Microfabrication of poly (glycerol–sebacate) for contact guidance applications

Bettinger, Christopher J.; Orrick, Brian K.; Misra, Asish C.; Langer, Robert; Borenstein, Jeffrey T.

doi:10.1016/j.biomaterials.2005.11.029

Cited by 215 publications

(171 citation statements)

References 30 publications

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“…The 600 nm width for ridge and groove features was chosen to promote optimal contact guidance effects in EPCs by minimizing feature masking from the collagen coating and maximizing cell alignment through sub-micrometer features. [10,13,14] The EPCs seeded as individual cells on nanotopographic substrates and responded to linear nanotopography by alteration in morphology as observed by increased alignment and elongation ( Fig. 1).…”

mentioning

confidence: 94%

“…[7][8][9] We hypothesized that physical features on nanofabricated substrates could promote the organization of endothelial cell lineages into well-defined vascular structures in vitro by inducing the contact guidance phenomenon, which is known to affect the morphology of endothelial cells. [10][11][12] We found that endothelial progenitor cells (EPCs) responded to ridge-groove grating of 1200 nm in period and 600 nm in depth through alignment, elongation, reduced proliferation, and enhanced migration. Although endothelial-specific markers were not significantly altered, EPCs cultured on substrate nanotopography formed supercellular band structures after 6 d. Furthermore, an in vitro Matrigel assay led to enhanced capillary tube formation and organization.…”

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confidence: 98%

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Enhancement of In Vitro Capillary Tube Formation by Substrate Nanotopography

et al. 2007

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confidence: 94%

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confidence: 98%

Enhancement of In Vitro Capillary Tube Formation by Substrate Nanotopography

et al. 2007

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“…Prominent examples of bioabsorbable elastomers include polyurethane-ureas, poly(glycerol-co-sebacate) (PGS), poly(1,8-octanediol-cocitrate), polyurethanes, and their derivatives (Wang et al, (Minev et al, 2015) 2002; Yang et al, 2004;Bettinger et al, 2008;Bettinger, 2011). Like silicones, bioabsorbable elastomers are extensible, can be synthesized in large quantities, processed into thin films, and are compatible with soft lithography and microfabrication techniques (Bettinger et al, 2006a;Bettinger et al, 2006b) including micromolding (Bettinger et al, 2006a), photocrosslinking (Nijst et al, 2007), and 3D-printing (Hung et al, 2014). Unlike silicones, bioabsorbable elastomers are often hygroscopic and susceptible to degradation through hydrolysis and enzymatic activity (Wang et al, 2003;Bettinger et al, 2009).…”

Section: Bioabsorbable Substrates and Components For Stretchable And mentioning

confidence: 99%

Recent advances in materials and flexible electronics for peripheral nerve interfaces

Bettinger

2018

Bioelectron Med

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Peripheral nerve interfaces are a central technology in advancing bioelectronic medicines because these medical devices can record and modulate the activity of nerves that innervate visceral organs. Peripheral nerve interfaces that use electrical signals for recording or stimulation have advanced our collective understanding of the peripheral nervous system. Furthermore, devices such as cuff electrodes and multielectrode arrays of various form factors have been implanted in the peripheral nervous system of humans in several therapeutic contexts. Substantive advances have been made using devices composed of off-the-shelf commodity materials. However, there is also a demand for improved device performance including extended chronic reliability, enhanced biocompatibility, and increased bandwidth for recording and stimulation. These aspirational goals manifest as much needed improvements in device performance including: increasing mechanical compliance (reducing Young's modulus and increasing extensibility); improving the barrier properties of encapsulation materials; reducing impedance and increasing the charge injection capacity of electrode materials; and increasing the spatial resolution of multielectrode arrays. These proposed improvements require new materials and novel microfabrication strategies. This mini-review highlights selected recent advances in flexible electronics for peripheral nerve interfaces. The foci of this mini-review include novel materials for flexible and stretchable substrates, non-conventional microfabrication techniques, strategies for improved device packaging, and materials to improve signal transduction across the tissue-electrode interface. Taken together, this article highlights challenges and opportunities in materials science and processing to improve the performance of peripheral nerve interfaces and advance bioelectronic medicine.

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“…Cellular responses to synthetic micro-and nanofabricated substrates [9] via contact guidance [10] has been observed in a variety of mature cell types [11] including, but not limited to epithelial cells [12][13][14], fibroblasts [15][16][17][18], oligodendrocytes [19], astrocytes [19], and endothelial cells [20]. In addition to changes in gross morphology and migration, contact guidance induced up regulation of fibronectin mRNA in human fibroblasts [21], increased adhesion of epithelial cells [13], and increased mineralization and alkaline phosphatase activity in rat bone marrow cells [22].…”

Section: Introductionmentioning

confidence: 99%

The effect of actin disrupting agents on contact guidance of human embryonic stem cells

et al. 2007

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Mammalian cells respond to their substrates by complex changes in gene expression profiles, morphology, proliferation and migration. We report that substrate nanotopography alters morpohology and proliferation of human embryonic stem cells (hESCs). Fibronectin-coated poly(dimethyl siloxane) substrates with line-grating (600 nm ridges with 600 nm spacing and 600 +/− 150 nm feature height) induced hESC alignment and elongation, mediated the organization of cytoskeletal components including actin, vimentin, and α-tubulin, and reduced proliferation. Spatial polarization of gamma tubulin complexes was also observed in response to nanotopography. Furthermore, the addition of actin disrupting agents attenuated the alignment and proliferative effects of nanotopography. These findings further demonstrate the importance of interplay between cytoskeleton and substrate interactions as a key modulator of morphological and proliferative cellular response in hESCs on nanotopography.

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Microfabrication of poly (glycerol–sebacate) for contact guidance applications

Cited by 215 publications

References 30 publications

Enhancement of In Vitro Capillary Tube Formation by Substrate Nanotopography

Enhancement of In Vitro Capillary Tube Formation by Substrate Nanotopography

Recent advances in materials and flexible electronics for peripheral nerve interfaces

The effect of actin disrupting agents on contact guidance of human embryonic stem cells

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