Microcontact imprinting based surface plasmon resonance (SPR) biosensor for real-time and ultrasensitive detection of prostate specific antigen (PSA) from clinical samples

Ertürk, Gizem; Özen, Haluk; Tümer, Murat; Mattìasson, Bo; Deni̇zli̇, Adil

doi:10.1016/j.snb.2015.10.093

Cited by 192 publications

(70 citation statements)

References 54 publications

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“…Taking inspiration from the high sensitivity and selectivity of SPR‐based biosensors, Erturk et al developed a microcontact PSA‐imprinted SPR biosensor chip for sensitive, real‐time detection of PSA . The molecular imprinting of methacrylic acid (MAA) (functional monomer) on a gold coated SPR chip was achieved in the presence of ethylene glycol dimethacrylate (EGDMA) (crosslinking agent) by a UV induced photo polymerization process.…”

Section: Nanomaterial‐based Cancer Sensing Systemsmentioning

confidence: 99%

“…The molecular imprinting of methacrylic acid (MAA) (functional monomer) on a gold coated SPR chip was achieved in the presence of ethylene glycol dimethacrylate (EGDMA) (crosslinking agent) by a UV induced photo polymerization process. During tests with standard PSA solutions, detection was achieved within a concentration range of 0.1–50 ng mL −1 with a recorded DL of 91 pg mL −1 (18 × 10 −14 m ) . The prepared SPR‐based biosensor also showed high selectivity in the presence of other competitive agents, such as human serum albumin (HSA) and lysozyme (Lyz).…”

Section: Nanomaterial‐based Cancer Sensing Systemsmentioning

confidence: 99%

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Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Kumar

Kukkar

Hashemi

et al. 2019

Adv Funct Materials

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Cancer is the second most common cause of death in the world. The principal limitations thus far encountered in the clinical practice of probing cancer are diverse and include low sensitivity, time consumption, bulkiness, and cost. In this respect, nanomaterial (NM)-based sensing techniques are recognized as a superior alternative to efficiently resolve such limitations. A better understanding of NM-based sensing platforms is thus important so that these novel avenues can easily be explored for clinical applications. These platforms have the merits of high sensitivity, high specificity, rapid response, and easy-to-read signals. This review offers a comprehensive survey of NM-based advanced cancer-sensing techniques and will help the scientific community establish optimum sensing strategies based on an accurate assessment of the interactions between cancer biomarkers and NM-based platforms.but are not limited to, carcinoma, sarcoma, leukemia, lymphoma, non-Hodgkin lymphoma, myeloma, central nervous system cancer, lung and bronchus cancer, colon cancer, rectum cancer, prostate cancer, bladder cancer, kidney and renal pelvis cancer, endometrial cancer, pancreatic cancer, liver cancer, and thyroid cancers. [1] Moreover, the most commonly occurring cancers tend to differ by common criteria like sex and age. For example, men are at risk for prostate, lung, and colorectal cancer, women are at risk for breast, lung and colorectal cancer, and children are at risk for leukemia, brain tumors, and lymphoma. The severity of the cancer depends upon the development level or staging of the disease. [2] Likewise, the type of treatment required to treat the cancer is determined by the staging of the disease.The more severe condition of cancer is metastasis, i.e., the uncontrolled growth of cells that invade surrounding tissues. The metastasis condition of cancer can affect any organ of the body. [3] As per the WHO 2010 report on cancer, the lives of 30% patients could have been saved if the cancer was diagnosed earlier. The detection of cancer is not easy because the immune system does not necessarily consider cancerous cells as foreign bodies. However, a few changes (such as altered biomarker levels and the presence of cancerous cells in biological fluids) can be detected. [4] In general, cancer biomarkers are overexpressed proteins present in blood/serum and at the cancer cell surface. The accurate and efficient detection of these biomarkers can be useful for cancer detection. The major problem with biomarkers is their low level in the body during the earlier stages of cancer. Hence, an efficient sensor/device capable of detecting these molecules even at very low levels, if developed, could help the clinician efficiently diagnose cancer cells in the human body.The sensing techniques presently used by clinicians suffer from several limitations; specifically, they are time consuming, bulky, have low sensitivity, and are expensive. Nanomaterial (NM)-based techniques have become an important alternative to the laborious conventional...

