Microencapsule technique protects hepatocytes from cryoinjury

Kusano, Tomokazu; Aoki, Takeshi; Yasuda, Daisuke; Matsumoto, Shuichiro; Jin, Zhenghao; Nishino, Nobukazu; Hayashi, Ken; Odaira, Masanori; Yamada, Kunio; Koizumi, Toru; Izumida, Yoshihiko; Mitamura, Keiji; Enami, Yuta; Niiya, Takashi; Murai, Noriyuki; Kato, Hirohisa; Shimizu, Yoshinori; Kou, Keitatsu; Furukawa, Yoshinori; Matsusita, Michiaki; Todo, Satoru; Shioda, Seiji; Kusano, Mitsuo

doi:10.1111/j.1872-034x.2007.00311.x

Cited by 19 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…18,19,20 However, our current work provided no evidence for this. Indeed, ELS stored at -80°C showed progressive injury over the 12 months, which argues against any evidence for a major physical protection by encapsulation during storage at temperatures such as -80°C.…”

mentioning

confidence: 58%

Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Massie

Selden²,

Hodgson³

et al. 2013

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

mentioning

confidence: 58%

Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Massie

Selden²,

Hodgson³

et al. 2013

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

“…[7] reported the successful transplantation of cryopreserved tracheal allograft in a rabbit model. Other authors have reported the successful cryopreservation of hepatocytes [8,9] and whole murine and porcine livers [10]. …”

Section: Introductionmentioning

confidence: 99%

Cryopreservation and replantation of amputated rat hind limbs

Wang

Duan

et al. 2014

Eur J Med Res

View full text Add to dashboard Cite

BackgroundIn spite of the relatively high success rate of limb replantation, many patients cannot undergo replantation surgery because the preservation time of an amputated limb is only about six hours. In addition, although allotransplantation of composite tissues is being performed more commonly with increasingly greater success rates, the shortage of donors limits the number of patients that can be treated. So the purpose of this study is to examine the feasibility of cryopreservation and replantation of limbs in a rat model.MethodsTwelve five-month-old Sprague-Dawley rats were divided evenly into group A (above-knee amputation) and group B (Syme’s amputation). One hind limb was amputated from each rat. The limbs were irrigated with cryoprotectant, cooled in a controlled manner to -140°C, and placed in liquid nitrogen. Thawing and replantation were performed 14 days later.ResultsIn group A, the limbs became swollen after restoration of blood flow resulting in blood vessel compression and all replantations failed. In group B, restoration of blood flow was noted in all limbs after replantation. In one case, the rat chewed the replanted limb and replantation failed. The other five rats were followed for three months with no abnormalities noted in the replanted limbs.ConclusionsLimbs with a minimal amount of muscle tissue can be successfully cryopreserved and replanted.

show abstract

“…Cryopreservation of cells microencapsulated in alginate has previously shown success in maintaining cell viability, stem cell characteristics, and cellular recovery [42–45]. Cryopreservation allows for thorough testing of the encapsulated MSCs with the ability to thaw samples for validating cell viability, therapeutic protein concentration, sterility, and microbead integrity for ensuring that the highest-quality production has been performed.…”

Section: Introductionmentioning

confidence: 99%

Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs

Mumaw

Jordan

Sonnet

et al. 2012

International Journal of Biomaterials

View full text Add to dashboard Cite

Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce rapid bone formation. However, methods to make these treatments available for clinical applications are presently lacking. In this study we used mesenchymal stem cells (MSCs) due to their ease of harvest, replication potential, and immunomodulatory capabilities. MSCs were from sheep and pig due to their appeal as large animal models for bone nonunion. We demonstrated that cryopreservation of these microencapsulated MSCs did not affect their cell viability, adenoviral BMP2 production, or ability to initiate bone formation. Additionally, microspheres showed no appreciable damage from cryopreservation when examined with light and electron microscopy. These results validate the use of cryopreservation in preserving the viability and functionality of PEG-encapsulated BMP2-transduced MSCs.

show abstract

Microencapsule technique protects hepatocytes from cryoinjury

Abstract: Our microencapsulation technique protects hepatocytes from cryoinjury. This novel technique could be utilized by hepatocyte banks.

Cited by 19 publications

References 26 publications

Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Storage Temperatures for Cold-Chain Delivery in Cell Therapy: A Study of Alginate-Encapsulated Liver Cell Spheroids Stored at −80°C or −170°C for Up to 1 Year

Cryopreservation and replantation of amputated rat hind limbs

Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs

Contact Info

Product

Resources

About