Microdialysis of neuropeptide Y in human muscle tissue

Ernberg, Malin; Alstergren, Per

doi:10.1016/j.jneumeth.2003.09.009

Cited by 8 publications

(5 citation statements)

References 26 publications

(41 reference statements)

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“…22 The relative recovery of NPY in the kind of probes that we used has recently been found to be about 16% at a perfusion speed of 4 ml/min. 14 The increase in detectability of CGRP in the present study from mostly below the limit of detection at rest to clearly detectable concentrations after exercise probably represents a local release of CGRP in response to exercise.…”

Section: Discussionmentioning

confidence: 44%

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Calcitonin gene related peptide and neuropeptide Y in skeletal muscle after eccentric exercise: a microdialysis study

Jönhagen

Ackermann²,

Saartok³

et al. 2006

Br J Sports Med

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Section: Discussionmentioning

confidence: 44%

“…However, neuropeptides such as CGRP and NPY have been little studied in vivo in human skeletal muscle. 14 Microdialysis is a technique that can be used in vivo to measure tissue concentrations of different substances in human tissue. A thin plastic tube with a diameter of about 1 mm is introduced into the muscle with a thin needle.…”

mentioning

confidence: 99%

Calcitonin gene related peptide and neuropeptide Y in skeletal muscle after eccentric exercise: a microdialysis study

Jönhagen

Ackermann²,

Saartok³

et al. 2006

Br J Sports Med

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“…It was recently suggested that TNF has a central role in adult myogenesis and in maintenance of muscle homeostasis [ 74 ], and that it promotes muscle regeneration via expansion of muscle stem cells [ 75 ]. TNF is expressed in myoblasts and a rapid increase of TNF expression takes place during the early hours of differentiation [ 76 ]. Thus, one could speculate that the release of TNF is blunted in skeletal muscles of patients with FM due to sustained muscle activity (between muscle contractions) leading to or reflecting an impaired muscle regeneration, and that this ultimately may reduce muscle tissue quality.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Levels of Pro-Inflammatory Cytokines Released in the Vastus Lateralis Muscle of Patients with Fibromyalgia and Healthy Controls during Contractions of the Quadriceps Muscle – A Microdialysis Study

et al. 2015

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ObjectiveFibromyalgia is associated with central hyperexcitability, but it is suggested that peripheral input is important to maintain central hyperexcitability. The primary aim was to investigate the levels of pro-inflammatory cytokines released in the vastus lateralis muscle during repetitive dynamic contractions of the quadriceps muscle in patients with fibromyalgia and healthy controls. Secondarily, to investigate if the levels of pro-inflammatory cytokines were correlated with pain or fatigue during these repetitive dynamic contractions.Material and Methods32 women with fibromyalgia and 32 healthy women (controls) participated in a 4 hour microdialysis session, to sample IL-1β, IL-6, IL-8, and TNF from the most painful point of the vastus lateralis muscle before, during and after 20 minutes of repeated dynamic contractions. Pain (visual analogue scale; 0–100) and fatigue Borg’s Rating of Perceived Exertion Scale; 6–20) were assessed before and during the entire microdialysis session.ResultsThe repetitive dynamic contractions increased pain in the patients with fibromyalgia (P < .001) and induced fatigue in both groups (P < .001). Perceived fatigue was significantly higher among patients with fibromyalgia than controls (P < .001). The levels of IL-1β did not change during contractions in either group. The levels of TNF did not change during contractions in patients with fibromyalgia, but increased in controls (P < .001) and were significantly higher compared to patients with fibromyalgia (P = .033). The levels of IL-6 and IL-8 increased in both groups alike during and after contractions (P’s < .001). There were no correlations between pain or fatigue and cytokine levels after contractions.ConclusionThere were no differences between patients with fibromyalgia and controls in release of pro-inflammatory cytokines, and no correlations between levels of pro-inflammatory cytokines and pain or fatigue. Thus, this study indicates that IL-1β, IL-6, IL-8, and TNF do not seem to play an important role in maintenance of muscle pain in fibromyalgia.

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“…[14][15][16] Since then, commercially available polyethersulfone microdialysis probes with an MWCO of 6000 to 100 000 Da have been used to recover a wide range of molecular species ranging in size from 3000 to 120 000 Da. [17][18][19][20] Some of these studies are summarized in Table 1 .…”

Section: Principles Of Dialysis Of Large Moleculesmentioning

confidence: 99%