Microencapsulation of hemoglobin in liposomes using a double emulsion, film dehydration/rehydration approach

Zheng, S.; Zheng, Yaoming; Beissinger, Richard L.; Fresco, Raoul

doi:10.1016/0005-2736(94)00212-6

Cited by 24 publications

(31 citation statements)

References 32 publications

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“…This result is contradictory to Ref. [11], where DE, as a method, has been found to have higher encapsulation efficiency for hemoglobin than TFH. In our case, comparatively lower encapsulation efficiency for DE might be due to the loss of lauric acid coating during emulsion processes leading to aggregation of magnetic manganese ferrite and precipitation of particles before liposomal encapsulation.…”

Section: Resultscontrasting

confidence: 56%

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Preparation and characterization of manganese ferrite-based magnetic liposomes for hyperthermia treatment of cancer

Pradhan¹,

Giri

Banerjee³

et al. 2007

Journal of Magnetism and Magnetic Materials

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Section: Resultscontrasting

confidence: 56%

“…DE was performed following a reported method [11]. In brief, the required amount of egg-PC and cholesterol was dissolved in the same solvent as TFH and magnetic fluid was added to the lipid solution.…”

Section: Methodsmentioning

confidence: 99%

Preparation and characterization of manganese ferrite-based magnetic liposomes for hyperthermia treatment of cancer

Pradhan¹,

Giri

Banerjee³

et al. 2007

Journal of Magnetism and Magnetic Materials

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“…Regardless, we have been able to prepare liposome/DNA complexes using cationic lipid/EPC mixtures that exhibit the same size distribution as cationic lipid/DOPE mixtures through the use of ultrasonic cavitation. We suggest that other methods of size reduction, which employ mechanical force based on cavitation, such as homogenization, 53 high-pressure extrusion, 54 or microfluidization, [55][56][57] may also be able to produce particles of small, uniform diameter from these complexes.…”

Section: Discussionmentioning

confidence: 98%

Plasmid DNA is Protected against Ultrasonic Cavitation-Induced Damage when Complexed to Cationic Liposomes

Wasan¹,

Reimer²,

Bally³

1996

Journal of Pharmaceutical Sciences

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Cationic liposomes bound to plasmid DNA are currently used for in vitro and in vivo gene therapy applications, but such complexes readily form large, heterogeneous aggregates that are not appropriate for pharmaceutical development. More importantly, size heterogeneity makes studies focused on optimizing gene transfer to cells difficult to conduct or understand. For this reason we have evaluated the effect of microprobe sonication on these complexes in an effort to achieve process-controlled size homogeneity. Complexes were prepared using a 7.2 kb reporter plasmid and the following liposomal lipid combinations: DDAB/DOPE (50:50 mol %), DDAB/DOPE/PEG-PE (50:45:5 mol %), DDAB/EPC (50:50 mol %), DDAB/EPC/PEG-PE (50:45:5, 50:40:10, 50:35:15 mol %), DODAC/DOPE (50:50 mol %), and DODAC/EPC (50:50 mol %) (DDAB, dimethyldioctadecylammonium bromide; DOPE, dioleoylphosphatidylethanolamine; PEG-PE, monomethoxypolyethylene glycol2000 succinate- distearoylphosphatidylethanolamine; EPC, egg phosphatidylcholine; DODAC, dioleoyldimethylammonium chloride). The influence of complex composition and lipid:DNA ratio was evaluated. Particle size was determined before and after complexation and again after sonication using the quasi-elastic light scattering technique. DNA integrity was assessed via agarose gel electrophoresis. Finally, gene transfection was evaluated using CHO cells that were transfected in vitro with sonicated and unsonicated complexes. It is established in this study that size reduction can occur, but this is dependent on cationic and neutral lipid composition and, in some cases, lipid:DNA ratio. Surprisingly, the process of sonication leaves a significant percentage of the plasmid DNA intact and capable of in vitro transfection. This study shows that plasmid DNA can be protected from damage due to sonication by liposome complex formation. This may indicate that more common pharmaceutical methods for size reduction which subject particles to mechanical stress may be applicable in preparation of liposome/DNA formulations for in vivo application.

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“…al. [10][11][12]. To maximize oxygen-carrying capacity high concentrations of hemoglobin solution up to 3Sg"/o (i.e.…”

Section: Hemoglobin Solution Preparationmentioning

confidence: 99%

Liposome Microencapsulations Without Using Any Organic Solvent

Zheng

Alkan-Önyüksel

Beissinger

et al. 1999

Journal of Dispersion Science and Technology

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Liposomes are microscopic vesicles composed of phospholipid bilayers surrounding discrete aqueous compartment Eventhough phospholipid bilayers are formed spontaneously in water, their conventional preparation methods involve either organic solvents or detergent molecules, Organic solvents may create a serious safety problem during liposome manufacturing especially on a large scale and can cause denaturation of proteins and affect the membrane properties. Liposomes are very promising carriers for protein drugs which have aroused great interests in the field of pharmaceutics in these recent years,The objective of this study was to develop a new dispersion process to prepare Iiposomes without the usage of organic solvents and detergents, The approach involved the usage of a microfludizer for the extrusion and homogenization of aqueous dispersion of phospholipids, and a modified evaporator in order to increase lipid thin film surface area, The properties of the liposomes prepared by the novel method were superior to the liposomes prepared by conventional methods,

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Microencapsulation of hemoglobin in liposomes using a double emulsion, film dehydration/rehydration approach

Cited by 24 publications

References 32 publications

Preparation and characterization of manganese ferrite-based magnetic liposomes for hyperthermia treatment of cancer

Preparation and characterization of manganese ferrite-based magnetic liposomes for hyperthermia treatment of cancer

Plasmid DNA is Protected against Ultrasonic Cavitation-Induced Damage when Complexed to Cationic Liposomes

Liposome Microencapsulations Without Using Any Organic Solvent

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