Microcosm Biofilm Formation on Titanium Surfaces

Dal’Agnol, Claudio Zeferino; Stefenon, Letícia; Sande, Françoise Hélène van de; Bona, Álvaro Della; Cenci, Maximiliano Sérgio; Webber, Bruna; Rodrigues, Laura Beatriz; Santos, Luciana Ruschel dos

doi:10.1590/1516-1439.346814

Cited by 3 publications

(4 citation statements)

References 47 publications

(53 reference statements)

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“…There is controversy on when is the critical point in biofilm development in which surface roughness could have a bigger impact (adhesion, growth or maturation). Some studies have reported a greater influence at early stages(Al‐Ahmad et al, ; Dal Agnol et al, ; Frojd et al, ; Lin, Liu, Wismeijer, Crielaard, & Deng, ), while others have shown a higher impact of rougher surfaces with increasing amounts of bacteria, as biofilms become mature(Al‐Ahmad et al, ; John, Becker, & Schwarz, ). Further studies are needed to identify the critical point in biofilm development for surface topography to have a bigger impact.…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

Bermejo

Sanchez

Llama‐Palacios

et al. 2019

Clinical Oral Implants Res

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Objectives:To compare biofilm formation on whole dental titanium implants with different surface micro-topography. Methods:A multispecies in vitro biofilm model consisting of initial (Streptococcus oralis and Actinomyces naeslundii), early (Veillonella parvula), secondary (Fusobacterium nucleatum) and late colonizers (Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans) was grown for 96 hr on sterile titanium dental implants with either minimal (S a : 0.5-1.0 mm) or moderate-roughness titanium surfaces (S a : 1.1-2.0 mm).The resulting biofilms were studied with Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscope. Concentrations (colony-forming units per mL [CFU/ml]) of each bacterium were measured by quantitative Polymerase Chain Reaction (qPCR) and compared by Student t tests.Results: A biofilm, located mainly at the peak and lateral areas of the implant threads, was observed on both implant surfaces, with a greater biomass and a greater live/dead ratio in moderate-compared to minimal-roughness surface implants. Statistically significant higher values of total bacteria (mean difference = 2.61 × 10 7 CFU/ml; 95% confidence interval -CI [1.91 × 10 6 ; 5.02 × 10 7 ]; p = 0.036), F. Nucleatum (mean difference = 4.43 × 10 6 CFU/ml; 95% CI [1.06 × 10 6 ; 7.80 × 10 6 ]; p = 0.013) andA. actinomycetemcomitans (mean difference = 2.55 × 10 7 CFU/ml; 95% CI [1.07 × 10 7 ; 4.04 × 10 7 ]; p = 0.002), were found in the moderate-compared to minimal-roughness surface dental implants.Conclusions: Implants with moderate-roughness surfaces accumulated more bacterial biomass and significant higher number of pathogenic bacteria (F. nucleatum and A. actinomycetemcomitans), when compared to implants with minimal-roughness surfaces, within a similar biofilm structure.

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Section: Discussionmentioning

confidence: 99%

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

Bermejo

Sanchez

Llama‐Palacios

et al. 2019

Clinical Oral Implants Res

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“…It was followed by the release of the commercially labeled MTA Angelus ® (Angelus Soluções Odontológicas, Londrina, Brazil). Nevertheless, potential drawbacks such as extended setting time, tooth discoloration, elevated cost, and challenging handling characteristics have emerged [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Properties, Cell Viability, and Metalloproteinase Activity of Bioceramic Endodontic Materials Used in Vital Pulp Therapy

Immich,

de Oliveira,

Ribeiro de Andrade

et al. 2024

JFB

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This study aimed to evaluate the antimicrobial properties, cell viability, and matrix metalloproteinase (MMP) inhibition capacity of several endodontic materials aimed at vital pulp therapy: Pro Root MTA®, EndoSequence®, Biodentine®, MTA Angelus®, TheraCal LC®, and BioC Repair®. The materials were prepared according to the manufacturer’s instructions. Antimicrobial tests were conducted using a microcosm biofilm model, cell viability was assessed using murine fibroblasts (L929), and MMP activity was analyzed through electrophoresis. The results showed that BioC Repair®, Biodentine®, and EndoSequence® exhibited similar antimicrobial properties, while MTA Angelus® and ProRoot MTA® had inferior results but were comparable to each other. In terms of cell viability, no significant differences were observed among the materials. EndoSequence® demonstrated the highest MMP inhibition capacity. In conclusion, BioC Repair®, Biodentine®, EndoSequence®, and TheraCal® showed better antimicrobial properties among the tested materials. The materials did not exhibit significant differences in terms of cytotoxicity. However, EndoSequence® displayed superior MMP inhibition capacity.

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“…A moderately rough surface, as developed here [41], is also considered to favour osseointegration due to increased attachment of the blood clot and, later, of osteoblast precursor cells and thus bone ingrowth [1,33,40,42,43]. Whilst many microscopically rough surfaces allow formation of undesirable bacterial biofilms [11,12], an R a of 1-2 µm is considered to give the best compromise with regards to facilitating adhesion of hMSCs and ultimately osseointegration [41]. As such, the physical surface characteristics for the SLA Ti surfaces developed here could be expected to facilitate osseointegration.…”

Section: Discussionmentioning

confidence: 99%

“…There is a high chance of peri-operative bacterial contamination from various sources [10]. Bacteria readily adhere to roughened implant surfaces [11,12] and can form biofilms, which disrupt osseointegration of the implant by mesenchymal stem cells (MSCs). This has been hypothesised as one cause of early implant failures [8].…”

Section: Introductionmentioning

confidence: 99%

A novel chlorhexidine-hexametaphosphate coating for titanium with antibiofilm efficacy and stem cell cytocompatibility

Garner

Dalby

Nobbs

et al. 2021

J Mater Sci: Mater Med

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Dental implants are an increasingly popular way to replace missing teeth. Whilst implant survival rates are high, a small number fail soon after placement, with various factors, including bacterial contamination, capable of disrupting osseointegration. This work describes the development of chlorhexidine-hexametaphosphate coatings for titanium that hydrolyse to release the antiseptic agent chlorhexidine. The aim was to develop a coating for titanium that released sufficient chlorhexidine to prevent biofilm formation, whilst simultaneously maintaining cytocompatibility with cells involved in osseointegration. The coatings were characterised with respect to physical properties, after which antibiofilm efficacy was investigated using a multispecies biofilm model, and cytocompatibility determined using human mesenchymal stem cells. The coatings exhibited similar physicochemical properties to some implant surfaces in clinical use, and significantly reduced formation of multispecies biofilm biomass up to 72 h. One coating had superior cytocompatibility, with mesenchymal stem cells able to perform normal functions and commence osteoblastic differentiation, although at a slower rate than those grown on uncoated titanium. With further refinement, these coatings may have application in the prevention of bacterial contamination of dental implants at the time of surgery. This could aid a reduction in rates of early implant failure.

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Microcosm Biofilm Formation on Titanium Surfaces

Cited by 3 publications

References 47 publications

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

Biofilm formation on dental implants with different surface micro‐topography: An in vitro study

Evaluation of Antimicrobial Properties, Cell Viability, and Metalloproteinase Activity of Bioceramic Endodontic Materials Used in Vital Pulp Therapy

A novel chlorhexidine-hexametaphosphate coating for titanium with antibiofilm efficacy and stem cell cytocompatibility

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