Microflora in the honeybee digestive tract: counts, characteristics and sensitivity to veterinary drugs

Rada, V.; Machová, M.; Huk, J.; Marounek, M.; Dušková, Dagmar

doi:10.1051/apido:19970603

Cited by 63 publications

(52 citation statements)

References 5 publications

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“…Comparable results were published by Kačániová et al (2004). In contrast to the study of Rada et al (1997), Kačániová et al (2004) did not detect lactobacilli and staphylococci. Piccini et al (2004) described an approach for the characterization of the cultivable heterotrophic bacterial flora of honey bee hives.…”

Section: Introductioncontrasting

confidence: 49%

“…Microorganisms of that type, mostly Bacillus spp. are commonly found on honey bees, honey bee larvae, in honey bee gastrointestinal tract as well as in honey and the honey bees environment (Smolska-Szymczewska 1989;Snowdon and Cliver 1996;Gilliam 1997;Rada et al 1997;Kačániová et al 2004;Piccini et al 2004;Iurlina and Fritz 2005;Mohr and Tebbe 2006;Evans and Armstrong 2006;Mohr and Tebbe 2007;Babendreier et al 2007;López and Alippi 2007;Kačániová et al 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Snowdon and Cliver (1996) and Gilliam (1997) reviewed about microorganisms in honey and the role of nonpathogenic microbiota, respectively. Rada et al (1997) reported about the microorganism in the honey bee digestive tract. In that study, the microorganisms were enumerated and characterized by means of classical phenotyping.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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-This study was conducted to improve the knowledge about bacteria associated with honey bees, Apis mellifera carnica. In this survey, the diversity of Gram-negative non-fermenting bacteria isolated and cultivated from pollen loads, honey sac, freshly stored nectar, and honey was investigated. Bacteria were characterized by a polyphasic approach. Based on morphological and physiological characteristics and comparison of isolates protein patterns after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 11 protein similarity groups were established and confirmed by enterobacterial repetitive intergenic consensus PCR. One isolate, representing a protein similarity group (representative strain), was characterized in more detail by analysis of respiratory quinones, amplified ribosomal DNA restriction analysis, and 16S rDNA sequence analysis. Based on the results of these examinations, seven representative strains were identified as members of the genus Pseudomonas. The remaining representative strains were allocated to the genera Acinetobacter, Chryseobacterium, Stenotrophomonas, and Commamonas.bacterial diversity / PCR / honey / honey bees / 16S rDNA gene

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Section: Introductioncontrasting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

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Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

et al. 2011

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“…The microbiota associated to the honeybee A. mellifera is complex, and it has been described as being mainly composed of yeasts, Gram-positive bacteria (such as Lactobacillus rigidus apis, S. constellatus, Bacillus spp., Streptococcus, and Clostridium), and Gram-negative or Gram-variable bacteria (Achromobacter, Citrobacter, Enterobacter, Erwinia, Escherichia coli, Flavobacterium, Klebsiella, Proteus, and Pseudomonas) [54][55][56][57][58].…”

Section: The Gut Microbiota As An Environmental Factor For Honeybee Hmentioning

confidence: 99%

Microorganisms in Honey

Silva¹,

Rabadzhiev²,

Eller³

et al. 2017

Honey Analysis

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Honey is a product with low water activity because of the great amount of sugars (fructose and glucose), and also it has antimicrobial compounds derived from flowers or because of its transformation process in the beehive. Despite all the honey microorganism barriers, some species of microorganisms are able to survive and may cause damage to honeybees or consumers. Techniques of pathogenic microorganism identification by DNA using PCR are recommended and required for sanitary and customs control. It is important to know the diversity of contaminating microorganisms in honey, especially due to disseminate pathogenic microorganisms in the international traded marketing. In contrast, beneficial microorganisms such as yeasts can remain latently in this product waiting for the moment in which the environment is suitable for their development. Among the beneficial bacteria found in honeybee products, we can mention some lactic acid bacteria that act as prebiotics when ingested. The microorganisms in the digestive tract of honeybees are important for their health. Thus, we present the knowledge of microbiota associated with honey from honeybees and stingless bees (Hymenoptera, Apidae) and the techniques available for the detection of microorganisms in honey.

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“…The anterior hindgut region is the most densely inhabited part of the digestive system, due to the availability of partially digested food coming from the midgut, as well as the products excreted by the Malpighian tubules. It has been estimated that 10 8 to 10 9 bacterial cells per g of gut content are present in the honeybee gut (39,57), while Ͼ10 6 bacteria are typically recovered from an entire old Drosophila fly (58).…”

Section: Which Insect Organs Do Aab Inhabit?mentioning

confidence: 99%

Acetic Acid Bacteria, Newly Emerging Symbionts of Insects

Crotti

Rizzi

Chouaia

et al. 2010

Appl Environ Microbiol

300

260

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8Recent research in microbe-insect symbiosis has shown that acetic acid bacteria (AAB) establish symbiotic relationships with several insects of the orders Diptera, Hymenoptera, Hemiptera, and Homoptera, all relying on sugar-based diets, such as nectars, fruit sugars, or phloem sap. To date, the fruit flies Drosophila melanogaster and Bactrocera oleae, mosquitoes of the genera Anopheles and Aedes, the honey bee Apis mellifera, the leafhopper Scaphoideus titanus, and the mealybug Saccharicoccus sacchari have been found to be associated with the bacterial genera Acetobacter, Gluconacetobacter, Gluconobacter, Asaia, and Saccharibacter and the novel genus Commensalibacter. AAB establish symbiotic associations with the insect midgut, a niche characterized by the availability of diet-derived carbohydrates and oxygen and by an acidic pH, selective factors that support AAB growth. AAB have been shown to actively colonize different insect tissues and organs, such as the epithelia of male and female reproductive organs, the Malpighian tubules, and the salivary glands. This complex topology of the symbiosis indicates that AAB possess the keys for passing through body barriers, allowing them to migrate to different organs of the host. Recently, AAB involvement in the regulation of innate immune system homeostasis of Drosophila has been shown, indicating a functional role in host survival. All of these lines of evidence indicate that AAB can play different roles in insect biology, not being restricted to the feeding habit of the host. The close association of AAB and their insect hosts has been confirmed by the demonstration of multiple modes of transmission between individuals and to their progeny that include vertical and horizontal transmission routes, comprising a venereal one. Taken together, the data indicate that AAB represent novel secondary symbionts of insects.

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Microflora in the honeybee digestive tract: counts, characteristics and sensitivity to veterinary drugs

Cited by 63 publications

References 5 publications

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica)

Microorganisms in Honey

Acetic Acid Bacteria, Newly Emerging Symbionts of Insects

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