Microextraction by packed sorbent (MEPS): A tutorial

Abdel‐Rehim, Mohamed

doi:10.1016/j.aca.2011.05.037

Cited by 235 publications

(86 citation statements)

References 40 publications

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“…10 Protein precipitation can result in a high matrix background and significant signal suppression is often observed. LLE and SPE methods require large quantities of samples and solvents or solvent mixtures.…”

Section: Introductionmentioning

confidence: 99%

“…11,12 However, in SPME the sampling fiber is quite sensitive to the nature of the sample matrices, such as urine, plasma or blood. 13 Recently, a new method of sample preparation, termed as microextraction in a packed syringe (MEPS), 10,[14][15][16][17][18] has been introduced for environmental studies as well as pharmacology, toxicology and forensic sciences, and has attracted a considerable attention. The MEPS technique, miniaturizes the SPE so that it can work with small sample volumes in the range of 10 to 250 μL.…”

Section: Introductionmentioning

confidence: 99%

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Microextraction in a Packed Syringe for the Analysis of Olive Biophenols in Rat Plasma Using CMK-3 Nanoporous Sorbent

et al. 2013

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A carbon-based nanoporous sorbent was first used for microextraction in a packed syringe (MEPS) before HPLC/UV analysis of some biophenols in rat plasma. A laboratory-made programmable apparatus was designed and used for automation of the extraction procedure. The MEPS syringe was packed with 2 mg of CMK-3 sorbent, between the barrel and the injection needle, and mounted on an apparatus for programming of the conditioning, sampling, washing, elution and cleaning steps. All steps of the microextraction procedure were carefully optimized on the system. For optimization of important factors, such as the number of adsorption and elution cycles, elution volume and pH, a multivariate central composite design method was used. The highest recoveries were obtained for 24 and 10 times of adsorption and elution cycles, respectively, using 100 μL of acetonitrile as the eluent and a sample pH of 2. Good results were obtained in terms of the precision (RSD 1.6, 2.5 and 2.3%) and detection limit (0.7, 4.7 and 0.25 μM) for caffeic acid, tyrsol and oleuropein, respectively. The method was simple, efficient and appropriate for sample clean up before analysis by HPLC, and was successfully applied to the determination of biophenols in the plasma of several rats that received an olive leaves extract either by a gavage or an intraperitoneal injection method. A positive correlation was found between the amount of olive extract's feeding of the rats and the level of their plasma biophenols.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microextraction in a Packed Syringe for the Analysis of Olive Biophenols in Rat Plasma Using CMK-3 Nanoporous Sorbent

et al. 2013

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“…The adsorbed analyte is subsequently eluted by a suitable organic solvent, such as dichloromethane (DCM), n-hexane, and methanol. 15,16 In this technique, the amount of sorbent, volume of the sample solution, the volume of the desorption solvent are miniaturized. MEPS differs from commercial solid-phase extraction in that a little amount of the sorbent is used in the syringe, and thus it allows sparing the reagent and the high-cost sorbent.…”

Section: Introductionmentioning

confidence: 99%

Determination of Three Organochlorine Pesticides in Aqueous Samples by Solid-Phase Extraction Based on Natural Nano Diatomite in Packed Syringe Coupled to Gas Chromatography-Mass Spectrometry

et al. 2017

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“…1 mL of organic solvents. The most frequent uses of the MEPS technique cover the analyses of drugs and their metabolites in biological samples [16][17][18] but also the analyses of fresh water contamination [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…Several new extraction-separation techniques suitable for the use with low amounts of the sample were designed recently. Among them, microextraction by packed sorbent (MEPS™; SGE Analytical Science, Australia), which is a small-scale format of a classic solid-phase extraction, offers both polar and hydrophobic sorbents and is well suited to be used with chromatographic systems [16][17][18]. Reusable sorbent bed is integrated into a syringe, which may be used either manually or automatically to process the small volume (10 μL or more) of the liquid sample.…”

Section: Introductionmentioning

confidence: 99%

Fractionation of Cuticular Waxes from the Leaves of Solanaceae Plant Species Using Microextraction by Packed Sorbent

Haliński

Stepnowski

2015

Acta Chromatographica

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Summary.The microextraction by packed sorbent (MEPS) was applied to a fractionation of cuticular wax extracts from several solanaceous plant species. A procedure developed requires only 0.6 mL of organic solvents and may be completed in less than 10 min. Hydrocarbons, which are frequently used in chemotaxonomy of Solanaceae, were almost exclusively eluted in one fraction. The amounts of most commonly detected polar wax components (alcohols, sterols, triterpenes) in the same fraction were reduced to ca. 34-46% of the total amounts in the whole extract. Despite the contamination of the hydrocarbon fraction with other wax components, the results obtained using MEPS and standard column chromatography on silica gel were similar when compared using cluster analysis based on the hydrocarbon profiles. However, the method was far less successful in removing the sucrose esters from extracts of Nicotiana rustica leaves. Thus, MEPS fractionation of plant cuticular waxes may be a fast and reliable alternative for the standard liquid chromatography techniques as long as no sucrose esters are present in the extract.

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Microextraction by packed sorbent (MEPS): A tutorial

Cited by 235 publications

References 40 publications

Microextraction in a Packed Syringe for the Analysis of Olive Biophenols in Rat Plasma Using CMK-3 Nanoporous Sorbent

Microextraction in a Packed Syringe for the Analysis of Olive Biophenols in Rat Plasma Using CMK-3 Nanoporous Sorbent

Determination of Three Organochlorine Pesticides in Aqueous Samples by Solid-Phase Extraction Based on Natural Nano Diatomite in Packed Syringe Coupled to Gas Chromatography-Mass Spectrometry

Fractionation of Cuticular Waxes from the Leaves of Solanaceae Plant Species Using Microextraction by Packed Sorbent

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