Microdissection: A method developed to investigate mechanisms involved in transmissible spongiform encephalopathy pathogenesis

Barr, J.; Somerville, Robert A.; Chung, Yuen‐Li; Fraser, James E.

doi:10.1186/1471-2334-4-8

Cited by 11 publications

(12 citation statements)

References 23 publications

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“…Additionally, affected hosts show neuropathological characteristics including spongiform vacuolation, neuronal loss, astrogliosis, and deposition of PrP Sc in the brain tissue [33]. Occasionally, PrP Sc deposits are found as large rod-like amyloid plaques [34].…”

Section: Prion Diseasementioning

confidence: 99%

Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion

Kang

Rahim

et al. 2017

BioMed Research International

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Prions composed of pathogenic scrapie prion protein (PrPSc) are infectious pathogens that cause progressive neurological conditions known as prion diseases or transmissible spongiform encephalopathies. Although these diseases pose considerable risk to public health, procedures for early diagnosis have not been established. One of the most recent attempts at sensitive and specific detection of prions is the real-time quaking-induced conversion (RT-QuIC) method, which measures the activity of PrPSc aggregates or amyloid formation triggered by PrPSc seeds in the presence of recombinant PrP. In this review, we summarize prions, prion diseases, and current approaches to diagnosis, including the principle, conditions for assay performance, and current diagnostic applications of RT-QuIC.

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Section: Prion Diseasementioning

confidence: 99%

Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion

Kang

Rahim

et al. 2017

BioMed Research International

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“…Central nervous system tissue was collected from all animals after anaesthesia and perfusion using a peristaltic pump with a blunt cannula inserted into the aortic artery (fixative solution containing 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4). Then, we trimmed the brain into four different sections following the scheme previously described [41]. The sections were paraffin‐embedded and processed into 4 µm thickness.…”

Section: Methodsmentioning

confidence: 99%

Consequences of dietary manganese and copper imbalance on neuronal apoptosis in a murine model of scrapie

Bolea

Hortells

Martín-Burriel

et al. 2010

Neuropathology Appl Neurobio

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With regard to apoptosis induction, the response of Tga20 mice to prion infection was similar to that reported for other mice models. Our results demonstrate the neuroprotective effects of -Cu, -Cu+Mn and +Mn diets in a murine model of scrapie. However, neuronal death induced by infection with prions seems to be independent of apoptosis marker signalling. Moreover, copper-modified diets were neuroprotective against the possible toxicity of the prion transgene in Tga20 control and infected mice even though manganese supplementation could not counteract this toxicity.

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“…The tissues were then cryoprotected by immersion in 0.1 m phosphate buffer containing 30% sucrose and stored at 4°C (Rodrigo et al., 1996). Finally, they were trimmed following the scheme previously described by Barr et al. (2004).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Metal Imbalances on Scrapie Neurodegeneration

Hortells¹,

Monleón²,

Acín³

et al. 2009

Zoonoses and Public Health

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Environmental exposure to metal appears to enhance susceptibility to Transmissible Spongiform Encephalopathies (TSEs); however, published data are not conclusive. The current study focuses on assessing the effects of copper depletion and/or manganese enhancement in the diet on susceptibility to Scrapie and this disease progression. The degree of spongiosis was the highest in the animals that received a copper- depleted diet. These observations suggest that this diet contributes to the Scrapie lesions and to the worsening of the condition in animals that have been inoculated with Scrapie. The highest intensities of GFAP immunostaining were also associated with the copper- depleted diet. Dietary supplementation with manganese had a negative effect on neuronal counts. In conclusion, this study demonstrates that certain environmental factors may aggravate neuropathological Scrapie lesions. This is consistent with reports from other neurodegenerative diseases where some metalloenzymes play a pivotal protector role against the oxidative stress associated with pathogenesis.

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Microdissection: A method developed to investigate mechanisms involved in transmissible spongiform encephalopathy pathogenesis

Cited by 11 publications

References 23 publications

Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion

Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion

Consequences of dietary manganese and copper imbalance on neuronal apoptosis in a murine model of scrapie

The Effect of Metal Imbalances on Scrapie Neurodegeneration

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