Microencapsulation of isolated pituitary cells by polyacrylamide microlatex coagulation on agarose beads

Dupuy, B.; Cadic, Ch.; Gin, H.; Baquey, C.; Dufy, B; Ducassou, D.

doi:10.1016/0142-9612(91)90148-4

Cited by 20 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the potential effects of its further application to a useful extracorporeal circuit in conjunction with hemodialysis filters remain a major concern. In fact, recent years have witnessed increasing comparable researches related to the therapeutic use of polyacrylamide in literature (20–23). Of note, Bosch et al developed a DALI system based on modified polyacrylate ligands immobilized on a polyacrylamide matrix that enables direct adsorption of lipoproteins from whole blood with lack of detrimental effects on blood cells and most plasma parameters.…”

Section: Discussionmentioning

confidence: 99%

A Serial Copolymerization Procedure for Manufacturing Immunoadsorption Walls as a Potential Unit in Conjunction With Hemodialysis Filters

Yang

2006

Artificial Organs

View full text Add to dashboard Cite

A functional immunoadsorption wall for removal of beta-2-microglobulin has been made by partially incomplete two-stage copolymerization of acrylamide with immunoadsorbent. However, a substantial amount of immunoadsorbent needs to be flushed away after the copolymerization process. Thus, to enhance the utilization efficiency of immunoadsorbent, the flushed-away immunoadsorbent was further recovered, and the copolymerization was conducted in series to produce three consecutive immunoadsorption walls in this study. Preliminary removal tests show that similar removal patterns were obtained for these immunoadsorption walls. Although it is not timely to conclude that a clinically applicable immunoadsorption wall has taken shape, the development of a partially incomplete two-stage polymerization method and its associated techniques indeed provide a good basis for large-scale manufacturing immunoadsorption walls.

show abstract

Section: Discussionmentioning

confidence: 99%

A Serial Copolymerization Procedure for Manufacturing Immunoadsorption Walls as a Potential Unit in Conjunction With Hemodialysis Filters

Yang

2006

Artificial Organs

View full text Add to dashboard Cite

show abstract

“…Dupuy et al 19 reported enclosure of cells into a polyacrylamide microcapsule by radical polymerization and claimed its superior biocompatibility. However, it was necessary to entrap the cells into an agarose microcapsule prior to the polymerization of the acrylamide to avoid direct contact of the cells with the cytotoxic acrylamide and initiator of radical polymerization.…”

Section: Discussionmentioning

confidence: 99%

Entrapment of islets into reversible disulfide hydrogels

Hisano

Morikawa

Iwata

et al. 1998

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Currently, there are three types of devices for a bioartificial pancreas; microencapsulation, an extravascular diffusion chamber, and an intravascular diffusion chamber. The purpose of the present study was to provide a new extracellular matrix hydrogel for the devices of extra- and intravascular diffusion chamber types. As the sol-gel transition of this hydrogel is reversible, refilling of islets in vivo will be possible without a severe traumatic procedure. The hydrogel was produced from a polyacrylamide derivative carrying thiol groups synthesized by radical copolymerization of acrylamide and N,N'-bis-acrylcystamine, followed by reduction of the disulfide bonds in the copolymer. This water-soluble copolymer was used to entrap hamster islets by re-formation of disulfide bonds on the copolymer to produce a hydrogel. The formed hydrogel was easily reliquefied by reduction of the disulfide crosslinks to thiols. Insulin release from the islet-entrapped hydrogel continued for more than 1 month when examined in vitro. A static glucose stimulation test for the entrapped islets exhibited an increased insulin release.

show abstract

“…The authors demonstrated a lack of cytotoxicity for encapsulated islets of Langerhans by measuring the insulin secretion at the end of the different steps. The second embedding procedure has been adapted for the encapsulation of pituitary cells (Dupuy et al, 1991). Part of the acrylamide monomers was prepolymerised inside a microemulsion, resulting in micro-latex beads of homogenous size.…”

Section: Agarose-acrylamidementioning

confidence: 99%

Gel Entrapment and Micro-Encapsulation: Methods, Applications and Engineering Principles

Willaert¹,

Baron²

1996

Reviews in Chemical Engineering

113

View full text Add to dashboard Cite

Microencapsulation of isolated pituitary cells by polyacrylamide microlatex coagulation on agarose beads

Cited by 20 publications

References 16 publications

A Serial Copolymerization Procedure for Manufacturing Immunoadsorption Walls as a Potential Unit in Conjunction With Hemodialysis Filters

A Serial Copolymerization Procedure for Manufacturing Immunoadsorption Walls as a Potential Unit in Conjunction With Hemodialysis Filters

Entrapment of islets into reversible disulfide hydrogels

Gel Entrapment and Micro-Encapsulation: Methods, Applications and Engineering Principles

Contact Info

Product

Resources

About