Microfluidic-based screening of resveratrol and drug-loading PLA/Gelatine nano-scaffold for the repair of cartilage defect

Abstract

Cartilage defect is common in clinical but notoriously difficult to treat for low regenerative and migratory capacity of chondrocytes. Biodegradable tissue engineering nano-scaffold with a lot of advantages has been the direction of material to repair cartilage defect in recent years. The objective of our study is to establish a biodegradable drug-loading synthetic polymer (PLA) and biopolymer (Gelatine) composite 3D nano-scaffold to support the treatment of cartilage defect. We designed a microfluidic chip-based drug-screening device to select the optimum concentration of resveratrol, which has strong protective capability for chondrocyte. Then biodegradable resveratrol-loading PLA/Gelatine 3D nano-scaffolds were fabricated and used to repair the cartilage defects. As a result, we successfully cultured primary chondrocytes and screened the appropriate concentrations of resveratrol by the microfluidic device. We also smoothly obtained superior biodegradable resveratrol-loading PLA/Gelatine 3D nano-scaffolds and compared the properties and therapeutic effects of cartilage defect in rats. In summary, our microfluidic device is a simple but efficient platform for drug screening and resveratrol-loading PLA/Gelatine 3D nano-scaffolds could greatly promote the cartilage formation. It would be possible for materials and medical researchers to explore individualized pharmacotherapy and drug-loading synthetic polymer and biopolymer composite tissue engineering scaffolds for the repair of cartilage defect in future.

Green Synthesis of CeO2 Nanoparticles from the Abelmoschus esculentus Extract: Evaluation of Antioxidant, Anticancer, Antibacterial, and Wound-Healing Activities

“…Nanobiotechnology is an advanced and emerging field of science and technology consist of metals and metal oxides at the nano range. In addition, nanobiotechnology has many applications, especially in the biomedical field [ 1 , 2 , 3 ]. CeO 2 nanoparticles (NPs) have attained significant consideration in nanomedicine because of their promising applications in drug delivery, catalysis, biosensing, and medicine.…”

Synthesis and characterization of levan hydrogels and their use for resveratrol release

“…They concluded that, with certain amount of resveratrol in scaffolds, cell proliferation increased due to resveratrols’ strong protective and damage reducing properties. 59 Zhao et al prepared peptide hydrogels with slow resveratrol release and NIH 3T3 fibroblasts and RAW 264.7 macrophage cells were used for in vitro testing and no cytotoxic effect was observed with anti-inflammatory effect. In vivo scar models on adult male Sprague–Dawley rats showed reduced scar formation and collagen deposition thus hydrogels are concluded to have a potential usage as wound dressings.…”

Evaluation of Trans-Resveratrol as a Treatment for Periodontitis

“…For example, in a 2021 study, Li and colleagues produced nano-hydroxyapatite-RES-chitosan (CS) microspheres for bone generation, which could potentially be used to restore bone loss due to PD [133]. *Address all correspondence to: tharney@college.bm Also, electrospun 3-D nano-scaffolds loaded with RES, consisting of a biodegradable polymer (PLA)-biopolymer-gelatin (GEL) nano-scaffold was found to repair cartilage defects in the rat model [134].…”