Microchips for detection of exfoliated tumor cells in urine for identification of bladder cancer

Liang, Liguo; Wang, Yimin; Lu, Shu‐Ling; Kong, Mengqi; Lin, Yong; Cuzzucoli, Fabio; Wang, Ping; Wang, Shuqi

doi:10.1016/j.aca.2018.07.044

Cited by 12 publications

(9 citation statements)

References 24 publications

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“…The results of this case study showed that lactate increased 600% after the fight compared to baseline, with almost full recovery within 24 hr. These data agree with other studies that report an increase in this metabolite after intense exercise, being a commonly used predictor for effort intensity measurement (Belli et al, 2018;Damas et al, 2016;Liang et al, 2018). Lactate is an intermediate of carbohydrate metabolism in anaerobic condition, as O 2 consumption does not increase instantaneously and proportionally to the initial energy demand of exercise.…”

Section: F I G U R Esupporting

confidence: 91%

“…Regarding cortisol levels, studies show that the increase of this marker in the described conditions, in simulated fight, indicates increased catabolite status (Greenham, Buckley, Garrett, Eston, & Norton, 2018;Liang et al, 2018). Cortisol is the major glucocorticoid secreted by the adrenal cortex.…”

Section: F I G U R Ementioning

confidence: 96%

“…To prepare the urine sample for microscopic analysis, a fresh sample of 10 to 15ml of urine was centrifuged at 1,500 to 2,000 rpm for 10 min (EBA 270). The method of analysis used was based on the model suggested the study by Liang et al (Liang et al, 2018).…”

Section: Urine Sampling and Analysismentioning

confidence: 99%

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Hematological parameters in champion of Brazilian jiu‐jitsu paradesport: Case study

et al. 2020

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The behavior of biochemical and immunological parameters investigated in the field conditions in athletes is important to influence in the management of recovery and disease prevention as well as, to support the training program, as well as to improve the physical conditioning associated with health and performance. However, for amputee athletes, Brazilian jiujitsu paradesport practitioners, there are no published data to date. Thus, the objective of this case study was to quantify the magnitude of biochemical, hematological, and urinary alterations after a simulated fight session in elite athlete with world titles. Outcomes were obtained through blood analysis of samples collected at four different moments (M1-fasting; M2-1.5 hr after caloric intake; M3-Immediately after the simulated fight; M4-24 hr after the simulated fight). Responses triggered by the simulated fight between baseline and after 24 hr were found to increase in monocyte (100%), neutrophil (20%), and insulin (57%) concentrations, while reductions were observed in eosinophils (−50%), lymphocytes (−26.6%), platelets (−22%), cortisol (−50%), and creatine phosphokinase (−45.2%). After 24 hr lactate values returned to baseline levels. The different changes in biochemical and hematological parameters observed constitute responses to acute physical exercise and were according to the level of the high performance athlete. From these data it will be possible to evaluate the periodization, training load, and recovery techniques according to the individual response verified. In addition, these data may be used for comparison purposes within this specific sport, whose literature is still limited.

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Section: F I G U R Esupporting

confidence: 91%

Section: F I G U R Ementioning

confidence: 96%

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Hematological parameters in champion of Brazilian jiu‐jitsu paradesport: Case study

et al. 2020

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“…Another study combined microfiltration and microchip enzyme-linked immunosorbent assay (ELISA) methods to collect EBCCs for BC detection. The device reported a sensitivity of 77.1% and a specificity of 90% 128 . Overall, even though some of these microfluidic-based techniques can achieve high separation efficiency, certain parameters such as the use of external fields may lead to low throughput.…”

Section: Upcoming Technological Advancements To Facilitate Biomarker mentioning

confidence: 96%

Urine biopsy technologies: Cancer and beyond

Chen¹,

Liao²,

Li³

et al. 2020

Theranostics

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Since the discovery of circulating tumor cells in 1869, technological advances in the study of biomarkers from liquid biopsy have made it possible to diagnose disease in a less invasive way. Although blood-based liquid biopsy has been used extensively for the detection of solid tumors and immune diseases, the potential of urine-based liquid biopsy has not been fully explored. Advancements in technologies for the harvesting and analysis of biomarkers are providing new opportunities for the characterization of other disease types. Liquid biopsy markers such as exfoliated bladder cancer cells, cell-free DNA (cfDNA), and exosomes have the potential to change the nature of disease management and care, as they allow a cost-effective and convenient mode of patient monitoring throughout treatment. In this review, we addressed the advancement of research in the field of disease detection for the key liquid biopsy markers such as cancer cells, cfDNA, and exosomes, with an emphasis on urine-based liquid biopsy. First, we highlighted key technologies that were widely available and used extensively for clinical urine sample analysis. Next, we presented recent technological developments in cell and genetic research, with implications for the detection of other types of diseases, besides cancer. We then concluded with some discussions on these areas, emphasizing the role of microfluidics and artificial intelligence in advancing point-of-care applications. We believe that the benefits of urine biopsy provide diagnostic development potential, which will pave opportunities for new ways to guide treatment selections and facilitate precision disease therapies.

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“…[102] Later, the same group reported another integrated microfiltration device to isolate and enrich ETCs from urine samples and revealed an obviously higher number of ETCs from bladder cancer patients (Figure 1D). [103] Another work combining microfiltration with droplet digital polymerase chain reaction (ddPCR) allowed the capture of urinary exfoliated prostate cancer cells and the analysis of DNA methylation biomarkers, offering a novel tool to detect high-grade prostate cancer. [104] In brief, hydrodynamic trapping is also a competent microfluidic technique for the capture, isolation and enrichment of ETCs and EVs for urine biopsy.…”

Section: Passive Hydrodynamic Trappingmentioning

confidence: 99%

Microfluidics‐Based Urine Biopsy for Cancer Diagnosis: Recent Advances and Future Trends

2022

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Urine biopsy, allowing for the detection, analysis and monitoring of numerous cancer-associated urinary biomarkers to provide insights into cancer occurrence, progression and metastasis, has emerged as an attractive liquid biopsy strategy with enormous advantages over traditional tissue biopsy, such as non-invasiveness, large sample volume, and simple sampling operation. Microfluidics enables precise manipulation of fluids in a tiny chip and exhibits outstanding performance in urine biopsy owing to its minimization, low cost, high integration, high throughput and low sample consumption. Herein, we review recent advances in microfluidic techniques employed in urine biopsy for cancer detection. After briefly summarizing the major urinary biomarkers used for cancer diagnosis, we provide an overview of the typical microfluidic techniques utilized to develop urine biopsy devices. Some prospects along with the major challenges to be addressed for the future of microfluidicbased urine biopsy are also discussed.

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Microchips for detection of exfoliated tumor cells in urine for identification of bladder cancer

Cited by 12 publications

References 24 publications

Hematological parameters in champion of Brazilian jiu‐jitsu paradesport: Case study

Hematological parameters in champion of Brazilian jiu‐jitsu paradesport: Case study

Urine biopsy technologies: Cancer and beyond

Microfluidics‐Based Urine Biopsy for Cancer Diagnosis: Recent Advances and Future Trends

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