Microfluidic chips as a method for sperm selection improve fertilization rate in couples with fertilization failure

Mirsanei, Jamileh Sadat; Sheibak, Nadia; Zandieh, Zahra; Mehdizadeh, Mehdi; Aflatoonian, Reza; Tabatabaei, Maryamsadat; Mousavi, Atieh Sadat; Amjadi, Fatemehsadat

doi:10.1007/s00404-022-06618-w

Cited by 19 publications

(10 citation statements)

References 42 publications

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“…Sperm that accumulate on specific parts of the device are selected. [195][196][197][198][199][200] Hyaluronic acid-based selection Functional sperm present specific receptors that allow them to bind to hyaluronic acid.…”

Section: Discussionmentioning

confidence: 99%

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Soto-Heras

Sakkas

Miller

2023

Biology of Reproduction

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The contribution of sperm to embryogenesis is gaining attention with up to 50% of infertility cases being attributed to a paternal factor. The traditional methods used in assisted reproductive technologies (ART) for selecting and assessing sperm quality are mainly based on motility and viability parameters. However, other sperm characteristics, including DNA integrity, have major consequences for successful live birth. In natural reproduction, sperm navigate the male and female reproductive tract to reach and fertilize the egg. During transport, sperm encounter many obstacles that dramatically reduce the number arriving at the fertilization site. In humans, the number of sperm is reduced from tens of millions in the ejaculate to hundreds in the Fallopian tube (oviduct). Whether this sperm population has higher fertilization potential is not fully understood, but several studies in animals indicate that many defective sperm do not advance to the site of fertilization. Moreover, the oviduct plays a key role in fertility by modulating sperm transport, viability, and maturation, providing sperm that are ready to fertilize at the appropriate time. Here we present evidence of sperm selection by the oviduct with emphasis on the mechanisms of selection and the sperm characteristics selected. Considering the sperm parameters that are essential for healthy embryonic development, we discuss the use of novel in vitro sperm selection methods that mimic physiological conditions. We propose that insight gained from understanding how the oviduct selects sperm can be translated to ART to yield high fertilization, embryonic development, and pregnancy rates.

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Section: Discussionmentioning

confidence: 99%

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Soto-Heras

Sakkas

Miller

2023

Biology of Reproduction

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“…Calisici et al (2019) (conference paper) and Li et al (2016) reported that the rate of reaching the blastocyst increased after the use of microfluidic sperm sorting device in IVEP in cattle, and there was an increase of 11%–16% compared to the control group. In many in vitro fertilization studies in humans, it has been reported that microfluidic sperm sorting devices increase the rate of reaching the blastocyst and the number of quality embryos (Anbari et al, 2021; Guler et al, 2021; Mirsanei et al, 2022). Choosing morphologically better quality and motile spermatozoa allows for increased sperm capacitation ability and, as a result, more successful fertilization with oocyte (Mirsanei et al, 2022; Yildiz & Yuksel, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In many in vitro fertilization studies in humans, it has been reported that microfluidic sperm sorting devices increase the rate of reaching the blastocyst and the number of quality embryos (Anbari et al, 2021; Guler et al, 2021; Mirsanei et al, 2022). Choosing morphologically better quality and motile spermatozoa allows for increased sperm capacitation ability and, as a result, more successful fertilization with oocyte (Mirsanei et al, 2022; Yildiz & Yuksel, 2019). Accordingly, the use of microfluidic sperm sorting devices allows the selection of spermatozoa that are more suitable for in vitro fertilization (Anbari et al, 2021; Asghar et al, 2014; Ogata et al, 2021; Pujol et al, 2022; Quinn et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, it was determined that there was a significant increase in the cell number and quality of the blastocysts developed after using a microfluidic sperm sorting chip in the fertilization step of IVEP in cattle. Anbari et al (2021), Guler et al (2021), and Mirsanei et al (2022) reported that embryo quality increased after sperm selection with a microfluidic sperm sorting device before ICSI application. Many factors affect embryo quality in cattle (Demetrio et al, 2020; Lonergan et al, 2001; Sartori et al, 2010), and possible effects of spermatozoa processing and selection methodologies are among them (Anbari et al, 2021; Henkel & Schill, 2003).…”

Section: Discussionmentioning

confidence: 99%

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Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?

Alkan

Satılmış

Demirel

et al. 2023

Reprod Domestic Animals

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This study aimed to determine the effect of microfluidic sperm sorting chip on embryo development and quality in the sperm treatment step in in vitro embryo production in cattle. Only A-quality oocytes obtained from the ovaries of Holstein cattle were included in the study. These oocytes were first placed in in vitro maturation medium, and matured oocytes were randomly divided into two groups at the 24th hour of maturation. Oocytes in the first group with the Microfluidic Sperm Sorting Chip (MFSC, n = 154) were put into a fertilization medium with spermatozoa prepared with microfluidic sperm sorting device. Oocytes in the second group (Con, n = 169) were fertilized with spermatozoa prepared by the routine sperm treatment step of the commercial company. The rate of cleavage (85.71% vs. 76.33%, respectively) and of reaching the blastocyst (44.15% vs. 32.54%, respectively) in the MFSC group were higher than the control group. In addition, it was determined that the numbers of ICM (45.8 ± 2.04 vs. 39.2 ± 1.85, respectively), TE (122.13 ± 2.19 vs. 115.0 ± 2.61, respectively), TC (167.93 ± 2.89 vs. 154.2 ± 2.62, respectively) increased in the MFSC group compared to the control group. A statistically significant difference was found in the number of cells with apoptosis per embryo (5.14 ± 0.77 vs. 11.91 ± 0.79) and apoptotic index rates (3.06 ± 0.47 vs. 7.72 ± 0.55%) of the MFSC and Con groups. As a result, we concluded that using microfluidic sperm sorting chips during sperm treatment in bovine IVEP increases the rate of reaching the blastocyst, embryo development, and quality and reduces the possibility of apoptosis in developing blastocysts. For this reason, it is also thought that the use of microfluidic sperm sorting devices during sperm treatment in bovine IVEP may be a new alternative in this field.

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“…In 2022, Mirsanei et al sought to re-evaluate the question of whether or not MSS could be beneficial for couples with a history of low fertilization rate (<25%) or total fertilization failure during a prior IVF cycle compared to those undergoing their first ICSI cycle [ 99 ]. Couples were excluded if the male partner had a history of an abnormal semen analysis, varicocele, or other known cause of male infertility.…”

Section: Methods For Overcoming High Sdfmentioning

confidence: 99%

Sperm DNA Damage and Its Relevance in Fertility Treatment: A Review of Recent Literature and Current Practice Guidelines

Marinaro

2023

IJMS

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Sperm deoxyribonucleic acid (DNA) damage has recently emerged as one of the most controversial topics in male reproductive medicine. While level I evidence indicates that abnormal sperm DNA damage has substantial adverse effects on reproductive outcomes (including chance of pregnancy and risk of miscarriage), there is limited consensus on how sperm DNA fragmentation (SDF) testing should be performed and/or interpreted in clinical practice. In this article, we review: (1) how SDF is assessed, (2) cumulative evidence regarding its impact on reproductive outcomes, (3) methods for mitigating high SDF, and (4) the most recent practice guidelines available for clinicians regarding the use and interpretation of SDF testing.

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Microfluidic chips as a method for sperm selection improve fertilization rate in couples with fertilization failure

Cited by 19 publications

References 42 publications

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?

Sperm DNA Damage and Its Relevance in Fertility Treatment: A Review of Recent Literature and Current Practice Guidelines

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