Microdialysis of proteins: Performance of the CMA/20 probe

Rosenbloom, Alan J.; Sipe, David M.; Weedn, Victor W.

doi:10.1016/j.jneumeth.2005.04.018

Cited by 44 publications

(57 citation statements)

References 15 publications

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“…In conditions where there is a decrease in both cerebral tissue glucose and PtiO2, a reduction of capillary blood flow may be inferred [63,64] . Lactate studies can indicate the intensity of anaerobic metabolism, while glycerol studies can evaluate tissue damage since glycerol is one of the structural components in the tissue lipid layer of cell membranes [66] . Glutamate is an important excitatory neurotransmitter in the mammalian nervous system, with aspartate following in importance.…”

Section: Metabolic Monitoringmentioning

confidence: 99%

“…In situations of excitotoxicity, massive release of glutamate into the synaptic cleft can be seen. Thus, large inputs of calcium into the cell are observed; as a consequence, there is production of oxygen free radicals in cell membranes and the release of more fatty acids and glycerol [66] . It is recommended that the MD catheter be placed in socalled "penumbra" areas adjacent to focal lesions in order to allow monitoring of potentially recoverable brain regions [68,69] .…”

Section: Metabolic Monitoringmentioning

confidence: 99%

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Multimodal brain monitoring in fulminant hepatic failure

Paschoal¹,

Nogueira²,

Ronconi³

et al. 2016

WJH

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Acute liver failure, also known as fulminant hepatic failure (FHF), embraces a spectrum of clinical entities characterized by acute liver injury, severe hepatocellular dysfunction, and hepatic encephalopathy. Cerebral edema and intracranial hypertension are common causes of mortality in patients with FHF. The management of patients who present acute liver failure starts with determining the cause and an initial evaluation of prognosis. Regardless of whether or not patients are listed for liver transplantation, they should still be monitored for recovery, death, or transplantation. In the past, neuromonitoring was restricted to serial clinical neurologic examination and, in some cases, intracranial pressure monitoring. Over the years, this monitoring has proven insufficient, as brain abnormalities were detected at late and irreversible stages. The need for real-time monitoring of brain functions to favor prompt treatment and avert irreversible brain injuries led to the concepts of multimodal monitoring and neurophysiological decision support. New monitoring techniques, such as brain tissue oxygen tension, continuous electroencephalogram, transcranial Doppler, and cerebral microdialysis, have been developed. These techniques enable early diagnosis of brain hemodynamic, electrical, and biochemical changes, allow brain anatomical and physiological monitoring-guided therapy, and have improved patient survival rates. The purpose of this review is to discuss the multimodality methods available for monitoring patients with FHF in the neurocritical care setting.

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Section: Metabolic Monitoringmentioning

confidence: 99%

Section: Metabolic Monitoringmentioning

confidence: 99%

Multimodal brain monitoring in fulminant hepatic failure

Paschoal¹,

Nogueira²,

Ronconi³

et al. 2016

WJH

View full text Add to dashboard Cite

show abstract

“…The sampling order were randomly chosen (Table S1) and could therefore not explain the shape of the curves. It is also known that there could be large Page | 18 variation in EE between different probes (Galea et al 2014) and in sampling time (Rosenbloom et al 2005). Therefore, MD dialysate fractions from four probes and at least two sampling times were pooled into one MS-sample, meaning that a specific probe at a certain time only contributed to 8-12% of the total result.…”

Section: Extraction Efficiency Of Different Proteins and Peptidesmentioning

confidence: 99%

“…Albumin will also diffuse through a 100 kDa membrane, mix into the tissue and contaminate the sample environment. Compared to albumin, dextrans of higher MWs will not diffuse through a 100 kDa membrane and are compatible with LC/MS (Rosenbloom et al 2005). …”

Section: Introductionmentioning

confidence: 99%

Influence of surface modification and static pressure on microdialysis protein extraction efficiency

Chu

Undin

Lind

et al. 2015

Biomed Microdevices

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Influence of surface modification and static pressure on microdialysis protein extractionefficiency. Biomedical microdevices (Print)http://dx.doi.org/10.1007/s10544-015-0005-3Access to the published version may require subscription. N.B. When citing this work, cite the original published paper. ABSTRACTThere is growing interest in using microdialysis (MD) for monitoring larger and more complex molecules such as neuropeptides and proteins. This promotes the use of MD membranes with molecular weight cut off (MWCO) of 100 kDa or above. The hydrodynamic property of the membrane goes to ultrafiltration or beyond, making the MD catheters more sensitive to pressure.In the meantime, despite the large pore size, studies have shown that membrane biofouling still lead to unstable catheter performance. The objective is to study in vitro how 500 kDa dextran and Poloxamer 407 surface modification affect the fluid recovery (FR) and extraction efficiency (EE) of 100 kDa MWCO MD catheters. A pressure chamber was designed to facilitate the tests, using as MD sample a protein standard with similar concentrations as in human cerebral spinal fluid, comparing native and Poloxamer 407 modified MD catheters. The collected dialysate fractions were examined for FR and protein EE, employing Dot-it Spot-it Protein Assay for total protein EE and targeted mass spectrometry (MS) for EE of individual proteins and peptides. The FR results suggested that the surface modified catheters were less sensitive to the pressure and provide higher precision, and provided a FR closer to 100%. The surface modification did not show a significant effect on the protein EE. The average total protein EE of surface modified catheters was slightly higher than that of the native ones. The MS EE data of individual proteins showed a clear trend of complex response in EE with pressure.

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“…Contudo, uma microdiálise de proteínas maiores exige taxas mais lentas de fluxo tampão, grandes sondas de poros e atenção para a perda de água a partir da sonda. O cateter de microdiálise pode ser colocado no parênquima cerebral encapsulado sob o couro cabeludo por meio de uma craniotomia local, por um orifício de trepanação ou diretamente por um parafuso fixo ao crânio (Rosenbloom et al, 2005).…”

Section: Espectroscopia De Infravermelho Próximounclassified

Autorregulação encefálica na insuficiência hepática fulminante antes e após transplante hepático

Júnior¹,

Mendes²

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Microdialysis of proteins: Performance of the CMA/20 probe

Cited by 44 publications

References 15 publications

Multimodal brain monitoring in fulminant hepatic failure

Multimodal brain monitoring in fulminant hepatic failure

Influence of surface modification and static pressure on microdialysis protein extraction efficiency

Autorregulação encefálica na insuficiência hepática fulminante antes e após transplante hepático

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