Microcystin-LR induced cellular effects in mammalian and fish primary hepatocyte cultures and cell lines: A comparative study

Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)

“…Mitochondria are known to be vulnerable targets of various toxins because of their important role in maintaining cellular structures and functions (Robertson et al, 2004). It is reported that MC-LR could induce morphological changes of mitochondria, uncoupling of mitochondrial electron transport and production of ROS, leading to cell apoptosis in in vitro cultured hepatocytes (Li et al, 2003;Ding and Ong, 2003;Boaru et al, 2006) and in vivo liver (Li et al, 2004;Qiu et al, 2007). Furthermore, in our study, the severity of the injury in renal tubules was much higher than that in glomeruli, suggests that renal tubules are the more sensitive, vulnerable parts of the kidney tissues in fish exposed to MCs.…”

In vivo studies on the toxic effects of microcystins on mitochondrial electron transport chain and ion regulation in liver and heart of rabbit

“…It is reported that MC-LR could induce morphological changes of mitochondria, uncoupling of mitochondrial electron transport and production of ROS, leading to cell apoptosis in in vitro cultured mammalian and fish cells (Ding and Ong, 2003;Boaru et al, 2006;Xing et al, in press). In the present in vivo study, MC exposure induced two typical ultrastructural changes of mitochondria: concentration of cristae and matrix with obvious vacuolization in the low dose group and loss of their metrical density with highly swollen forms in the high dose group.…”

In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii

“…The lower sensitivity of the fish cells toward microcystins (compared to the mammalian cells) in our study could be explained with the absence of detectable mRNA levels of organic anion transporter polypeptides [43]. Boaru et al [43] showed also that microcystin-LR causes damage of the subcellular structures.…”

“…RTgill-W1 cells were used to evaluate the toxicity of industrial waste water, polycyclic aromatic hydrocarbons and heavy metals [37][38][39][40][41][42]. The lower sensitivity of the fish cells toward microcystins (compared to the mammalian cells) in our study could be explained with the absence of detectable mRNA levels of organic anion transporter polypeptides [43]. Boaru et al [43] showed also that microcystin-LR causes damage of the subcellular structures.…”