Microdilution Technique for Antimicrobial Susceptibility Testing of Anaerobic Bacteria

Abstract

A microdilution technique using commercially available media and materials was developed and used to determine the minimal inhibitory concentrations (MICs) of clindamycin, chloramphenicol, tetracycline, minocycline, ampicillin, carbenicillin, cephalothin, and gentamicin for 101

Synergistic Action of Erythromycin and Cefamandole Against Bacteroides fragilis Subsp. fragilis

“…After inoculation, the plates were sealed with plastic tape and incubated in the anaerobic glove box at 37°C for 24 h. After incubation the plates were read with the Autotray Viewer (Ames Co.) (14,17). The addition of L-cysteine, prereducing the medium by autoclaving, and storage in the controlled atmosphere of the glove box made it possible to read the results in 24 instead of 48 h.…”

Simplified Method for Antimicrobial Susceptibility Testing of Anaerobic Bacteria

“…In a previous study (10), 8% of strains did not grow by day 1 and 7% of the MICs for those that did grow were two or more concentrations lower than the 2-day MICs. Two-day MICs were reproducible, and unexpectedly high MICs after 2 days of incubation due to breakthrough growth did not seem to occur.…”

“…MICs were determined in an anerobic glove box by a microdilution method (10). Each of the four antibiotics studied was added to the first two wells of two horizontal rows in the microdilution plate, and serial twofold dilutions were made from the second set of wells.…”

“…Results are compared to minimal inhibitory concentrations which were determined simultaneously by using a microdilution method in an anaerobic glove box. Twenty strains of Bacteroides fragilis, 10 Antimicrobial susceptibility testing of anaerobic bacteria is usually done by agar or broth dilution methods (16). Disk diffusion methods (2,3,6,8,11,14,15,17,19), analogous to that described by Bauer et al (1) for rapidly growing aerobic and facultative bacteria, have also been developed in attempts to simplify and standardize the approach to antimicrobial susceptibility testing of anaerobes.…”

Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

“…The antibacterial activity was determined by the microdilution method using a 96-well plate. 15 The inoculate was prepared by suspending 10 5 colony-forming units/mL of cells in sterile Luria-Bertani medium. After reaching confluence, cells were treated with appropriate amounts of the synthesized nanoparticles.…”