Microcystis botrys and Lemmermanniella flexa - New Species of Cyanoprokaryota for the Flora of Ukraine in Phytoplankton of the Kanev Reservoir

Yarmoshenko, L. P.; Kureyshevich, A. V.; Yakushin, V. M.

doi:10.1615/hydrobj.v49.i2.40

Cited by 4 publications

(3 citation statements)

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“…Group 2 was from Pseudanabaena , another genus of Cyanobacteria . As autotrophic organism, Pseudanabaena mucicola can often be found associated to Microcystis colonies [36,37,38]. The co-cultivation of Pseudanabaena with Microcystis strains in the lab revealed strong interaction between these two cyanobacteria, and evidenced that the growth of Pseudanabaena in natural systems may cause either Microcystis lysis, or colony sedimentation [39].…”

Section: Resultsmentioning

confidence: 99%

Obtaining Genome Sequences of Mutualistic Bacteria in Single Microcystis Colonies

Chen

Gao

et al. 2019

IJMS

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Cells of Microcystis are associated with heterotrophic bacteria and organized in colonies in natural environment, which are basic elements in the mass occurrence of cyanobacterial species. Analyzing these colonies by using metagenomics is helpful to understand species composition and relationship. Meanwhile, the difference in population abundance among Microcystis colonies could be used to recover genome bins from metagenome assemblies. Herein, we designed a pipeline to obtain high-quality genomes of mutualistic bacteria from single natural Microcystis colonies. Single colonies were lysed, and then amplified by using multiple displacement amplification to overcome the DNA quantity limit. A two-step assembly was performed after sequencing and scaffolds were grouped into putative bins based on their differential-coverage among species. We analyzed six natural colonies of three prevailing Microcystis species from Lake Taihu. Clustering results proved that colonies of the same species were similar in the microbial community composition. Eight putative population genome bins with wide bacterial diversity and different GC content were identified based on coverage difference among colonies. At the phylum level, proteobacteria was the most abundant besides cyanobacteria. Six of the population bins were further refined into nearly complete genomes (completeness > 90%).

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Section: Resultsmentioning

confidence: 99%

Obtaining Genome Sequences of Mutualistic Bacteria in Single Microcystis Colonies

Chen

Gao

et al. 2019

IJMS

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“…It is noteworthy that, in some subtropical and temperate lakes, Pseudanabaena has been discovered to co-exist with Microcystis, occasionally even being found in joint succession or dominance patterns [18][19][20]. Studies have shown that the Pseudanabaena biomass was significantly correlated with the Microcystis biomass [19,21], which may be related to an epiphytic relationship between Pseudanabaena and colonial Microcystis [22,23]. During bloom periods, Pseudanabaena mucicola has been identified as epiphytic to colonial Microcystis, leading to the formation of a Microcystis-Pseudanabaena phycosphere [18,24,25].…”

Section: Introductionmentioning

confidence: 99%

Temperature-Dependent Growth Characteristics and Competition of Pseudanabaena and Microcystis

Wang

Cui

et al. 2023

Water

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Global warming has been considered to accelerate the expansion of cyanobacterial blooms, which are frequently composed of the bloom-forming genera, Microcystis and Pseudanabaena, in freshwater ecosystems worldwide. Nonetheless, the impact of changes due to toxin production or lack thereof on the growth of co-existing strains, both arising from increasing temperature, has remained unknown to date. We conducted competition experiments involving toxic Microcystis PCC7806, a non-toxic mcyB− mutant, and two strains of Pseudanabaena (TH-1 and DC-1) identified as P. mucicola. In mono-culture, the specific growth ratio of Pseudanabaena increased; nevertheless, the maximum cell density declined with increasing temperature. The maximum growth ratios of Pseudanabaena TH-1 and Pseudanabaena DC-1 were 0.512 day−1 in the 30 °C group and 0.440 day−1 in the 35 °C group, respectively. The optimum temperature for the growth of Pseudanabaena was 25 °C. Remarkably, there was no significant disparity in the maximum cell density of Microcystis PCC7806 and the mcyB− mutant across varied temperature groups, even though their maximum growth rates differed marginally, reaching 0.280 day−1 and 0.306 day−1 in the 30 °C group, respectively. In co-cultures, the growth of Pseudanabaena TH-1 was uniformly inhibited, whereas that of DC-1 was somewhat influenced by co-culturing with toxic and non-toxic Microcystis, except for the 35 °C group, where inhibition was absent amongst DC-1 and Microcystis. Moreover, the growth of Microcystis was promoted with a co-culture of TH-1 in the 20 °C groups. Conversely, the co-culture of Microcystis with Pseudanabaena DC-1 led to the inhibition of the former in the 30 °C and 35 °C groups. With a Lotka–Volterra competition model, the results showed that Microcystis dominated or co-existed with Pseudanabaena, conforming to expectations from the wild.

