MicroCT Imaging of Heart Valve Tissue in Fluid

Stephens, Sam; Bean, M.; Surber, H.; Ingels, Neil B.; Jensen, Henrik; Liachenko, Serguei; Wenk, Jonathan F.; Jensen, Morten Ø.

doi:10.1007/s11340-020-00667-5

Cited by 2 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effect of venous tissue rolling on itself during mounting, and when submerged in the water bath for the fresh samples, is similar to that observed for cardiac valve tissue in air. 21,22 This observation is important to note in the experimental context, but it is not indicative of how the xenograft in a bioprosthesis would behave in vivo as the viscosity of blood is 4x higher than that of distilled water. The significantly higher shrink temperature of both vein wall and venous valve leaflets for glutaraldehyde compared to the fresh tissue demonstrates the crosslinking of collagen and thermal stability that occurs with fixation.…”

Section: Pronase Digestion (% Remaining)mentioning

confidence: 99%

Glutaraldehyde fixation of venous valve tissue: A benchmark for alternative fixation methods

et al. 2022

View full text Add to dashboard Cite

Objective Bioprosthetic venous valves have yet to achieve long-term patency due to issues with calcification following implantation that is influenced by current xenograft fixation methods, most notably glutaraldehyde. The goal of this study was to investigate the effects of glutaraldehyde fixation on the functional properties of venous tissue to establish a benchmark for the evaluation of alternative fixation methods. Methods The degree of crosslinking was evaluated by determining shrink temperature and the stability of tissue with pronase and collagenase digestion. Results Glutaraldehyde fixation of venous tissue was confirmed by a significant difference in the shrink temperature between fresh and glutaraldehyde treated samples. Significant differences in the amount of tissue remaining following digestion were observed for venous versus cardiac tissue. Conclusions This study demonstrates the importance of tissue-specific evaluation in the development of alternative xenograft fixation methods to improve outcomes with bioprosthetic venous valves.

show abstract

Section: Pronase Digestion (% Remaining)mentioning

confidence: 99%