Microfabrication with Very Low-Average Power of Green Light to Produce PDMS Microchips

Hernandez-Cedillo, Lucero M; Vázquez‐Cuevas, Francisco G.; Quintero-Torres, Rafael; Aragón, J. L.; Mortera, Miguel Angel Ocampo; Ordóñez‐Romero, César L.; Domínguez-Juárez, Jorge Luis

doi:10.3390/polym13040607

Cited by 3 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the fact that cell seeding will be performed manually and the analysis will be conducted without the use of a bioreactor or microfluidic device, the channel width and depth are set in macro dimensions. 28 Membranes with channel patterns were macroscopically photographed and microscopically examined using SEM analysis, and channel dimensions were measured. The resulting images were presented.…”

Section: Methodsmentioning

confidence: 99%

Surface channel patterned and endothelialized poly(glycerol sebacate) based elastomers

et al. 2022

View full text Add to dashboard Cite

Prevascularization of tissue equivalents is critical for fulfilling the need for sufficient vascular organization for nutrient and gas transport. Hence, endothelial cell culture on biomaterials is of great importance for researchers. Numerous alternate strategies have been suggested in this sense, with cell-based methods being the most commonly employed. In this study, poly (glycerol sebacate) (PGS) elastomers with varying crosslinking ratios were synthesized and their surfaces were patterned with channels by using laser ablation technique. In order to determine an ideal material for cell culture studies, the elastomers were subsequently mechanically, chemically, and biologically characterized. Following that, human umbilical vein endothelial cells (HUVECs) were seeded into the channels established on the PGS membranes and cultured under various culture conditions to establish the optimal culture parameters. Lastly, the endothelial cell responses to the synthesized PGS elastomers were evaluated. Remarkable cell proliferation and impressive cellular organizations were noticed on the constructs created as part of the investigation. On the concrete output of this research, arrangements in various geometries can be created by laser ablation method and the effects of various molecules, drugs or agents on endothelial cells can be evaluated. The platforms produced can be employed as an intermediate biomaterial layer containing endothelial cells for vascularization of tissue-engineered structures, particularly in layer-by-layer tissue engineering approaches.

show abstract

Section: Methodsmentioning

confidence: 99%

Surface channel patterned and endothelialized poly(glycerol sebacate) based elastomers

et al. 2022

View full text Add to dashboard Cite

show abstract

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

et al. 2023

View full text Add to dashboard Cite

Novel and improved biocatalysts are increasingly sourced from libraries via experimental screening. The success of such campaigns is crucially dependent on the number of candidates tested. Water-in-oil emulsion droplets can replace the classical test tube, to provide in vitro compartments as an alternative screening format, containing genotype and phenotype and enabling a readout of function. The scale-down to micrometer droplet diameters and picoliter volumes brings about a >10 7 -fold volume reduction compared to 96-well-plate screening. Droplets made in automated microfluidic devices can be integrated into modular workflows to set up multistep screening protocols involving various detection modes to sort >10 7 variants a day with kHz frequencies. The repertoire of assays available for droplet screening covers all seven enzyme commission (EC) number classes, setting the stage for widespread use of droplet microfluidics in everyday biochemical experiments. We review the practicalities of adapting droplet screening for enzyme discovery and for detailed kinetic characterization. These new ways of working will not just accelerate discovery experiments currently limited by screening capacity but profoundly change the paradigms we can probe. By interfacing the results of ultrahigh-throughput droplet screening with next-generation sequencing and deep learning, strategies for directed evolution can be implemented, examined, and evaluated. CONTENTSAA 11.6.2. Combining High-Throughput Selections with High-Throughput Analysis AB 12. Conclusions

show abstract

Actively Q-switched self-frequency-doubled Yb:YCOB green laser with output power of a dozen watts

Du,

Chen,

et al. 2023

Opt. Lett.

View full text Add to dashboard Cite

A diode laser (LD) end-pumped acousto-optic Q-switched self-frequency-doubled (SFD) Yb:YCa4O(BO3)3 (Yb:YCOB) pulsed green laser was realized for the first time (to our knowledge), with a maximum average output power of 11.6 W and an optical conversion efficiency of 30.0% from the LD to SFD lasers. The wavelength of the SFD lasers was controlled to be 507 nm for improving quantum efficiency up to 96% and reducing thermal effects. The repetition rates ranged from 20 to 500 kHz, and the maximum pulse energy was 312.0 µJ with a peak power of 4.78 kW at a repetition rate of 20 kHz. This work represents the highest output power in the SFD pulsed lasers and provides an efficient and compact way to generate high-power pulsed green lasers that should have important applications in many aspects, such as ultraviolet (UV) laser generation, laser display, and medical, military, and scientific research.

show abstract

Microfabrication with Very Low-Average Power of Green Light to Produce PDMS Microchips

Cited by 3 publications

References 41 publications

Surface channel patterned and endothelialized poly(glycerol sebacate) based elastomers

Surface channel patterned and endothelialized poly(glycerol sebacate) based elastomers

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

Actively Q-switched self-frequency-doubled Yb:YCOB green laser with output power of a dozen watts

Contact Info

Product

Resources

About