Microdissection of mouse and human zona pellucida using a 1.48µm diode laser beam: efficacy and safety of the procedure

Purpose A laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development. Methods Each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III). Results For groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0±0.0), II (1.0±0.2), and III (1.1±0.2) was significant (0.0001). Conclusion Our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.

Section: Discussionsupporting

confidence: 89%

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

Taylor

Gilchrist

Hallowell

et al. 2010

“…We first evaluated the impact of laser drilling and polar body biopsy on oocyte lysis. As previously described [30], we did not find any postprocedure increase in lysis rate (3 oocytes lysed in the whole population). This result was in accordance with prospective, non-randomized studies where lysis rates were very low after treatment [5,7], but also in accordance to ICSI lysis rate [42], procedure considered as safe for oocytes.…”

Section: Discussionsupporting

confidence: 77%

“…Acid Tyrode's solution is still the most widely used method, but laser zona drilling and thinning are increasingly used. Since the first reported laser systems for zona pellucida micromanipulation [29], many systems have been developed and the 1.48 μm diode non-contact infrared laser is now considered as the most acceptable for laser zona drilling, because it does not use UV lasers, which are associated with mutagenic change in DNA [30].…”

Section: Introductionmentioning

confidence: 99%

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?

Hammoud

Molina-Gomès

Albert

et al. 2010

Introduction Preconception diagnosis requires first polar body biopsy. When the hole in the zona pellucida is made with a laser beam, heat propagation could, like the biopsy itself, be deleterious. Our aim was to evaluate the effect of this technique on human in vitro matured oocyte and embryo development. Methods One hunded fifty five retrieved immature oocytes from 75 women, matured in vitro, were distributed in 3 groups: 50 oocytes in a control group, without laser drilling and first polar body biopsy, 52 oocytes in a group with only laser drilling, and 53 oocytes in a group with both laser drilling and first polar body biopsy. Safety was evaluated using four criteria: [1] oocyte lysis rate, [2] oocyte activation rate, [3] oocyte development after calcium ionophore treatment, [4] and embryo chromosome breakage incidence after Tarkowski preparation. Results No difference in the four criteria was observed between the 3 oocyte groups.Conclusions We did not find evidence of deleterious effect of laser drilling and first polar body biopsy on in vitro matured oocytes, according to our criteria.

“…Mechanical ZP dissection with glass pipettes [7] or chemical ZP opening using acidic Tyrode's solution [8,9] are in wide use. Recently, AH by 1.48 μm diode laser has been introduced and has enabled to reliably produce equally sized ZP opening [10][11][12][13]. However, studies evaluating the effect of the size of ZP opening by laser AH for frozen cleaved embryos are lacking.…”

Section: Introductionmentioning

confidence: 99%

Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study

Fuchiwaki

Hiraoka

Horiuchi

et al. 2008