with sorting and sensor systems are promising for processing complicated matrixes for the enumeration of target pathogens, but they are either complicated in microfabricating procedures or elaborate in manual operations, which are not realistic for batch production and application. , Some microchips may still be in lack of satisfactory performance in portability, convenience, efficient separation, and sensitive detection of targets. , Therefore, the development of a simple, low-cost, and easily manipulated microfluidic sensor for fast and highly efficient separation and detection of pathogens is still a very attractive target.…”