Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy

Liu, Dongfei; Zhang, Hongbo; Mäkilä, Ermei; Fan, Jin; Herranz-Blanco, Bárbara; Wang, Chang-Fang; Rosa, Ricardo Figueiredo; Ribeiro, Antônio J.; Salonen, Jarno; Hirvonen, Jouni; Santos, Hélder A.

doi:10.1016/j.biomaterials.2014.10.079

Cited by 133 publications

(120 citation statements)

References 53 publications

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“…During the experiment the following were used: acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7, acetylcholinesterase from human erythrocytes), acetylthiocholine iodide, 5,5 -dithio-bis(2-nitrobenzoic acid), neostigmine methyl sulfate and MgCl 2 , purchased from Sigma-Aldrich, NaCl (Daejung Chemical and Metals Co., Ltd), ethanol, water (Samchun Chemicals), HF (48 %, w/w; Merck) and boron-doped p-type silicon wafers with a resistivity of [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] cm and thickness 500-550 μm (obtained from Cree Co.). The photoluminescence spectra and relative photoluminescence intensities were measured on an FS-2 fluorescence spectrometer (Scinco) and LabRam HR-800 spectrometer (Horiba Jobin Yvon) with a He/Cd laser source (325 nm).…”

Section: Materials and Instrumentationmentioning

confidence: 99%

“…The silicon wafer (boron-doped p-type silicon wafers with resistivity [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] cm and thickness 500-550 μm) was cut into chips sized 1×1 cm 2 and degreased with an ultrasonic bath of acetone for 5 min, and then rinsed in deionized water. After drying with nitrogen gas, silver paste was simply sputtered on to the back of the wafer to provide a back ohmic contact for anodization.…”

Section: Preparation Of Porous Silicon Surfacementioning

confidence: 99%

“…The unique features of porous silicon make this material a frontline candidate for enzyme immobilization [3][4][5][6][7][8][9], drug delivery [10,11], energyharvesting devices [12][13][14][15][16][17] and biosensing [18,19], due to its large internal surface area, prodigious pore volume, biodegradability, and tunable pore geometry via variation of both porosity and crystallite size [20][21][22][23][24]. The biodegradation of porous silicon results in the formation of orthosilicic acid, which can easily be absorbed from the gastrointestinal tract and excreted from the body [25].…”

Section: Introductionmentioning

confidence: 99%

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Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

et al. 2016

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SynopsisA successful prescription is presented for acetylcholinesterase physically adsorbed on to a mesoporous silicon surface, with a promising hydrolytic response towards acetylthiocholine iodide. The catalytic behaviour of the immobilized enzyme was assessed by spectrophotometric bioassay using neostigmine methyl sulfate as a standard acetycholinesterase inhibitor. The surface modification was studied through field emission SEM, Fourier transform IR spectroscopy, energy-dispersive X-ray spectroscopy, cathode luminescence and X-ray photoelectron spectroscopy analysis, photoluminescence measurement and spectrophotometric bioassay. The porous silicon-immobilized enzyme not only yielded greater enzyme stability, but also significantly improved the native photoluminescence at room temperature of the bare porous silicon architecture. The results indicated the promising catalytic behaviour of immobilized enzyme compared with that of its free counterpart, with a greater stability, and that it aided reusability and easy separation from the reaction mixture. The porous silicon-immobilized enzyme was found to retain 50 % of its activity, promising thermal stability up to 90 • C, reusability for up to three cycles, pH stability over a broad pH of 4-9 and a shelf-life of 44 days, with an optimal hydrolytic response towards acetylthiocholine iodide at variable drug concentrations. On the basis of these findings, it was believed that the porous silicon-immobilized enzyme could be exploited as a reusable biocatalyst and for screening of acetylcholinesterase inhibitors from crude plant extracts and synthesized organic compounds. Moreover, the immobilized enzyme could offer a great deal as a viable biocatalyst in bioprocessing for the chemical and pharmaceutical industries, and bioremediation to enhance productivity and robustness.

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Section: Materials and Instrumentationmentioning

confidence: 99%

Section: Preparation Of Porous Silicon Surfacementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

et al. 2016

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“…Acid-responsive drug delivery would be beneficial for cancer therapy due to the acidic tumor microenvironment. [27][28][29][30] To show the acid-triggered release of Dox from the NPs, TSH-SiO 2 @Dox-NPs were put into dialysis bags (molecular weight cut-off 3,500 Da) and then incubated in acetate-buffered solution (pH =5.0) and phosphate-buffered solution (PBS; pH =7.4), respectively. These two pH values at 5.0 and 7.4 could simulate the pH values in the blood circulation 31 and within tumor cells.…”

Section: Resultsmentioning

confidence: 99%

Targeting thyroid cancer with acid-triggered release of doxorubicin from silicon dioxide nanoparticles

Li¹,

Zhang²,

Sheng

et al. 2017

IJN

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Currently, therapy for thyroid cancer mainly involves surgery and radioiodine therapy. However, chemotherapy can be used in advanced and aggressive thyroid cancer that cannot be treated by other options. Nevertheless, a major obstacle to the successful treatment of thyroid cancer is the delivery of drugs to the thyroid gland. Here, we present an example of the construction of silicon dioxide nanoparticles with thyroid–stimulating-hormone receptor-targeting ligand that can specifically target the thyroid cancer. Doxorubicin nanoparticles can be triggered by acid to release the drug payload for cancer therapy. These nanoparticles shrink the tumor size in vivo with less toxic side effects. This research paves the way toward effective chemotherapy for thyroid cancer.

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“…For controlled synthesis and formulation of microcapsules [1] or polymeric nanoparticles [2][3][4] in a microfluidic device, various reactions are modulated in the capillary flow. For particlesurface or surface-surface interactions mediated by the action of receptors and ligands, the adhesion can compete with rotational mobility of particles, intervening tether dynamics [5][6][7], and size or shape-dependent margination effects [8,9].…”

Section: Introductionmentioning

confidence: 99%

Effect of wall-mediated hydrodynamic fluctuations on the kinetics of a Brownian nanoparticle

Eckmann

Ayyaswamy

et al. 2016

Proc. R. Soc. A.

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.436049)) and the subsequent development of methods such as transition path sampling have laid the foundation for explicitly quantifying the rate process in terms of microscopic simulations. However, explicit methods to account for how the hydrodynamic correlations impact the transient reaction rate are missing in the colloidal literature. We show that the composite generalized Langevin equation (Yu et al. 2015 Phys. Rev. E 91, 052303. (doi:10.1103/PhysRevE.91.052303)) makes a significant step towards solving the coupled processes of molecular reactions and hydrodynamic relaxation by examining how the wall-mediated hydrodynamic memory impacts the two-stage temporal relaxation of the reaction rate for a nanoparticle transition between two bound states in the bulk, near-wall and lubrication regimes.

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Microfluidic assisted one-step fabrication of porous silicon@acetalated dextran nanocomposites for precisely controlled combination chemotherapy

Cited by 133 publications

References 53 publications

Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

Targeting thyroid cancer with acid-triggered release of doxorubicin from silicon dioxide nanoparticles

Effect of wall-mediated hydrodynamic fluctuations on the kinetics of a Brownian nanoparticle

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