Microflora of boxes for holding veterinary patients in clinics

Mocherniuk, M. M.; Kukhtyn, Mykola; Horiuk, Yulia; Horiuk, V. V.; Tsvigun, O. A.; Tokarchuk, T. S.

doi:10.15421/022233

Cited by 6 publications

(6 citation statements)

References 54 publications

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“…Earlier studies (Mocherniuk et al, 2022) found that the quantitative content of mesophilic bacteria in the bioaerosol of the premises of veterinary clinics increases during the working day. Furthermore, there is a significant difference in the number of bacteria in different rooms of the clinic, which depends on the intensity of operation of the rooms and the therapeutic and preventive manipulations that are carried out in them.…”

Section: Resultsmentioning

confidence: 97%

“…The control of nosocomial infection in medical and veterinary institutions is crucial in the system of preventing the spread of antibiotic-resistant strains among patients and in the environment. Closed premises of veterinary clinics often create conditions for the circulation and transmission of nosocomial infection via airborne bioaerosol (Jeong et al, 2022;Mocherniuk et al, 2022;Zheng et al, 2023). Nosocomial infection is considered a common cause of complications of the underlying disease among companion animals in veterinary clinics and quite often leads to mortality (Elnageh et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Identification of the bioaerosol microbiota in veterinary clinics as the key to preventing nosocomial infection

Mocherniuk¹,

Kukhtyn²,

Horiuk³

et al. 2022

Scientific Horizons

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Nosocomial infections are important in veterinary medicine clinics, as they can contaminate surfaces and infect animals through bioaerosol and are the cause of ineffective antibiotic therapy. This paper presents a study of the effect of disinfection on the microbiota of bioaerozoic veterinary clinics. The purpose of this study was to establish the composition of the bioaerosol microbiota in veterinary clinic premises before and after disinfection with ultraviolet bactericidal lamps. Bioaerosol samples were taken in the premises of veterinary clinics by sedimentation method. Identification of the isolated microbiota was performed using classical methods involving commercial test systems for identifying microorganisms. It was established that the permanent microbiota of the bioaerosol of veterinary clinics includes the following representatives of gram-positive genera: Staphylococcus (coagulase-negative species), Streptococcus spp., Micrococcus spp., and Corynebacterium spp. These bacterial genera were present in the bioaerosol of all rooms in 100% of cases. Gram-negative species of bacteria were found in small quantities in the bioaerosol of such rooms as for the primary examination and the manipulation area with boxes for keeping sick animals. Representatives of gram-negative species were detected in a considerably larger number from the bioaerosol of the dental operating room during the day of the clinic. After disinfection with bactericidal lamps, pathogens of nosocomial infections (S. aureus, S. pseudintermedius, Acinetobacter baumani, P. aeruginosa) were released in the bioaerosol of such rooms as the primary examination, the manipulation area with boxes for keeping sick animals, and the dental operating room. This indicates that bioaerosol can serve as a medium for the spread of nosocomial infections among animals in veterinary clinics. Thus, conducting microbiological monitoring of bioaerosol in veterinary clinics will allow identifying pathogens of nosocomial infections and introducing preventive measures for the spread of pathogens

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Identification of the bioaerosol microbiota in veterinary clinics as the key to preventing nosocomial infection

Mocherniuk¹,

Kukhtyn²,

Horiuk³

et al. 2022

Scientific Horizons

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“…Аерозолі свиноферм можуть містити велику кількість бактерій, грибків, вірусів, спор, метаболітів, генів резистентності тощо (Mocherniuk et al, 2022). Дослідженнями встановлено, що великі аерозольні частинки в повітрі свиноферми можуть затримуватися понад 5 с на висоті 1,5 м (Wang et al, 2021), а гени резистентності в повітрі можуть поширюватися з потоком вітру на відстань до 10 км, збільшуючи можливість інфікування людей і тварин (Bai et al, 2022).…”

Section: таблицяunclassified

“…Склад мікрофлори біоаерозолів може різнитися між собою залежно від географічного положення, клімату, пори року, типу свинарників, режиму годівлі та утримання тварин (Mocherniuk et al, 2022). Проте багато дослідників виявили, що переважаюча кількість мікроорганізмів представлена відділом бактерій Firmicutes (близько 62 %) та Bacteroidetes (<7 %) (Liu et al, 2019;Tang et al, 2022).…”

Section: таблицяunclassified

Characterization of harmful gases and bioaerosols of pig farms: a review of the existing literature

