Microencapsulation of Insulin into Biodegradable Microcapsules by the High Voltage Field Method

Abstract

To produce and evaluate sustained microcapsules of insulin made by a novel encapsulation process. The insulin was encapsulated into microcapsules of poly(D, L-lactide co-glycolide) (PLGA) by the high voltage field method. The preparation process was cryogenic, non-aqueous, and did not utilize emulsification. When the ratio of insulin/PLGA was 1:9, the median particle size of insulin microcapsules was less than 200μm, the drug loaded was 8.82%, and the efficiency of encapsulation was 83.2%. Drug release continued over 300h. In conclusion, the process was a novel way to produce insulin microcapsules, and the basic characteristics were satisfactory.

Novel noninvasive techniques in management of diabetes

“…Over 90% of the entrapped insulin released after 3 days. [82,83] Zhou et al prepared sustained microcapsules of insulin made by a novel high-voltage field method; showed drug release continued over 300 h. [84] poly (alkylcyanoacrylate) nanocapsules prepared by Interfacial polymerization of spontaneously forming water-in-oil microemulsion represents an efficient way to load proteins and peptide drugs. [85] In similar approach Graf et al developed poly (alkylcyanoacrylate) nanoparticles prepared from microemulsion, showed that hypoglycemic effect over controls up to 36 h. [86] Research in this area leads to the development of CS-MPs by membrane emulsification technique and a step-wise crosslinking method, provided the microspheres with higher chemical stability of insulin (>95%), lower burst release and steady release behavior.…”