Microdochium majus and other fungal pathogens associated with reduced gluten quality in wheat grain

Aamot, Heidi Udnes; Lysøe, Erik; Koga, Shiori; Nielsen, Katherine Ann Gredvig; Böcker, Ulrike; Brodal, Guro; Dill‐Macky, Ruth; Uhlen, Anne Kjersti; Hofgaard, Ingerd Skow

doi:10.1016/j.ijfoodmicro.2020.108712

Cited by 10 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The M. nivale assay was a SYBR Green assay, all others were probe assays. The qPCR reactions were performed according to Aamot et al [ 47 ], in a total volume of 25 µL that consisted of 4 µL genomic DNA from wheat samples (diluted 1 + 9 with PCR grade water) or DNA from pure cultures (standards). Assays for Fg, Fp and Mm were probe assays that were run in singleton reactions, and included 300 nM of each primer, 100 nM probe and Sso Advanced™ Universal Probes Supermix (Bio-Rad, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Different Resistance to DON versus HT2 + T2 Producers in Nordic Oat Varieties

Hofgaard

Brodal

Almvik

et al. 2022

Toxins

Self Cite

View full text Add to dashboard Cite

Over recent decades, the Norwegian cereal industry has had major practical and financial challenges associated with the occurrence of Fusarium head blight (FHB) pathogens and their associated mycotoxins in cereal grains. Deoxynivalenol (DON) is one of the most common Fusarium-mycotoxins in Norwegian oats, however T-2 toxin (T2) and HT-2 toxin (HT2) are also commonly detected. The aim of our study was to rank Nordic spring oat varieties and breeding lines by content of the most commonly occurring Fusarium mycotoxins (DON and HT2 + T2) as well as by the DNA content of their respective producers. We analyzed the content of mycotoxins and DNA of seven fungal species belonging to the FHB disease complex in grains of Nordic oat varieties and breeding lines harvested from oat field trials located in the main cereal cultivating district in South-East Norway in the years 2011–2020. Oat grains harvested from varieties with a high FHB resistance contained on average half the levels of mycotoxins compared with the most susceptible varieties, which implies that choice of variety may indeed impact on mycotoxin risk. The ranking of oat varieties according to HT2 + T2 levels corresponded with the ranking according to the DNA levels of Fusarium langsethiae, but differed from the ranking according to DON and Fusarium graminearum DNA. Separate tests are therefore necessary to determine the resistance towards HT2 + T2 and DON producers in oats. This creates practical challenges for the screening of FHB resistance in oats as today’s screening focuses on resistance to F. graminearum and DON. We identified oat varieties with generally low levels of both mycotoxins and FHB pathogens which should be preferred to mitigate mycotoxin risk in Norwegian oats.

show abstract

Section: Methodsmentioning

confidence: 99%

Different Resistance to DON versus HT2 + T2 Producers in Nordic Oat Varieties

Hofgaard

Brodal

Almvik

et al. 2022

Toxins

Self Cite

View full text Add to dashboard Cite

show abstract

“…109 Conversely, Microdochium, a well-known head-blight agent in wheat, 110 even if a non-toxigenic genus, can affect grain gluten quality. 111 Finally, Aspergillus and Penicillium, other mycotoxigenic genera, were also found. This highlights that the fungal communities associated with durum wheat grains are a reservoir of pathogenic (toxigenic and non-toxigenic) as well as endophytic non-pathogenic fungal genera, which could compete with or prevent FHB and other diseases, representing a source of potential biocontrol agents in wheat.…”

Section: Discussionmentioning

confidence: 99%

“…The wheat endophytic Acremonium species showed mutual exclusion relationship in wheat with the pathogenic genus Puccinia 109 . Conversely, Microdochium , a well‐known head‐blight agent in wheat, 110 even if a non‐toxigenic genus, can affect grain gluten quality 111 . Finally, Aspergillus and Penicillium , other mycotoxigenic genera, were also found.…”

