Microdissection Methods Utilizing Single-Cell Subtype Analysis and the Impact on Precision Medicine

Johann, Donald J.; Laun, Sarah; Shin, Ik Jae; Weigman, Robert; Stephens, Owen; Roberge, Adam; Liu, Meeiyueh; Greisman, Valerie; Steliga, Matthew A.; Muesse, Jason; Peterson, Erich A.; Emmert‐Buck, Michael R.; Tangrea, Michael A.

doi:10.1007/978-1-0716-1811-0_7

Cited by 1 publication

(2 citation statements)

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“…Several institutions, including the Mayo Clinic, currently employ LCM to enrich for amyloidogenic proteins before further analysis with mass spectrometry on the polypeptides [69, 70]. While LCM remains popular in basic science research, forays into clinical practice, such as digital droplet PCR and RNA-seq for clinically relevant gene expression tests, are on the horizon [71, 72].…”

Section: Microdissection Methodsmentioning

confidence: 99%

“…The development of uncross-linked membranes by our lab has led to significant increases in DNA and miRNA yields (unpublished data). Additionally, antibodies and other molecular tags do not currently exist for every analyte of interest, which makes this technique inapplicable in several cases [66,71]. Since xMD is still in development, there are few published studies that incorporate it outside of optimization protocols.…”

Section: Expression Microdissectionmentioning

confidence: 99%

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A Comparison of Tissue Dissection Techniques for Diagnostic, Prognostic, and Theragnostic Analysis of Human Disease

Walsh

Halushka

2022

Pathobiology

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Histopathology has historically been the critical technique for the diagnosis and treatment of human disease. Today, genomics, transcriptomics, and proteomics from specific cells, rather than bulk tissue, have become key to understanding underlying disease mechanisms and rendering useful diagnostic information. Extraction of desired analytes, i.e., nucleic acids or proteins, from easily accessible formalin-fixed paraffin-embedded tissues allows for clinically relevant activities, such as sequencing biomarker mutations or typing amyloidogenic proteins. Genetic profiling has become routine for cancers as varied as non-small cell lung cancer and prostatic carcinoma. The five main tissue dissection techniques that have been developed thus far include: bulk scraping, manual macrodissection, manual microdissection, laser-capture microdissection, and expression microdissection. In this review, we discuss the importance of tissue dissection in clinical practice and research, the basic methods, applications, as well as some advantages and disadvantages for each modality.

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