Microcomputer video image analysis

Jarvis, L. R.

doi:10.1111/j.1365-2818.1988.tb04601.x

Cited by 44 publications

(16 citation statements)

References 33 publications

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“…The dissolution process was filmed by the LSCM and recorded to video, and the temperature and time were logged throughout the experiment. The recording would then be digitally analysed using an image analysis software SCAN IMAGE 14 to obtain inclusion dimensions. The heating profile used on all experiments is shown in Fig.…”

Section: Experimental Experimental Proceduresmentioning

confidence: 99%

Effect of changing slag composition on spinel inclusion dissolution

Monaghan¹,

Chen²

2006

Ironmaking & Steelmaking

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The rate of MgAl 2 O 4 spinel inclusion dissolution in CaO-SiO 2 -Al 2 O 3 slags at 1504uC has been measured using a laser scanning confocal microscope. It was found that the mechanism of spinel inclusion dissolution was at least in part controlled by mass transfer in the slag phase for the slag compositions used. Evidence in support of this finding was that the calculated diffusion coefficient was inversely proportional to the slag viscosity and that the diffusion coefficients were in reasonable agreement with those obtained in a separate study on alumina dissolution. The diffusion coefficients obtained were in the range of 0 . 76-2 . 2610 210 m 2 s 21 .

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Section: Experimental Experimental Proceduresmentioning

confidence: 99%

Effect of changing slag composition on spinel inclusion dissolution

Monaghan¹,

Chen²

2006

Ironmaking & Steelmaking

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“…Video image analysis of ERICA stained aspirates The video image analytical system used in this study was developed in our laboratories (Jarvis, 1986(Jarvis, , 1987(Jarvis, , 1988. In brief, the system comprises a microscope, solid state camera, video digitiser, microcomputer and digitiser tablet (Jarvis, 1988).…”

Section: Tissuesmentioning

confidence: 99%

“…Fine needle aspiration (FNA) biopsy is a proven effective procedure for the diagnosis of malignant diseases of the human breast , and a simultaneous evaluation of FN aspirates for the heterogeneity of oestrogen receptor expression within the cancer cell population would yield an early assessment of prognosis without recourse to surgery. The newly emerging technique of video image analysis promises to remove much of the subjectivity integral to semi-quantitative assessments of immunocytochemical staining (Charpin et al, 1986).Our aims in performing this study were: (1) Video image analysis of ERICA stained aspirates The video image analytical system used in this study was developed in our laboratories (Jarvis, 1986(Jarvis, , 1987(Jarvis, , 1988. In brief, the system comprises a microscope, solid state camera, video digitiser, microcomputer and digitiser tablet (Jarvis, 1988).…”

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confidence: 99%

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Immunocytochemical assay for oestrogen receptor in fine needle aspirates of breast cancer by video image analysis

Horsfall

Jarvis²,

Grimbaldeston³

et al. 1989

Br J Cancer

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Summary Assessment of heterogeneity in oestrogen receptor (ER) expression aims to improve prediction of prognosis and treatment assignment in breast cancer. Current assessments are performed manually and are subjective. Automated image analysis as described here objectively quantitates ER in breast cancer nuclei obtained by needle aspiration. ER was visualised by ERICA with diaminobenzidine (DAB) substrate. Various indices of ER positivity were derived from the integrated density and average density measurements of nuclear DAB. Each index was compensated for background staining by non-specific antibody binding and endogenous peroxidase activity. Total nuclear ER content (iAntegrated optical density of stain) was strongly associated with the biopsy ER concentration determined by shturation analysis of radioligand binding (DCC), P < 0.005. Nuclear ER concentration by image analysis (mean optical density of stain) was The presence of oestrogen and progesterone receptors in breast cancer tissue is now accepted as indicating a better overall disease prognosis for the patient, and as a marker for assisting the selection of patients who may benefit from endocrine therapies (Cant et al., 1985;McGuire, 1987;Thorpe et al., 1986;Williams et al., 1987).However, the power of prediction for good clinical endocrine response which is afforded by hormone receptor quantitation could be improved considerably at the individual patient level. The current feeling is that a knowledge of the heterogeneity of nuclear oestrogen receptor (ER) expression could be helpful in improving the reliability of patient assessment, and thereby assist in the assignment of appropriate treatment. Fine needle aspiration (FNA) biopsy is a proven effective procedure for the diagnosis of malignant diseases of the human breast , and a simultaneous evaluation of FN aspirates for the heterogeneity of oestrogen receptor expression within the cancer cell population would yield an early assessment of prognosis without recourse to surgery. The newly emerging technique of video image analysis promises to remove much of the subjectivity integral to semi-quantitative assessments of immunocytochemical staining (Charpin et al., 1986).Our aims in performing this study were: (1) Video image analysis of ERICA stained aspirates The video image analytical system used in this study was developed in our laboratories (Jarvis, 1986(Jarvis, , 1987(Jarvis, , 1988. In brief, the system comprises a microscope, solid state camera, video digitiser, microcomputer and digitiser tablet (Jarvis, 1988). The image analysis software permits selection and scoring of individual tumour cells through editing of debris, overlapping or touching nuclei, stromal cells or any other undesired feature. The editing functions are performed by a Br.

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“…Video image analysis (VIA) enables measurement on cells in tissue sections. The structure of image analysis system is reviewed in detail elsewhere (Skinner et al, 1993;Jarvis, 1988Jarvis, ,1992 but generally comprises a video camera mounted at the head of a microscope, a microcomputer with digitizer card and relevant software, a video screen, and a pointer (mouse) or digitizer pad. The microscopic image of immunostained cells is digitized and captured on the video screen and a threshold set so that the reaction product is shown highlighted on the screen.…”

Section: Introductionmentioning

confidence: 99%

A comparison of the sensitivity of immunoperoxidase staining methods with high-sensitivity fluorescence flow cytometry-antibody quantitation on the cell surface.

Coventry¹,

Neoh²,

Mantzioris³

et al. 1994

J Histochem Cytochem.

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Surface molecules present in low copy numbers can be detected with high-sensitivity fluorescence flow cytometry. Many cells previously thought not to express certain molecules on their surface can now be shown to have these molecules in very low copy numbers by high-sensitivity fluorescent cytometric methods. Detection of molecules by immunoperoxidase staining methods has not previously been compared with high-sensitivity flow cytometry techniques. Computerized video image analysis (VIA) is a method that allows measurement of area and density of the immunostain chromogen reaction product in a standardized fashion analogous to flow cytometry. In this study, we compared immunoperoxidase reaction products measured by VIA methods with high-sensitivity flow cytometric measurements for cells with 10,000 down to 50 antibody molecules bound to their surfaces. Detection of 100-200 surface molecules was possible with heavy metal-enhanced immunoperoxidase methods, whereas standard immunoperoxidase methods were not as sensitive. The sensitivity of the nickel-enhanced immunoperoxidase staining method was confirmed for detection of an epitope (Tac-IL2 receptor alpha-chain) present in low numbers on the surface of peripheral blood lymphocytes.

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Microcomputer video image analysis

Cited by 44 publications

References 33 publications

Effect of changing slag composition on spinel inclusion dissolution

Effect of changing slag composition on spinel inclusion dissolution

Immunocytochemical assay for oestrogen receptor in fine needle aspirates of breast cancer by video image analysis

A comparison of the sensitivity of immunoperoxidase staining methods with high-sensitivity fluorescence flow cytometry-antibody quantitation on the cell surface.

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