Microenvironmental factors regulate Ly-6 A/E expression on Py V-transformed BALB/c 3T3 cells

Eshel, Rinat; Firon, Michal; Katz, Ben‐Zion; Sagi‐Assif, Orit; Aviram, Hadar; Witz, I P

doi:10.1016/0165-2478(94)00216-e

Cited by 8 publications

(8 citation statements)

References 9 publications

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“…In addition, Ly-6 expression may contribute to tumorigenicity (27) and metastasis (28). Previous studies have suggested the presence of Ly-6A.2 ligand(s) on lymphoid cells (13).…”

Section: Discussionmentioning

confidence: 99%

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A Monoclonal Antibody Against the 66-kDa Protein Expressed in Mouse Spleen and Thymus Inhibits Ly-6A.2-Dependent Cell-Cell Adhesion

English

Kosoy

Pawlinski

et al. 2000

The Journal of Immunology

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The Ly-6 locus encodes several cell surface proteins of 10–12 kDa. Some members of this multigene family may function in cell signaling and/or cell adhesion processes. T lymphocytes overexpressing Ly-6A.2 (one member of the Ly-6 gene family) protein homotypically aggregate when cultured in vitro. Further analysis of this homotypic aggregation suggests that Ly-6A.2 participates in cell-cell adhesion. These observations indicated the presence of a Ly-6 ligand(s) on the surface of lymphoid cells. In this study we report generation of a hamster mAb, 9AB2, that blocks Ly-6A.2-dependent cell-cell adhesion. The 9AB2 Ab recognizes a 66-kDa glycoprotein with unique tissue expression. The 9AB2 mAb does not bind Ly-6A.2, but coimmunoprecipitates Ly-6A.2 molecule. Moreover, 9AB2 Ag-expressing thymocytes specifically bind to Chinese hamster ovary cells overexpressing Ly-6A.2 protein, and this binding is specifically blocked by 9AB2 and anti-Ly-6A.2 Abs. These results suggest that the 66-kDa protein recognized by 9AB2 mAb is the putative ligand for Ly-6A.2.

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“…In addition, Ly-6 expression may contribute to tumorigenicity (27) and metastasis (28). Previous studies have suggested the presence of Ly-6A.2 ligand(s) on lymphoid cells (13).…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have suggested a role for Ly-6A/E expression in tumorigenicity (27) and metastases (28). One human homologue of the mouse Ly-6 gene, prostate stem cell Ag, is known to be prostate specific and therefore can be a potential target for diagnosis as well as therapy (38).…”

Section: Cd8mentioning

confidence: 99%

A Monoclonal Antibody Against the 66-kDa Protein Expressed in Mouse Spleen and Thymus Inhibits Ly-6A.2-Dependent Cell-Cell Adhesion

English

Kosoy

Pawlinski

et al. 2000

The Journal of Immunology

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“…Membrane Ly-6 levels on tumor cells were upregulated by interferon ␣ (IFN␣) and by coating such cells with monoclonal antibodies directed against Ly-6E.1 [Eshel et al, 1995]. Ly-6E.1 levels were slightly downregulated by tumor necrosis factor-␣ (TNF␣) and drastically downregulated by anti Fas antibodies [SagiAssif et al, 1996].…”

Section: Microenvironment-regulated Genes and The Involvement Of Murimentioning

confidence: 99%

“…The work in our laboratory focuses on tumor-CMF interactions trying to identify genes whose expression is altered (induced/upregulated or silenced/downregulated) by CMF-mediated signaling as low malignancy tumor cells progress to a high malignancy phenotype [Zusman et al, 1996a;Katz et al, 1994Katz et al, , 1994Katz et al, -1995Witz et al, 1996;Halachmi and Witz, 1989;Ben-Baruch et al, 1992;Ran et al, 1991]. The approach undertaken has been to screen for microenvironmentregulated gene-products differentially expressed by tumor cells expressing either a high or a low malignancy phenotype [Katz et al, 1994;Witz et al, 1996;Halachmi and Witz, 1989].…”

Section: Microenvironment-regulated Genes and The Involvement Of Murimentioning

confidence: 99%

Differential expression of genes by tumor cells of a low or a high malignancy phenotype: The case of murine and human Ly-6 proteins

