Microcystin-LR induces oxidative DNA damage in human hepatoma cell line HepG2

Žegura, Bojana; Sedmak, Bojan; Filipič, Metka

doi:10.1016/s0041-0101(02)00207-6

Cited by 208 publications

(123 citation statements)

References 39 publications

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“…One obvious toxic effects of MC-LR is the oxidative stress (Guzman and Solter, 1999;Ding and Ong, 2003;Zegura et al, 2003), and HSPs induction has been suggested as early marker of oxidative stress (Peng et al, 2000). Oxidative stress may occur either due to the over production of reactive oxygen species (ROS) or to decrease of cellular antioxidant levels.…”

Section: Discussionmentioning

confidence: 99%

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Sun

et al. 2009

Aquaculture

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Section: Discussionmentioning

confidence: 99%

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Sun

et al. 2009

Aquaculture

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“…Some studies also attribute to MC's the ability of generate oxidative stress (Jos et al, 2005), increasing the generation of reactive oxygen species (ROS) (Li et al, 2003;Weng et al, 2007) and oxidative damage, such as lipid peroxidation (LPO) (Pinho et al, 2005) and damage to the DNA molecule (Zegura et al, 2003). The ROS are counteracted by the antioxidant defense system, which in aquatic organisms is represented mainly by antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), and non-enzymatic antioxidants as reduced glutathione (GSH) (Livingstone, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…to cyanobacterial cells containing the MC, and the liver was the organ most affected, followed by kidney and gill. Zegura et al (2003) proved, through the exposure of human HepG2 hepatoma cells of MC-LR, that this cyanotoxin is capable of promoting oxidative DNA damage. However, results concerning the effects of MC, more specifically the type LR (MC-LR), in freshwater fishes from the Neotropical region are still scarce.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Garcia

Martinez

2012

Neotrop. ichthyol.

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Microcystins are secondary metabolites produced by different species of cyanobacteria, such as Microcystis aeruginosa (MA). In this study, the biochemical and genetic effects of lyophilized MA were evaluated in the neotropical fish Prochilodus lineatus exposed to 1 or 2 mg L -1 lyophilized MA (treated group) or only water (control group) in static toxicity tests for 24 and 96 h. The gills and liver were used in the analysis of biotransformation enzymes and antioxidant defenses, blood and gill cells in genetic analysis and in brain and muscle it was determined the activity of acetylcholinesterase (AChE). The results showed the biotransformation pathway activation due to the increase in hepatic CYP1A and in branchial and hepatic glutathione Stransferase (GST). The antioxidant defense proved to be greatly affected by MA exposure leading to changes, both in gills and liver, in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and in the content of tripeptide glutathione (GSH). Lipid peroxidation was not detected, but damage to DNA molecule was observed in blood cells. In conclusion, it can be state the lyophilized MA is able to promote changes in the biochemical and genetic parameters of P. lineatus.As microcistinas são metabólitos secundários produzidos por diferentes espécies de cianobactérias, como a Microcystis aeruginosa (MA). Neste estudo, os efeitos bioquímicos e genéticos de liofilizado de MA foram avaliados para juvenis da espécie de peixe Neotropical Prochilodus lineatus expostos a 1 ou 2 mg L -1 de liofilizado de MA (grupo tratado) ou apenas à água (grupo controle), em testes de toxicidade estáticos, durante 24 e 96 h. As brânquias e o fígado foram usados para as análises das enzimas de biotransformação e defesas antioxidantes, células do sangue e das brânquias para análises genéticas e no cérebro e músculo foi determinada a atividade da acetilcolinesterase (AChE). Os resultados mostraram ativação da via de biotransformação devido ao aumento da atividade da CYP1A hepática e da atividade da glutationa S-transferase (GST) hepática e branquial. As defesas antioxidantes foram muito afetadas pela exposição a MA levando a alterações, tanto no fígado como nas brânquias, na atividade da superóxido dismutase (SOD), da catalase (CAT), da glutationa peroxidase (GPx), glutationa redutase (GR) e no conteúdo do tripeptídeo glutationa (GSH). Apesar dessas alterações a peroxidação lipídica não foi detectada em nenhum dos tecidos, mas danos na molécula de DNA foram observados nas células do sangue. Em conclusão, pode-se afirmar que o liofilizado de MA é capaz de promover alterações em parâmetros bioquímicos e genéticos de P. lineatus.

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“…M. aeruginosa and some other freshwater cyanobacteria often produce hepatotoxic cyclic heptapeptides microcystins (Kaya, 1996). The ingestion of intact M. aeruginosa cells or microcystins is reported to be toxic to aquatic organisms, domestic animals, wildlife and humans (Miura et al, 1989;Žegura et al, 2003). Fish mortality was reported to be associated with toxic cyanobacterial water blooms (Andersen et al, 1993;Rodger et al, 1994;Tencalla et al, 1994;Zimba et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary cyanobacteria on growth and accumulation of microcystins in Nile tilapia (Oreochromis niloticus)

et al. 2006

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Microcystin-LR induces oxidative DNA damage in human hepatoma cell line HepG2

Cited by 208 publications

References 39 publications

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Biochemical and genetic alterations in the freshwater neotropical fish Prochilodus lineatus after acute exposure to Microcystis aeruginosa

Effect of dietary cyanobacteria on growth and accumulation of microcystins in Nile tilapia (Oreochromis niloticus)

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