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“…Therefore, when compared with Microcystis, the high plasticity of antenna pigments, along with the excellent photoadaptation and photoprotection ability of Pseudanabaena, probably ensures its ecological success under iron limitation when light is sufficient. thrin; rETR max , the maximal relative electron transport rate; TPP/Chl a, total phycobilin pigments to Chl a ratio; a, photosynthetic efficiency; l, the specific growth rate Pseudanabaena is a small, filamentous, and epiphytic cyanobacterial species commonly embedded within or attached to the sheath of colonies of Microcystis, which is a bloom-forming species (Chang 1985, Pankow 1986, Patterson and Wilson 1995, Mayer et al 1997, Vasconcelos and Pereira 2001, Yarmoshenko et al 2013, Zhuang et al 2015, Agha et al 2016. The abundance of these two species is highly correlated during blooms (Ilhe 2008, Berry et al 2017, but most studies focus on Microcystis because of its high abundance and toxin production (de Figueiredo et al 2004).…”

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confidence: 99%

Photosynthetic adaptation to light availability shapes the ecological success of bloom‐forming cyanobacterium Pseudanabaena to iron limitation

Dai

Zhang

et al. 2020

Journal of Phycology

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The poorly understood filamentous cyanobacterium Pseudanabaena is commonly epiphytic on Microcystis colonies and their abundances are often highly correlated during blooms. The response and adaptation of Microcystis to iron limitation have been extensively studied, but the strategies Pseudanabaena uses to respond to iron limitation are largely unknown. Here, physiological responses to iron limitation were compared between one Pseudanabaena and two Microcystis strains grown under different light intensities. The results showed that low‐intensity light exacerbated, but high‐intensity light alleviated, the negative effect of iron limitation on Pseudanabaena growth relative to two Microcystis strains. It was found that robust light‐harvesting and photosynthetic efficiency allowed adaptation of Pseudanabaena to low light availability relative to two Microcystis strains only during iron sufficiency. The results also indicated that a larger investment in the photosynthetic antenna probably contributed to light/iron co‐limitation of Pseudanabaena relative to two Microcystis strains under both light and iron limitation. Furthermore, the lower antenna pigments/chlorophyll a ratio and photosynthetic efficiency, and higher nonphotochemical quenching and saturation irradiance provided Pseudanabaena photoadaptation and photoprotection advantages over the two Microcystis strains under the high‐light condition. The lower investment in antenna pigments of Pseudanabaena than the two Microcystis strains under high‐light intensity is likely an efficient strategy for both saving iron quotas and decreasing photosensitivity. Therefore, when compared with Microcystis, the high plasticity of antenna pigments, along with the excellent photoadaptation and photoprotection ability of Pseudanabaena, probably ensures its ecological success under iron limitation when light is sufficient.

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Microcystis botrys and Lemmermanniella flexa - New Species of Cyanoprokaryota for the Flora of Ukraine in Phytoplankton of the Kanev Reservoir

Cited by 4 publications

References 0 publications

Obtaining Genome Sequences of Mutualistic Bacteria in Single Microcystis Colonies

Obtaining Genome Sequences of Mutualistic Bacteria in Single Microcystis Colonies

Temperature-Dependent Growth Characteristics and Competition of Pseudanabaena and Microcystis

Photosynthetic adaptation to light availability shapes the ecological success of bloom‐forming cyanobacterium Pseudanabaena to iron limitation

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