Section: Discussionmentioning

confidence: 99%

Different diagnostic approaches for the characterization of the fungal community and Fusarium species complex composition of Italian durum wheat grain and correlation with secondary metabolite accumulation

et al. 2023

View full text Add to dashboard Cite

BACKGROUND The evolution of the fungal communities associated with durum wheat was assessed using different diagnostic approaches. Durum wheat grain samples were collected in three different Italian cultivation macro‐areas (north, center and south). Fungal isolation was realized by potato dextrose agar (PDA) and by deep‐freezing blotter (DFB). Identification of Fusarium isolates obtained from PDA was achieved by partial tef1α sequencing (PDA + tef1α), while those obtained from DFB were identified from their morphological characteristics (DFB + mc). The fungal biomass of eight Fusarium species was quantified in grains by quantitative polymerase chain reaction (qPCR). Fungal secondary metabolites were analyzed in grains by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Correlations between Fusarium detection techniques (PDA + tef1α; DFB + mc and qPCR) and mycotoxins in grains were assessed. RESULTS Alternaria and Fusarium showed the highest incidence among the fungal genera developed from grains. Within the Fusarium community, PDA + tef1α highlighted that F. avenaceum and F. graminearum were the most represented members, while, DFB + mc detected a high presence of F. proliferatum. Alternaria and Fusarium mycotoxins, principally enniatins, were particularly present in the grain harvested in central Italy. Deoxynivalenol was mainly detected in northern‐central Italy. CONCLUSIONS The adoption of the different diagnostic techniques of Fusarium detection highlighted that, for some species, qPCR was the best method of predicting their mycotoxin contamination in grains. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

show abstract

“…Recently, some gluten-degrading bacteria and gluten-degrading enzymes have been discovered and studied on the detoxification of gluten, such as Fusarium graminearum [10], Microdochium majus [11], Bacillus spp. [12], actinidin from kiwifruit [13], and a serine protease from Burkholderia gladioli [14].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Bacillus cereus AFA01 Capable of Degrading Gluten and Celiac-Immunotoxic Peptides

Lü

Yuan

et al. 2021

Foods

View full text Add to dashboard Cite

Wheat gluten elicits a pro-inflammatory immune response in patients with celiac disease. The only effective therapy for this disease is a life-long gluten-free diet. Gluten detoxification using glutenases is an alternative approach. A key step is to identify useful glutenases or glutenase-producing organisms. This study investigated the gluten-degrading activity of three Bacillus cereus strains using gluten, gliadin, and highly immunotoxic 33- and 13-mer gliadin peptides. The strain AFA01 was grown on four culture media for obtaining the optimum gluten degradation. Complete genome sequencing was performed to predict genes of enzymes with potential glutenase activity. The results showed that the three B. cereus strains can hydrolyze gluten, immunotoxic peptides, and gliadin even at pH 2.0. AFA01 was the most effective strain in degrading the 33-mer peptide into fractions containing less than nine amino acid residues, the minimum peptide to induce celiac responses. Moreover, growth on starch casein broth promoted AFA01 to degrade immunotoxic peptides. PepP, PepX, and PepI may be responsible for the hydrolysis of immunotoxic peptides. On the basis of the potential of gluten degradation, AFA01 or its derived enzymes may be the best option for further research regarding the elimination of gluten toxicity.

show abstract

Microdochium majus and other fungal pathogens associated with reduced gluten quality in wheat grain

Cited by 10 publications

References 47 publications

Different Resistance to DON versus HT2 + T2 Producers in Nordic Oat Varieties

Different Resistance to DON versus HT2 + T2 Producers in Nordic Oat Varieties

Different diagnostic approaches for the characterization of the fungal community and Fusarium species complex composition of Italian durum wheat grain and correlation with secondary metabolite accumulation

Characterization of Bacillus cereus AFA01 Capable of Degrading Gluten and Celiac-Immunotoxic Peptides

Contact Info

Product

Resources

About