Diabetes is one of the most chronic diseases, with an increasing number of sufferers yearly. It can lead to several serious complications, including diabetic peripheral neuropathy (DPN). DPN must be recognized early to receive appropriate treatment and prevent disease exacerbation. However, due to the rapid development of machine learning classification, like in the health science sector, it is very easy to identify DPN in the early stages. Therefore, the aim of this study is to develop a new method for detecting neuropathy based on the myoelectric signal among diabetes patients at a low cost with utilizing one of the machine learning techniques, the artificial neural network (ANN). To that aim, muscle sensor V3 is used to record the activity of the anterior tibialis muscle. Then, the representative time domain features which is mean absolute value (MAV), root mean square (RMS), variance (VAR), and standard deviation (SD) used to evaluate fatigue. During neural network training, a different number of hidden neurons were used, and it was found that using seven hidden neurons showed a high accuracy of 98.6%. Thus, this work indicates the potential of a low-cost system for classifying healthy and diabetic individuals using an ANN algorithm.