Microfluidic chemical processing with on-chip washing by deterministic lateral displacement arrays with separator walls

Chen, Yu; D'Silva, Joseph; Austin, Robert H.; Sturm, James C.

doi:10.1063/1.4930863

Cited by 7 publications

(7 citation statements)

References 20 publications

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“…[41] with permission from Springer Nature (CC BY 2017), Ref. [85] with permission from AIP Publishing, copyright 2015, Ref. [20] with permission from Springer Nature, copyright 2016 and various biological samples like blood cells, bacteria, exosomes.…”

Section: Applications Of Size-based Separationmentioning

confidence: 99%

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A Review on Deterministic Lateral Displacement for Particle Separation and Detection

2019

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HIGHLIGHTS• A well-organized and thorough discussion on the fundamental principles and recent progress in deterministic lateral displacement (DLD) is provided.• The most updated designs and applications of DLD techniques for particle separation and detection are reviewed.• The current limitations of DLD and its potential solutions for clinical and commercial applications are discussed.ABSTRACT The separation and detection of particles in suspension are essential for a wide spectrum of applications including medical diagnostics. In this field, microfluidic deterministic lateral displacement (DLD) holds a promise due to the ability of continuous separation of particles by size, shape, deformability, and electrical properties with high resolution. DLD is a passive microfluidic separation technique that has been widely implemented for various bioparticle separations from blood cells to exosomes. DLD techniques have been previously reviewed in 2014. Since then, the field has matured as several physics of DLD have been updated, new phenomena have been discovered, and various designs have been presented to achieve a higher separation performance and throughput. Furthermore, some recent progress has shown new clinical applications and ability to use the DLD arrays as a platform for biomolecules detection. This review provides a thorough discussion on the recent progress in DLD with the topics based on the fundamental studies on DLD models and applications for particle separation and detection. Furthermore, current challenges and potential solutions of DLD are also discussed. We believe that a comprehensive understanding on DLD techniques could significantly contribute toward the advancements in the field for various applications. In particular, the rapid, low-cost, and high-throughput particle separation and detection with DLD have a tremendous impact for point-of-care diagnostics.

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Section: Applications Of Size-based Separationmentioning

confidence: 99%

“…b The two-stage DLD arrangement based on both the size and asymmetry for CTC clusters separation [ 41 ]. c The on-chip microfluidic DLD for chemical treatment and washing [ 85 ]. d The condenser array in a nano-DLD shows the zigzag, intermediate, and bumping modes for different nanoparticle diameters and gap sizes [ 20 ].…”

Section: Dld Applicationsmentioning

confidence: 99%

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

2019

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“…This so-called real time particle washing is one objective of the particle medium exchange step [152]. A number of microfluidic approaches have been developed to achieve on-chip particle medium exchange [153,154,155,156]. Below we first present a short summary on the biocompatibility of magnetic fluids.…”

Section: Particle Medium Exchangementioning

confidence: 99%

Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Xuan¹

2019

Micromachines

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Magnetic field-induced particle manipulation is simple and economic as compared to other techniques (e.g., electric, acoustic, and optical) for lab-on-a-chip applications. However, traditional magnetic controls require the particles to be manipulated being magnetizable, which renders it necessary to magnetically label particles that are almost exclusively diamagnetic in nature. In the past decade, magnetic fluids including paramagnetic solutions and ferrofluids have been increasingly used in microfluidic devices to implement label-free manipulations of various types of particles (both synthetic and biological). We review herein the recent advances in this field with focus upon the continuous-flow particle manipulations. Specifically, we review the reported studies on the negative magnetophoresis-induced deflection, focusing, enrichment, separation, and medium exchange of diamagnetic particles in the continuous flow of magnetic fluids through microchannels.

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“…Hence, it is considered that on-chip sample preparation or processing on separated flows can be realized by controlling each flow stream, using DLD structures inside the microfluidic channels. For example, the staining of leukocytes via Rhodamine dye and a subsequent washing process was demonstrated by using DLD arrays [35].…”

Section: Working Principlesmentioning

confidence: 99%

Sequential Cell-Processing System by Integrating Hydrodynamic Purification and Dielectrophoretic Trapping for Analyses of Suspended Cancer Cells

et al. 2019

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Microfluidic devices employing dielectrophoresis (DEP) have been widely studied and applied in the manipulation and analysis of single cells. However, several pre-processing steps, such as the preparation of purified target samples and buffer exchanges, are necessary to utilize DEP forces for suspended cell samples. In this paper, a sequential cell-processing device, which is composed of pre-processing modules that employ deterministic lateral displacement (DLD) and a single-cell trapping device employing an electroactive microwell array (EMA), is proposed to perform the medium exchange followed by arraying single cells sequentially using DEP. Two original microfluidic devices were efficiently integrated by using the interconnecting substrate containing rubber gaskets that tightly connect the inlet and outlet of each device. Prostate cancer cells (PC3) suspended in phosphate-buffered saline buffer mixed with microbeads were separated and then resuspended into the DEP buffer in the integrated system. Thereafter, purified PC3 cells were trapped in a microwell array by using the positive DEP force. The achieved separation and trapping efficiencies exceeded 94% and 93%, respectively, when using the integrated processing system. This study demonstrates an integrated microfluidic device by processing suspended cell samples, without the requirement of complex preparation steps.

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Microfluidic chemical processing with on-chip washing by deterministic lateral displacement arrays with separator walls

Cited by 7 publications

References 20 publications

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

A Review on Deterministic Lateral Displacement for Particle Separation and Detection

Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Sequential Cell-Processing System by Integrating Hydrodynamic Purification and Dielectrophoretic Trapping for Analyses of Suspended Cancer Cells

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