Microenvironment and tumor progression of melanoma: New therapeutic prospectives

Botti, Gerardo; Cerrone, Margherita; Scognamiglio, Giosuè; Anniciello, Annamaria; Ascierto, Paolo A.; Cantile, Monica

doi:10.3109/1547691x.2012.723767

Cited by 43 publications

(25 citation statements)

References 200 publications

(183 reference statements)

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“…Melanomas modify their microenvironment through secretion of specific extracellular matrix proteins and extracellular matrix modifying proteases to promote tumor growth and metastasis [48]. Alterations in extracellular matrix remodelling have been shown to be an important event during EMT [46].…”

Section: Discussionmentioning

confidence: 99%

Identifying and targeting determinants of melanoma cellular invasion

et al. 2016

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Epithelial-to-mesenchymal transition is a critical process that increases the malignant potential of melanoma by facilitating invasion and dissemination of tumor cells. This study identified genes involved in the regulation of cellular invasion and evaluated whether they can be targeted to inhibit melanoma invasion. We identified Peroxidasin (PXDN), Netrin 4 (NTN4) and GLIS Family Zinc Finger 3 (GLIS3) genes consistently elevated in invasive mesenchymal-like melanoma cells. These genes and proteins were highly expressed in metastatic melanoma tumors, and gene silencing led to reduced melanoma invasion in vitro. Furthermore, migration of PXDN, NTN4 or GLIS3 siRNA transfected melanoma cells was inhibited following transplantation into the embryonic chicken neural tube compared to control siRNA transfected melanoma cells. Our study suggests that PXDN, NTN4 and GLIS3 play a functional role in promoting melanoma cellular invasion, and therapeutic approaches directed toward inhibiting the action of these proteins may reduce the incidence or progression of metastasis in melanoma patients.

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Section: Discussionmentioning

confidence: 99%

Identifying and targeting determinants of melanoma cellular invasion

et al. 2016

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“…Also in the pathogenesis of other neoplasms, such as melanoma, neuroendocrine tumors, and multiple myeloma, the microenvironment plays an important role in the evolution of tumor burden and the acquisition of a metastatic phenotype [106][107][108]. Several studies have demonstrated that angiogenesis is associated with poor prognosis in patients with metastatic melanoma [109,110], and microtubule stabilizers and cyclooxygenase (COX)-2 inhibitors, alone or in combination, have shown inhibitory effects on endothelial cells and tumor angiogenesis [111].…”

Section: Melanoma Neuroendocrine Tumors Multiple Myelomamentioning

confidence: 98%

Metronomic chemotherapy from rationale to clinical studies: A dream or reality?

Gaspar

Silvestris

Licchetta

et al. 2015

Critical Reviews in Oncology/Hematology

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“…Our results are in line with previous comparative studies of in vivo situations as well as in vitro 2D cultures (27) and emphasize the importance of the tumor microenvironment. The efficacy of chemotherapeutics and novel therapeutic approaches considerably depends on the extracellular matrix components, their organization, adhesion molecules as well as cell-cell contacts (28,29). Therefore, we focused on the evaluation of optimized size for TMF.…”

Section: Discussionmentioning

confidence: 99%

Direct Chemosensitivity Monitoring Ex Vivo on Undissociated Melanoma Tumor Tissue by Impedance Spectroscopy

Jahnke¹,

Poenick²,

Maschke

et al. 2014

Cancer Research

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Stage III/IV melanoma remains incurable in most cases due to chemotherapeutic resistance. Thus, predicting and monitoring chemotherapeutic responses in this setting offer great interest. To overcome limitations of existing assays in evaluating the chemosensitivity of dissociated tumor cells, we developed a label-free monitoring system to directly analyze the chemosensitivity of undissociated tumor tissue. Using a preparation of tumor micro-fragments (TMF) established from melanoma biopsies, we characterized the tissue organization and biomarker expression by immunocytochemistry. Robust generation of TMF was established successfully and demonstrated on a broad range of primary melanoma tumors and tumor metastases. Organization and biomarker expression within the TMF were highly comparable with tumor tissue, in contrast to dissociated, cultivated tumor cells. Using isolated TMF, sensitivity to six clinically relevant chemotherapeutic drugs (dacarbazine, doxorubicin, paclitaxel, cisplatin, gemcitabine, and treosulfan) was determined by impedance spectroscopy in combination with a unique microcavity array technology we developed. In parallel, comparative analyses were performed on monolayer tumor cell cultures. Lastly, we determined the efficacy of chemotherapeutic agents on TMF by impedance spectroscopy to obtain individual chemosensitivity patterns. Our results demonstrated nonpredictable differences in the reaction of tumor cells to chemotherapy in TMF by comparison with dissociated, cultivated tumor cells. Our direct impedimetric analysis of melanoma biopsies offers a direct ex vivo system to more reliably predict patient-specific chemosensitivity patterns and to monitor antitumor efficacy. Cancer Res; 74(22); 6408-18. Ó2014 AACR.

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Microenvironment and tumor progression of melanoma: New therapeutic prospectives

Cited by 43 publications

References 200 publications

Identifying and targeting determinants of melanoma cellular invasion

Identifying and targeting determinants of melanoma cellular invasion

Metronomic chemotherapy from rationale to clinical studies: A dream or reality?

Direct Chemosensitivity Monitoring Ex Vivo on Undissociated Melanoma Tumor Tissue by Impedance Spectroscopy

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