Witz¹

2000

J. Cell. Biochem.

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Progression of tumor cells toward a high malignancy phenotype and metastasis is a multi-event cascade involving inter alia alterations in the expression of various genes. The focus of our laboratory is on genes whose altered expression may lead, directly or indirectly, to an increased malignancy phenotype. The identification of such genes and the evaluation of the consequences of their altered expression is essential for attempts to halt tumor progression and prevent metastasis formation. Published work originating in our laboratory showed that members of the murine Ly-6 supergene family are involved in the progression of certain mouse tumors. The expression level of several members of this family was higher on highly malignant cells than on tumor cells expressing a lower malignancy phenotype. Sorting by flow cytometry of tumor cells to subpopulations expressing either high or low levels of Ly-6E.1 yielded correspondingly cells expressing a high or a low malignancy phenotype. The high malignancy, high Ly-6E.1-expressing cells also expressed high levels of the receptor for urokinase plasminogen activator (uPAR), whereas low malignancy, low Ly-6E.1-expressing cells also expressed low levels of uPAR. Transfection studies indicated that uPAR was causally involved in conferring a high malignancy phenotype upon tumor cells expressing high levels of Ly-6E.1. E48 is a human homologue of the murine ThB protein, a member of the Ly-6 supergene family (but distinct from the Ly-6E.1 protein mentioned above) and expressed on head and neck squamous carcinoma cells. Experiments currently in progress are aimed to find out whether E48 is involved in the progression of such cancer cells. Using the differential display technology, it was shown that ligation of E48 on tumor cells by the corresponding antibodies (serving as a surrogate for an as yet unidentified E48 ligand) upregulates an enzyme (FX) involved in the biosynthesis of GDP-L-fucose. Fucose is an essential component of certain selectin ligands.

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Section: Microenvironment-regulated Genes and The Involvement Of Murimentioning

confidence: 99%

Differential expression of genes by tumor cells of a low or a high malignancy phenotype: The case of murine and human Ly-6 proteins

Witz¹

2000

J. Cell. Biochem.

View full text Add to dashboard Cite

Progression of tumor cells toward a high malignancy phenotype and metastasis is a multi‐event cascade involving inter alia alterations in the expression of various genes. The focus of our laboratory is on genes whose altered expression may lead, directly or indirectly, to an increased malignancy phenotype. The identification of such genes and the evaluation of the consequences of their altered expression is essential for attempts to halt tumor progression and prevent metastasis formation. Published work originating in our laboratory showed that members of the murine Ly‐6 supergene family are involved in the progression of certain mouse tumors. The expression level of several members of this family was higher on highly malignant cells than on tumor cells expressing a lower malignancy phenotype. Sorting by flow cytometry of tumor cells to subpopulations expressing either high or low levels of Ly‐6E.1 yielded correspondingly cells expressing a high or a low malignancy phenotype. The high malignancy, high Ly‐6E.1‐expressing cells also expressed high levels of the receptor for urokinase plasminogen activator (uPAR), whereas low malignancy, low Ly‐6E.1‐expressing cells also expressed low levels of uPAR. Transfection studies indicated that uPAR was causally involved in conferring a high malignancy phenotype upon tumor cells expressing high levels of Ly‐6E.1. E48 is a human homologue of the murine ThB protein, a member of the Ly‐6 supergene family (but distinct from the Ly‐6E.1 protein mentioned above) and expressed on head and neck squamous carcinoma cells. Experiments currently in progress are aimed to find out whether E48 is involved in the progression of such cancer cells. Using the differential display technology, it was shown that ligation of E48 on tumor cells by the corresponding antibodies (serving as a surrogate for an as yet unidentified E48 ligand) upregulates an enzyme (FX) involved in the biosynthesis of GDP‐L‐fucose. Fucose is an essential component of certain selectin ligands. J. Cell. Biochem. Suppl. 34:61–66, 2000. © 2000 Wiley‐Liss, Inc.

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Microenvironmental factors regulate Ly-6 A/E expression on Py V-transformed BALB/c 3T3 cells

Cited by 8 publications

References 9 publications

A Monoclonal Antibody Against the 66-kDa Protein Expressed in Mouse Spleen and Thymus Inhibits Ly-6A.2-Dependent Cell-Cell Adhesion

A Monoclonal Antibody Against the 66-kDa Protein Expressed in Mouse Spleen and Thymus Inhibits Ly-6A.2-Dependent Cell-Cell Adhesion

Differential expression of genes by tumor cells of a low or a high malignancy phenotype: The case of murine and human Ly-6 proteins

Differential expression of genes by tumor cells of a low or a high malignancy phenotype: The case of murine and human Ly-6 proteins

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