Microelectrodes from PEDOT-carbon nanofiber composite for high performance neural recording, stimulation and neurochemical sensing

Abstract

This present method describes a versatile approach for the electrochemical synthesis of a composite material of Poly (3,4-ethylenedioxythiophene) (PEDOT) and Carbon Nanofibers (CNFs) for neural interfaces and biosensing applications. Oxidized CNFs were utilized as dopants of PEDOT to prepare the composite coating through electrochemical deposition on microelectrodes arrays (MEA). The experimental results of this study showed that PEDOT:CNF microelectrodes exhibit remarkable electrochemical properties, combining low impedance, high surface area, high charge injection capability and reliable neurotransmitters monitoring using amperometric techniques. Taken together, these results suggest the great potential of PEDOT:CNF composite for developing next-generation multifunctional microelectrodes for applications in neural therapies. A simple approach for the electrochemical synthesis of PEDOT:CNF composite material on microelectrodes for neural interfaces and neurochemical sensing. PEDOT:CNF microelectrodes exhibit remarkable electrochemical properties, combining low impedance and high charge injection capabilities. PEDOT:CNF microelectrodes allowed the reliable detection of neurotransmitters with improved sensitivity.

Scalable batch fabrication of ultrathin flexible neural probes using a bioresorbable silk layer

“…EIS and cyclic voltammetry (CV) were performed using a Bio-Logic VMP3 potentiostat. CV was performed in PBS at room temperature by potential sweeping between −0.6 and +0.6 V at 200 mV/s vs. Ag/ AgCl reference, allowing cathodal charge storage capacity (CSCc) evaluation 37,38 . EIS was also performed in PBS at room temperature by applying a 10 mV sine wave at frequencies ranging from 10 Hz to 7 MHz.…”

Nanofibrous PEDOT-Carbon Composite on Flexible Probes for Soft Neural Interfacing

“…Raw CNFs (Pyrograf ® -III, PR-19-XT-PS, pyrolytically stripped, platelets conical, >98% carbon basis, 20-200 µm) were oxidized using wet chemical oxidation process where 300 mg of CNFs were placed in 200 ml of the oxidizing solution (15 M) HNO 3 /(18 M) H 2 SO 4 and sonicated for 15 min, followed by 2 h of reflux at 70 °C (Rasheed et al, 2007;Bortolamiol et al, 2014). This process helps to remove impurities (metallic particles and amorphous carbon) from the sample and make them hydrophilic, so that the CNFs can be dispersed in water (Saunier et al, 2020a). Following the acid treatment, the CNF dispersion was washed with DI water to reach a neutral pH, and stored at 4 °C.…”

Scalable Batch Fabrication of Ultrathin Flexible Neural Probes using Bioresorbable Silk Layer

“…Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) methods were performed using a Bio-Logic VMP3 potentiostat. CV was performed in Phosphate Buffered Saline (Gibco DPBS 1X) at room temperature by potential sweeping between -0.6 V and +0.6 V vs. Ag/AgCl reference at 200 mV/s, allowing cathodal Charge Storage Capacity (CSCc) evaluation [34][35] . EIS was also performed in PBS at room temperature by applying 10 mV sine wave at frequencies ranging from 10 Hz to 7 MHz.…”