Grigorash,

Horiuk

2024

SMLNUVMB

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From the ecological and economic point of view, intensive pig farming is drawing more and more attention due to the appearance of harmful substances, which significantly increases the risks of air, water and soil pollution. In addition, the optimal criteria for the content of harmful gases, dust and microorganisms in the environment of pig farms are currently not clearly defined. In order to better understand the interaction of harmful emissions and aerosols, the article reviews data from the literature on their main components, concentrations, and interactions. The results showed that the main pollutants in pig farming are harmful gases (ammonia (NH3), carbon dioxide (CO2), methane (CH4), nitrogen oxide (N2O), hydrogen sulfide (H2S), which are released from manure. The presence of even small concentrations of harmful gases in farm air can cause respiratory and cardiovascular system disorders not only in animals, but also in humans. Along with harmful gases, it is important to control the emission of solid particles and dust from livestock premises, which can form aerosols. Microbial aerosols in pig houses contain bacteria, fungi, viruses, which mainly come from the animals themselves, manure or service personnel. The bioaerosol includes the Firmicutes and Bacteroidetes bacterial divisions and their derivatives. In addition, the content of potentially dangerous bacteria in the bioaerosol can reach up to 40 %. A major concern causes the presence of a large number of antibiotic-resistant microorganisms in the air of these farms (including MRSA). The existing strategies and methods to combat these problems are still imperfect and need to be refined. Currently, the interaction of harmful gases, dust, solid particles and microorganisms is not taken into account, which can increase the toxic effect of each other on the animal's body. Therefore, there is a need for a better understanding of these interactions in order to improve the strategy for improving the microclimate conditions by correcting the microbiota, finding and developing biological preparations that contain natural bacteria capable of neutralizing odors and disinfecting livestock premises.

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“…Наприклад, дезінфекція приміщень у клініках чи ветеринарних кабінетах передбачає використання бактерицидних ламп (Sinha et al, 2020). Такий спосіб економічно вигідний і також можливо обробити велику площу за порівняно короткий час (Mocherniuk et al, 2022). Знищення збудників на птахофабриках чи у ветеринарних тваринницьких господарствах зручніше проводити аерозольним способом та за присутності тварин у корівниках, клітках і т. д.…”

Section: результати та їх обговоренняunclassified

Resistance of a mixed bacterial culture isolated from bee dysbiosis to disinfectant in laboratory conditions

Lakhman,

Romanishina,

Zastulka

et al. 2024

SMLNUVMB

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Disinfection is one of the components of preventive actions to prevent disease in animals by infectious agents. Through the elimination of agents of infectious diseases in the environment, it is able to interrupt the mechanisms of the epizootic process. For the development of beekeeping and obtaining quality and products, it is necessary to carry out activities in apiaries, which include disinfection. The introduction of new disinfectants in the field is possible after preliminary laboratory tests. The aim of our research objective to investigate the resistance of a mixed bacterial culture isolated from bees with the symptoms of dysbiosis to disinfectant under laboratory conditions. Bacteriological investigations carried out in Petri dishes using meat-peptone agar and Mueller-Hinton agar. The disinfectant diluted with distilled water at concentrations of 0.25 %, 0.5 %, 1,25 % and 2,5 %. On the Mueller Hinton agar, the bacterial elimination zones recorded at 1.25 % (11,6 ± 0,45 mm) and 2.5 % (12,4 ± 0,27 mm) concentration of disinfectant at 1 day of investigation, and 7,4 ± 0,27 mm (1,25 %), 11,2 ± 0,42 mm (2.5 %) at 72 hours of the experiment, respectively. In addition, the bacterial growth inhibition effect increased with experimental time and ranged from 24.0 ± 0.35 mm (0.5 %) to 28.2 ± 0.22 mm (2.5 %) on day 3 of the study. Bacteriostatic and bactericidal effects appeared to be less pronounced on meat-peptone agar. The highest diameters of inhibition of the processes of reproduction and development of microorganisms from mixed microbial association on this medium were recorded at 1.25 % and 2.5 % concentrations of the disinfectant at the 3rd day of the investigation with diameters of the zones of inhibition of growth 15.6 ± 0.45 mm and 26.4 ± 0.27 mm, respectively. The specificity of diffusion of the investigated active substance from the discs into the agar medium thickness was also noted. This effect has a varying effect on infectious agents in in vitro experiments. Thus bacteriological laboratory experiments should be performed on several culture media in purpose of obtaining accurate results. The analysis of the obtained results indicates the demonstration of antimicrobial effect of the investigated disinfectant against mixed bacterial culture in the form of bactericidal and bacteriostatic actions. Therefore, a promising direction of research for veterinary doctors and apiary business owners is the search for effective and efficient disinfectants. Laboratory investigation of the activity of a remedy are the first step before it is approbation.

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Microflora of boxes for holding veterinary patients in clinics

Cited by 6 publications

References 54 publications

Identification of the bioaerosol microbiota in veterinary clinics as the key to preventing nosocomial infection

Identification of the bioaerosol microbiota in veterinary clinics as the key to preventing nosocomial infection

Characterization of harmful gases and bioaerosols of pig farms: a review of the existing literature

Resistance of a mixed bacterial culture isolated from bee dysbiosis to disinfectant in laboratory conditions

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