The quality factor for x-rays, gamma rays and electrons assigned as one need to be revised. It is observed that as the energy decreases, mean lethal radiation dose (Do)decreases as well and become more potent. It is therefore proposed that radiation quality in biological systems should be assessed in the mitotic phase of the cell cycles. Furthermore, based on the mean lethal radiation dose within specific energy range, an appropriate quality factor of x-rays, gamma rays and electrons should be assigned.