Microfluidic Biomaterials

Tien, Joe; Dance, Yoseph W.

doi:10.1002/adhm.202001028

Cited by 28 publications

(16 citation statements)

References 123 publications

(241 reference statements)

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“…In addition, it can be more difficult to work with these systems given the fragility of the gel and the sometimes long culture periods of weeks. However, these systems have enormous potential, and the interested reader is directed to the excellent review in Reference 70 .…”

Section: Past Present and New Challenges For Engineered In Vitro Microvasculaturementioning

confidence: 99%

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Myers

Lam

2021

Annu. Rev. Biomed. Eng.

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Microengineering advances have enabled the development of perfusable, endothelialized models of the microvasculature that recapitulate the unique biological and biophysical conditions of the microcirculation in vivo. Indeed, at that size scale (<100 μm)—where blood no longer behaves as a simple continuum fluid; blood cells approximate the size of the vessels themselves; and complex interactions among blood cells, plasma molecules, and the endothelium constantly ensue—vascularized microfluidics are ideal tools to investigate these microvascular phenomena. Moreover, perfusable, endothelialized microfluidics offer unique opportunities for investigating microvascular diseases by enabling systematic dissection of both the blood and vascular components of the pathophysiology at hand. We review ( a) the state of the art in microvascular devices and ( b) the myriad of microvascular diseases and pressing challenges. The engineering community has unique opportunities to innovate with new microvascular devices and to partner with biomedical researchers to usher in a new era of understanding and discovery of microvascular diseases. Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 23 is June 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Section: Past Present and New Challenges For Engineered In Vitro Microvasculaturementioning

confidence: 99%

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Myers

Lam

2021

Annu. Rev. Biomed. Eng.

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“…[204] Biomedical micro-hydrogels seeded with cells are conventionally fabricated via relatively direct, rapid, and simple manual procedures. [205,206] One typical example is the micromolding which is typically employed because of its operational simplicity. Nevertheless, in such a method, large quantities of samples cannot be generated, and the hydrogel is at risk of being damaged and distorted during certain phases of the process (like demolding).…”

Section: Fabrication Of Structured Biomaterialsmentioning

confidence: 99%

“…[232,[252][253][254] In parallel, novel biomaterials and strategies have been developed to structurally control the scaffolds employed as a support for cell growth. [205] In fact, one can direct the internal porosity and interconnectivity of the scaffolds to create matrices that are highly permissive to the fluid flows. In the next subsections, we will briefly discuss how microfluidics has been or could be applied to solve problems connected to tissue perfusion and vascularization.…”

Section: Media Distribution Within Tissuementioning

confidence: 99%

Microfluidic Tissue Engineering and Bio‐Actuation

et al. 2022

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Bio‐hybrid technologies aim to replicate the unique capabilities of biological systems that could surpass advanced artificial technologies. Soft bio‐hybrid robots consist of synthetic and living materials and have the potential to self‐assemble, regenerate, work autonomously, and interact safely with other species and the environment. Cells require a sufficient exchange of nutrients and gases, which is guaranteed by convection and diffusive transport through liquid media. The functional development and long‐term survival of biological tissues in vitro can be improved by dynamic flow culture, but only microfluidic flow control can develop tissue with fine structuring and regulation at the microscale. Full control of tissue growth at the microscale will eventually lead to functional macroscale constructs, which are needed as the biological component of soft bio‐hybrid technologies. This review summarizes recent progress in microfluidic techniques to engineer biological tissues, focusing on the use of muscle cells for robotic bio‐actuation. Moreover, the instances in which bio‐actuation technologies greatly benefit from fusion with microfluidics are highlighted, which include: the microfabrication of matrices, biomimicry of cell microenvironments, tissue maturation, perfusion, and vascularization.

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“…Decellularized ECM is mainly composed of collagen and can be digested by pepsin to make a hydrogel, which can be injected in vivo for tissue regeneration or processed in vitro for tissue engineering [8–10]. More recently, microfluidics has been used to build microchannels, which are seeded with endothelial cells, to mimic microvascular structure [6, 8, 11–15]. The endothelial cells can also be mixed in a 3D hydrogel and self‐assemble to form a microvascular network within the microfluidic channels [16, 17].…”

Section: Introductionmentioning

confidence: 99%

Patterned vascularization in a directional ice‐templated scaffold of decellularized matrix

Song

et al. 2021

Engineering in Life Sciences

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Vascularization is fundamental for large‐scale tissue engineering. Most of the current vascularization strategies including microfluidics and three‐dimensional (3D) printing aim to precisely fabricate microchannels for individual microvessels. However, few studies have examined the remodeling capacity of the microvessels in the engineered constructs, which is important for transplantation in vivo. Here we present a method for patterning microvessels in a directional ice‐templated scaffold of decellularized porcine kidney extracellular matrix. The aligned microchannels made by directional ice templating allowed for fast and efficient cell seeding. The pure decellularized matrix without any fixatives or cross‐linkers maximized the potential of tissue remodeling. Dramatical microvascular remodeling happened in the scaffold in 2 weeks, from small primary microvessel segments to long patterned microvessels. The majority of the microvessels were aligned in parallel and interconnected with each other to form a network. This method is compatible with other engineering techniques, such as microfluidics and 3D printing, and multiple cell types can be co‐cultured to make complex vascularized tissue and organ models.

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Microfluidic Biomaterials

Cited by 28 publications

References 123 publications

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Microfluidic Tissue Engineering and Bio‐Actuation

Patterned vascularization in a directional ice‐templated scaffold of decellularized matrix

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