Microcrystallization of α-lactalbumin

Lee, Mi Ji; Kwon, Jai Hyun; Shin, Juyeon; Kim, Chan Wha

doi:10.1016/j.jcrysgro.2005.05.012

Cited by 10 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…33 The DSC thermogram of ALA did not exhibit a melting peak, implying its amorphous nature (Figure 1b). 34 On the contrary, PCL displayed a well-defined endothermic signal corresponding to its melting point at 61.8 °C, signifying its crystalline and anhydrous characteristics. 35,36 The thermal peak of PCL in the physical mixtures was attenuated.…”

Section: Resultsmentioning

confidence: 98%

α-Lactalbumin-Based Nanofiber Dressings Improve Burn Wound Healing and Reduce Scarring

Guo

Liu

Bera

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Skin wound especially burn injury is a major threat for public health. One of the pursuits in the current wound healing research is to identify new promising biological materials, which can not only promote tissue repair but also reduce scar formation. In this current study, the potentials of α-lactalbumin (ALA), a tryptophan-rich dietary protein acting as a precursor of neurotransmitter serotonin, to promote the burn wound healing and reduce the scar formation were investigated. The ALA was initially electrospun with polycaprolactone (PCL) to accomplish electrospun nanofibrous mats (ENMs), subsequently assessed for their physicochemical attributes and wound healing efficiency on a burn rat model, and then their healing mechanisms at cellular and molecular levels were explored. The results showed that ALA and PCL were physicochemically compatible in ENMs. The average diameter of various nanofibers was within 183−344 nm. Their wettability and mechanical properties could be readily modulated by adjusting the mass ratios of ALA and PCL from 1/9 to 1/2. The selected ENMs exhibited negligible cytotoxicity and satisfactory adhesion to fibroblasts and promoting the proliferation of the fibroblasts. As compared to pristine PCL based ENMs, the composite scaffolds could accelerate the wound healing process and exhibit effects comparable to a marketed wound dressing over 16 days. Moreover, the ALA/PCL based ENMs could increase the synthesis of type I collagen and decrease the expression of α-smooth muscle actin, conferring that the novel wound dressings could reduce the formation of scars. Collectively, this study demonstrates that the ALA is a promising biological material and could promote the regeneration of burn skins with reduced scar formation, when being loaded on ultrafine fibrous scaffolds, mimicking the structure of the natural extra cellular matrix.

show abstract

Section: Resultsmentioning

confidence: 98%

α-Lactalbumin-Based Nanofiber Dressings Improve Burn Wound Healing and Reduce Scarring

Guo

Liu

Bera

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…55 Amorphous protein particles are cleared rapidly from systemic circulation and are more susceptible to hydrolytic and enzymatic degradation because of their higher reactivity. 56 These factors make crystalline protein desirable as a fine pharmaceutical ingredient. Crystallization of proteins results in high-purity product.…”

Section: Crystallization As a Tool For Preparation Of Inhalable Drug ...mentioning

confidence: 99%

Particle Engineering Strategies via Crystallization for Pulmonary Drug Delivery

Ragab

Rohani

2009

Org. Process Res. Dev.

View full text Add to dashboard Cite

This review covers recent developments in the area of particle engineering via crystallization for pulmonary drug delivery. The past decade has witnessed a shift from empirical formulation efforts to an engineering approach based on a better understanding of the crystallization process. Microcrystals with nanoscale substructures can now be designed and their functionality has contributed significantly to the stability and efficacy of the particulate dosage form. This review provides concepts and a theoretical framework for particle design calculations. It reviews experimental research to identify variables that influence particle formation. It offers an explanation of how excipient properties in combination with process variables influence the morphology of the engineered particles. A wide range of pharmaceutical applications of large porous particles, particles with low surface energy, and particle aggregates, is discussed, with specific emphasis on the underlying crystal formation mechanism and design concepts.

show abstract

“…The basics of protein crystallization conform to the classical understanding of small molecules crystallisation, with the main difference from small molecule crystal growth being the consequent greater sensitivity of proteins to external conditions (Ducruix and Giegé 1999). Protein structure determination has long been considered to be an important tool, by allowing for example the design of novel drugs on the basis of the structures of protein therapeutic targets (Lee et al 2005), and therefore plays a key role in structure based drug discovery and structural genomics projects (Chayen 2004). Crystallization of macromolecules remains a problematic and complex process owing to the large size and the frequent flexibility of these molecules often composed of several subunits.…”

Section: Introductionmentioning

confidence: 99%

Crystallization, solubility and thermodynamics of the highly thermostable glucose isomerase from Streptomyces sp. strain

Borgi¹,

Rhimi²,

Kadri³

2014

gpb

View full text Add to dashboard Cite

Abstract. The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility increased with increasing temperature and decreased with increasing salt concentration. The temperature-dependent solubility was used to characterize the thermodynamic parameters of crystallization such as enthalpy, entropy and free energy.

show abstract

Microcrystallization of α-lactalbumin

Cited by 10 publications

References 19 publications

α-Lactalbumin-Based Nanofiber Dressings Improve Burn Wound Healing and Reduce Scarring

α-Lactalbumin-Based Nanofiber Dressings Improve Burn Wound Healing and Reduce Scarring

Particle Engineering Strategies via Crystallization for Pulmonary Drug Delivery

Crystallization, solubility and thermodynamics of the highly thermostable glucose isomerase from Streptomyces sp. strain

Contact Info

Product

Resources

About