Microdevice for Separation of Circulating Tumor Cells using Embedded Magnetophoresis with V-shaped Ni-Co Nanowires and Immuno-nanomagnetic Beads

Park, Jeong Won; Lee, Nae-Rym; Cho, Sung Mok; Jung, Moon Youn; Ihm, Chunhwa; Lee, Dae-Sik

doi:10.4218/etrij.15.0114.0572

Cited by 17 publications

(20 citation statements)

References 35 publications

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“…2 . With the recent progress in Bio-MEMS (Bio-Micro-Electro-Mechanical System) technology, a wide variety of novel approaches such as acoustophoresis 32 , magnetophoresis 33 , thermophoresis 34 , dielectrophoresis (DEP) 29 , and optically induced-dielectrophoresis (ODEP) 35 have been proposed for the manipulation of biological substances (e.g., cells 36 , bacteria 37 , or DNA 38 ). Among them, DEP force-based cell manipulation has been widely adopted for various applications 29 39 .…”

Section: Resultsmentioning

confidence: 99%

Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model

Chiu

Chou

Huang

et al. 2016

Sci Rep

110

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Circulating tumour cells (CTCs) in a blood circulation system are associated with cancer metastasis. The analysis of the drug-resistance gene expression of cancer patients’ CTCs holds promise for selecting a more effective therapeutic regimen for an individual patient. However, the current CTC isolation schemes might not be able to harvest CTCs with sufficiently high purity for such applications. To address this issue, this study proposed to integrate the techniques of optically induced dielectrophoretic (ODEP) force-based cell manipulation and fluorescent microscopic imaging in a microfluidic system to further purify CTCs after the conventional CTC isolation methods. In this study, the microfluidic system was developed, and its optimal operating conditions and performance for CTC isolation were evaluated. The results revealed that the presented system was able to isolate CTCs with cell purity as high as 100%, beyond what is possible using the previously existing techniques. In the analysis of CTC gene expression, therefore, this method could exclude the interference of leukocytes in a cell sample and accordingly contribute to higher analytical sensitivity, as demonstrated in this study. Overall, this study has presented an ODEP-based microfluidic system capable of simply and effectively isolating a specific cell species from a cell mixture.

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Section: Resultsmentioning

confidence: 99%

Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model

Chiu

Chou

Huang

et al. 2016

Sci Rep

110

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“…Based on a principle similar to Kim et al, Park et al used ferromagnetic Ni-Co nanowires in a microdevice for MCF-7 cells. Under an external magnetic field, anti-EpCAM MNP-labeled MCF-7 cells were collected from whole blood with a purity of 93% (Park et al, 2015). Cho et al also used ferromagnetic wires together with two permanent magnets for separation of anti-EpCAM magnetic bead-conjugated MCF-7 cells from red blood cell (RBC) -lysed whole blood (CTC-μChip) (Cho et al, 2016).…”

Section: Review Of Magnetic Manipulation Applicationsmentioning

confidence: 99%

Magnetic Force-Based Microfluidic Techniques for Cellular and Tissue Bioengineering

Yaman

Anıl-İnevi

Özçivici

et al. 2018

Front. Bioeng. Biotechnol.

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Live cell manipulation is an important biotechnological tool for cellular and tissue level bioengineering applications due to its capacity for guiding cells for separation, isolation, concentration, and patterning. Magnetic force-based cell manipulation methods offer several advantages, such as low adverse effects on cell viability and low interference with the cellular environment. Furthermore, magnetic-based operations can be readily combined with microfluidic principles by precisely allowing control over the spatiotemporal distribution of physical and chemical factors for cell manipulation. In this review, we present recent applications of magnetic force-based cell manipulation in cellular and tissue bioengineering with an emphasis on applications with microfluidic components. Following an introduction of the theoretical background of magnetic manipulation, components of magnetic force-based cell manipulation systems are described. Thereafter, different applications, including separation of certain cell fractions, enrichment of rare cells, and guidance of cells into specific macro- or micro-arrangements to mimic natural cell organization and function, are explained. Finally, we discuss the current challenges and limitations of magnetic cell manipulation technologies in microfluidic devices with an outlook on future developments in the field.

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“…Here, we developed GenoCTC, a novel CTC-isolating device, which relies on microfluidics and lateral magnetophoresis for the efficient separation of CTCs, which is stimulated by a magnetic field gradient-based force. The bottom magnetophoresis system selectively and precisely isolates CTCs using specific antibody-coated magnetic microbeads, whereas the top microfluidic platform controls the flow rate of the sample [21]. We optimized and validated GenoCTC, by spiking healthy blood samples with cancer cells and found a notably high recovery rate of 80%, a separation rate of 77%, and a purity of 90%.…”

Section: Introductionmentioning

confidence: 99%

An Immune–Magnetophoretic Device for the Selective and Precise Enrichment of Circulating Tumor Cells from Whole Blood

Chelakkot¹,

Ryu²,

Kim

et al. 2020

Micromachines

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Here, we validated the clinical utility of our previously developed microfluidic device, GenoCTC, which is based on bottom magnetophoresis, for the isolation of circulating tumor cells (CTCs) from patient whole blood. GenoCTC allowed 90% purity, 77% separation rate, and 80% recovery of circulating tumor cells at a 90 μL/min flow rate when tested on blood spiked with epithelial cell adhesion molecule (EpCAM)-positive Michigan Cancer Foundation-7 (MCF7) cells. Clinical studies were performed using blood samples from non-small cell lung cancer (NSCLC) patients. Varying numbers (2 to 114) of CTCs were found in each NSCLC patient, and serial assessment of CTCs showed that the CTC count correlated with the clinical progression of the disease. The applicability of GenoCTC to different cell surface biomarkers was also validated in a cholangiocarcinoma patient using anti-EPCAM, anti-vimentin, or anti-tyrosine protein kinase MET (c-MET) antibodies. After EPCAM-, vimentin-, or c-MET-positive cells were isolated, CTCs were identified and enumerated by immunocytochemistry using anti-cytokeratin 18 (CK18) and anti-CD45 antibodies. Furthermore, we checked the protein expression of PDL1 and c-MET in CTCs. A study in a cholangiocarcinoma patient showed that the number of CTCs varied depending on the biomarker used, indicating the importance of using multiple biomarkers for CTC isolation and enumeration.

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Microdevice for Separation of Circulating Tumor Cells using Embedded Magnetophoresis with V-shaped Ni-Co Nanowires and Immuno-nanomagnetic Beads

Cited by 17 publications

References 35 publications

Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model

Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model

Magnetic Force-Based Microfluidic Techniques for Cellular and Tissue Bioengineering

An Immune–Magnetophoretic Device for the Selective and Precise Enrichment of Circulating Tumor Cells from Whole Blood

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