Microencapsulation of essential thyme oil by spray drying and its antimicrobial evaluation against Vibrio alginolyticus and Vibrio parahaemolyticus

Abstract

The aims of this research were first, to evaluate the antibacterial potential of commercial thyme essential oil against V. alginolyticus and V. parahaemolyticys and second, using the spray drying technique to produce microcapsules. chemical compounds of thyme oil and microcapsules were identified and quantified being thymol the chemical component present at the highest concentration. Oil-in-water (O/W) emulsions were prepared and the microcapsules were obtained with a spray dryer using maltodextrin as wall material (ratio 1:4). Thyme oil and the microcapsules exhibited antimicrobial activity against V. parahaemolyticus and V. alginolyticus. The spray drying process did not affect the antimicrobial activity of thyme essentialoil.Keywords: essential oil, Thymus vulgaris, Thymus zygis, bacterial control, aquaculture.Microencapsulação do óleo essencial de tomilho e avaliação de sua atividade antibacteriana sobre V. alginolyticus e V. parhaemolyticus ResumoOs objetivos desta pesquisa foram avaliar o potencial antibacteriano do óleoessencial de tomilho sobre V. alginolyticus e V. parahaemolyticys e produzir microcápsulas através do processo de secagem por aspersão (spray dryer). Os compostos químicos do óleo essencial de tomilho e das microcápsulas foram identificados e quantificadaos. Foi preparada uma emulsão de óleo em água (O/A) e em seguida foram produzidas microcápsulas em um spray dryer utilizando-se óleo essencial de tomilho e maltodextrina como material de parede na proporção de 1:4 respectivamente. Entre os vários compostos identificados, o timol apresentou maior concentração. O óleo essencial de tomilho e as microcápsulas exibiram atividade antibacteriana sobre V. parahaemolyticus e V. alginolyticus. O processo de secagem por aspersão não afetou a atividade antibacteriana do óleo essencial de tomilho.Palavras-chave: óleo essencial, Thymus vulgaris, Thymus zygis, controle bacteriano, aquicultura.

Influences of freeze‐ and spray‐drying vs. encapsulation with soy and whey proteins on gastrointestinal stability and antioxidant activity of Mediterranean aromatic herbs

“…On the other hand, the same phenolic compounds were identified as dominant in thyme freeze‐dried herbal material headspace (23.3% thymol, 13.4% carvacrol), but in spray‐dried thyme headspace, the percentage of both compounds significantly increased (37.0% thymol, 21.4% carvacrol), in accordance with the study of Tomazelli Junior et al . (2018). It is evident that two modern plant‐drying techniques (freeze‐ and spray‐drying) exhibited significant influence on the headspace profile and the abundance headspace compounds depended not only on the used drying technique but also on the influence of plant matrix.…”

Nanoencapsulation of thyme essential oil: a new avenue to enhance its protective role against oxidative stress and cytotoxicity of zinc oxide nanoparticles in rats

“…Then, essential oils were progressively added to the polymer solution with homogenization at 20000 rpm for 10 min to form an emulsion. The polymer concentration was 20%, and the amount of essential oil used was 10% of the mass of the polymer concentration (Tomazelli Júnior et al 2018 ). The emulsion solution was encapsulated by spray drying using a spray drier (B-290, Buchi ) equipped with a pressure air atomizing nozzle at 2.5 bar, inlet temperature of 160 ± 5°C, and outlet air temperature of 85 ± 5°C.…”

Encapsulation of Essential Oils - A Booster to Enhance their Bio-efficacy as Botanical Preservatives

“…Moreover, rapid and cost effective property of spray drying technique make it more preferable to be used in food industries for encapsulating food additives and enhancement in their shelf life. Microcapsules of thyme EO obtained by spray drying technique exhibited improved chemical stability and antimicrobial potential against Vibrio alginolyticus and V. parahaemolyticus (Tomazelli et al, 2018).…”