Microdialysis monitoring of variations in extracellular levels of serotonin, GABA and excitatory amino acids in the frontal cortex of awake rats in response to a single peripheral or central administration of dexfenfluramine

Rocher, Cyril; Bert, Lionel; Robert, F.; Trouvin, Jean‐Hugues; Renaud, Bernard; Jacquot, C.; Gardier, Alain M.

doi:10.1016/0006-8993(96)00735-4

Cited by 32 publications

(20 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This latter value is much higher than the expected concentration of 5-HT in brain microdialysates, which, as determined by LC, was found to be in the 5610 210 mol/L range in the rat [8,28]. In order to lower the LOD, an in-capillary preconcentration approach [29][30][31][32] was chosen.…”

Section: Optimization Of the Separation And Ph-mediated Preconcentrationmentioning

confidence: 91%

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

et al. 2005

Self Cite

View full text Add to dashboard Cite

Serotonin or 5-hydroxytryptamine (5-HT) is a major neurotransmitter in the central nervous system. In this work, a method for analyzing 5-HT in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. A pH-mediated in-capillary preconcentration of samples was performed, and after separation by capillary zone electrophoresis, native fluorescence of 5-HT was detected by a 266 nm solid-state laser. The separation conditions for the analysis of 5-HT in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical bases. Separation of 5-HT was performed using a 80 mmol/L citrate buffer, pH 2.5, containing 20 mmol/L hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and +30 kV voltage. The detection limit was 2.5 x 10(-10) mol/L. This method allows the in vivo brain monitoring of 5-HT using a simple, accurate CE measurement in underivatized microdialysis samples.

show abstract

Section: Optimization Of the Separation And Ph-mediated Preconcentrationmentioning

confidence: 91%

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…The effect of antihistamines on the metabolism of FEN to norFEN, however, remains to be established. Release of 5-HT by FEN and norFEN has been implicated in FEN-and norFEN-induced neurotoxicity since blockade of the endogeneous release and reuptake of 5-HT with 5-HT uptake inhibitors, fluoxetine and citalopram, attenuated FEN-induced neurotoxicity (Bergami et al, 1997;Bonanno et al, 1994;Clineschmidt et al, 1978;Fuller et al, 1975b;Rocher et al, 1996;Sabol et al, 1992). As mentioned before, induction of neurotoxicity of FEN, MDMA, and other 5-HT releasers may share similar mechanism.…”

Section: Possible Effect Of Antihistamines On Fen-induced Depletion Omentioning

confidence: 92%

Effects of antihistamines on fenfluramine-induced depletion of indoles in the brain of rats

Yeh¹

1999

Synapse

View full text Add to dashboard Cite

Various effects of chlorpheniramine (CPA), diphenhydramine (DIPH), tripelennamine (TRIP), and pyrilamine (PYRI) on fenfluramine (FEN)-induced depletion of serotonin in the brain of rats were observed to be dependent on body temperature. Levels of 5-HT and 5-HIAA in the frontal cortex, hippocampus, and striatum of rats treated with FEN (10 mg/kg, once or twice daily x 4 days) decreased to approximately 30% (P < 0.01) that of controls with no significant changes after CPA, DIPH, TRIP, and PYRI. Treatment with FEN plus CPA (5, 10, 20 mg/kg) and FEN plus DIPH (20 mg/kg), but not FEN plus TRIP (20 mg/kg) and FEN plus PYRI (20 mg/kg), increased brain serotonin levels 2- to 3-fold more than those treated with FEN plus saline. Treatment with FEN plus CPA and FEN plus DIPH, but not FEN plus TRIP and FEN plus PYRI, decreased rectal temperature with no significant change after FEN. The antihistamines alone decreased temperature at a 1-hour period and enhanced FEN-induced reduction in body weight. Possible mechanisms of the different effects of antihistamines on FEN-induced depletion of serotonin are discussed.

show abstract

“…In addition, the separation was quite rapid, as less than 85 s was required for the migration of Glu and Asp. These compounds were determined in the frontal cortex of awake rats in response to a single peripheral or central administration of dexfenfluramine (Rocher et al, 1996) and in the striatum and frontal cortex after subcutaneous injection of SB-271046, the selective 5-HT(6) receptor antagonist (Dawson et al, 2000). An improvement of the method allowed the separation of γ-aminobutyric acid (GABA), Glu and Asp in less than 10 min by the use of an SDS and hydroxypropyl-β-cyclodextrin buffer ( Fig.…”

Section: Labelling and Analysis By Capillary Electrophoresismentioning

confidence: 99%

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

Rammouz

Lacroix

Garrigues

et al. 2007

Biomedical Chromatography

View full text Add to dashboard Cite

This paper reviews analytical methods, instrumental developments and applications for derivatization of primary amines with naphthalene-2,3-dicarboxaldehyde using fluorescence and chemiluminescence detection with capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). The use of lasers as well as lamps as the excitation source for fluorescence detection is discussed. The detection limit observed with naphthalene-2,3-dicarboxaldehyde derivatization is often lower and better than those obtained with other analytical separations and other fluorescent dyes. In addition, this paper describes the crucial points that influence the stability of NDA primary amine derivatives, and summarize the separation, derivatization and migration conditions of the different techniques, with their advantages and drawbacks.

show abstract

Microdialysis monitoring of variations in extracellular levels of serotonin, GABA and excitatory amino acids in the frontal cortex of awake rats in response to a single peripheral or central administration of dexfenfluramine

Cited by 32 publications

References 43 publications

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

Effects of antihistamines on fenfluramine-induced depletion of indoles in the brain of rats

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

Contact Info

Product

Resources

About