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Section: Nanomaterial‐based Cancer Sensing Systemsmentioning

confidence: 99%

Section: Nanomaterial‐based Cancer Sensing Systemsmentioning

confidence: 99%

Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Kumar

Kukkar

Hashemi

et al. 2019

Adv Funct Materials

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“…To handle this issue and further enhance detection sensitivity, gold nanoparticles (AuNPs), serving as amplification labels, have been widely introduced into the metallic nanoparticle-LSPR-based biochip for analytical signal amplification to improve detection performance owing to its further plasmonic coupling, strong biocompatibility, and ease of modification. [6][7][8][9] For example, Duyne and coworkers reported an LSPR bioassay for the detection of surfacebound analytes through the integration of gold nanoparticleconjugated antibodies and the biotin and antibiotin binding system. [10] The observed shift of binding of the gold nanoparticle-labeled antibiotins was achieved up to a 400% amplification compared to the unlabeled antibiotin system.…”

Section: Introductionmentioning

confidence: 99%

Immunoassay of plasmonic gold‐nanoparticle clusters: Plasmon coupling effects for Parkinson biomarker detection

Chang

Chiang

et al. 2019

J Chinese Chemical Soc

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A simple signal‐on plasmonic optical assay for the detection of the Parkinson biomarker using gold‐nanoparticle clusters (AuNCs) for signal amplification is presented. This approach is based on the improvement of the optical density (OD) change of the plasmonic band of a localized surface plasmon resonance (LSPR) Au nanoparticle (AuNP) sensor interface using Au NCs conjugated antibodies. The amplification results in a 260‐fold improvement in concentration detection, from 1,000 ng/mL (unlabeled antibody) to 3.8 ng/mL (antibody‐conjugated AuNCs). The sensitivity enhancement can be ascribed to the further plasmonic coupling between the antibody‐conjugated AuNCs and the AuNPs on the LSPR interface and the enhanced amount of target molecule bound to the bioassay. This AuNCs‐assisted signal amplification strategy allows for improving the sensitivity of the plasmon‐based bioassays and can be extended to other optical‐based diagnostic technologies. Importantly, the simple detecting procedure and protocol assembly make it competitive with other existing sensing technologies such as ELISA, allowing for practical usage in clinical diagnostics.

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“…MIPs have been produced to capture a broad range of compounds, from low‐molecular‐weight structures to metal ions (Aslıyüce et al, ), pharmaceuticals, environmental pollutants (Üzek, Sari, Şenel, Denizli, & Merkoçi, ), proteins (Perçin, Idil, & Denizli, ), virus particles (Gast, Sobek, & Mizaikoff, ) and even whole microbial cells (Idil, Hedström, Denizli, & Mattiasson, ). There are applications in both bioseparation and diagnostics (Bereli et al, ; Bereli, Saylan, Uzun, Say, & Denizli, ; Ertürk, Özen, Tümer, Mattiasson, & Denizli, ; Uzun, Say, Ünal, & Denizli, ). The target molecule in this study was protein A, which is a surface protein on the cell wall of Staphylococcus aureus .…”

Section: Introductionmentioning

confidence: 99%

Combined protein A imprinting and cryogelation for production of spherical affinity material

Aslıyüce

Mattìasson

Deni̇zli̇

2019

Biomedical Chromatography

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Cryogels have been demonstrated to be efficient when applied for protein isolation.Owing to their macroporous structure, cryogels can also be used for treating particle-containing material, e.g. cell homogenates. Another challenging development in protein purification technology is the use of molecularly imprinted polymers (MIPs).These MIPs are robust and can be used repeatedly. The paper presents a new technology that combine the formation of cryogel beads concomitantly with making imprints of a protein. Protein A was chosen as the print molecule which was also be the target in the purification step. The present paper describes a new method to produce protein-imprinted cryogel beads. The protein-imprinted material was characterized and the separation properties were evaluated with regard to both the target protein and whole cells with target protein exposed on the cell surface. The maximum protein A adsorption was 18.1 mg/g of wet cryogel beads. The selectivity coefficient of protein A-imprinted cryogel beads for protein A was 5.44 and 12.56 times greater than for the Fc fragment of IgG and protein G, respectively.

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Microcontact imprinting based surface plasmon resonance (SPR) biosensor for real-time and ultrasensitive detection of prostate specific antigen (PSA) from clinical samples

Cited by 192 publications

References 54 publications

Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Advanced Functional Structure‐Based Sensing and Imaging Strategies for Cancer Detection: Possibilities, Opportunities, Challenges, and Prospects

Immunoassay of plasmonic gold‐nanoparticle clusters: Plasmon coupling effects for Parkinson biomarker detection

Combined protein A imprinting and cryogelation for production of spherical affinity material

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